LMNB2 Antibody - #DF12209

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Product: LMNB2 Antibody
Catalog: DF12209
Description: Rabbit polyclonal antibody to LMNB2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 66-68kd; 70kD(Calculated).
Uniprot: Q03252
RRID: AB_2845014

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Zebrafish(83%), Bovine(83%), Horse(80%), Sheep(83%), Dog(83%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
LMNB2 Antibody detects endogenous levels of total LMNB2.
RRID:
AB_2845014
Cite Format: Affinity Biosciences Cat# DF12209, RRID:AB_2845014.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

LAMB 2; LAMB2; Lamin-B2; LMN 2; LMN B2; LMN2; LMNB 2; LMNB2; LMNB2_HUMAN; MGC2721; RGD1563803;

Immunogens

Immunogen:
Uniprot:

Q03252(LMNB2_HUMAN) >>Visit HPA database.

Gene(ID):
LMNB2(84823)
Sequence:
MSPPSPGRRREQRRPRAAATMATPLPGRAGGPATPLSPTRLSRLQEKEELRELNDRLAHYIDRVRALELENDRLLLKISEKEEVTTREVSGIKALYESELADARRVLDETARERARLQIEIGKLRAELDEVNKSAKKREGELTVAQGRVKDLESLFHRSEVELAAALSDKRGLESDVAELRAQLAKAEDGHAVAKKQLEKETLMRVDLENRCQSLQEELDFRKSVFEEEVRETRRRHERRLVEVDSSRQQEYDFKMAQALEELRSQHDEQVRLYKLELEQTYQAKLDSAKLSSDQNDKAASAAREELKEARMRLESLSYQLSGLQKQASAAEDRIRELEEAMAGERDKFRKMLDAKEQEMTEMRDVMQQQLAEYQELLDVKLALDMEINAYRKLLEGEEERLKLSPSPSSRVTVSRATSSSSGSLSATGRLGRSKRKRLEVEEPLGSGPSVLGTGTGGSGGFHLAQQASASGSVSIEEIDLEGKFVQLKNNSDKDQSLGNWRIKRQVLEGEEIAYKFTPKYILRAGQMVTVWAAGAGVAHSPPSTLVWKGQSSWGTGESFRTVLVNADGEEVAMRTVKKSSVMRENENGEEEEEEAEFGEEDLFHQQGDPRTTSRGCYVM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
83
Bovine
83
Sheep
83
Dog
83
Zebrafish
83
Chicken
83
Horse
80
Xenopus
67
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q03252 As Substrate

Site PTM Type Enzyme
T23 Phosphorylation
R28 Methylation
T34 Phosphorylation
S37 Phosphorylation P24941 (CDK2)
T39 Phosphorylation P24941 (CDK2)
S42 Phosphorylation
K47 Acetylation
K47 Ubiquitination
R56 Methylation
Y60 Phosphorylation
R73 Methylation
K77 Acetylation
K77 Ubiquitination
S79 Phosphorylation
K81 Acetylation
K81 Ubiquitination
K93 Ubiquitination
Y96 Phosphorylation
S98 Phosphorylation
K123 Ubiquitination
K133 Ubiquitination
S134 Phosphorylation
K150 Acetylation
K150 Ubiquitination
S154 Phosphorylation
S168 Phosphorylation
K170 Acetylation
K170 Ubiquitination
R171 Methylation
S175 Phosphorylation
K186 Acetylation
K186 Ubiquitination
K195 Acetylation
K200 Acetylation
K200 Ubiquitination
R211 Methylation
S214 Phosphorylation
K223 Ubiquitination
S224 Phosphorylation
S246 Phosphorylation
K255 Ubiquitination
R264 Methylation
S265 Phosphorylation
R272 Methylation
K275 Ubiquitination
Y282 Phosphorylation
K285 Ubiquitination
K290 Acetylation
K290 Ubiquitination
K298 Acetylation
K298 Ubiquitination
S301 Phosphorylation
K308 Ubiquitination
S316 Phosphorylation
S318 Phosphorylation
Y319 Phosphorylation
S322 Phosphorylation
K326 Ubiquitination
R334 Methylation
K351 Ubiquitination
Y374 Phosphorylation
K393 Acetylation
K393 Sumoylation
K393 Ubiquitination
R401 Methylation
K403 Sumoylation
K403 Ubiquitination
S405 Phosphorylation
S407 Phosphorylation
S409 Phosphorylation
S410 Phosphorylation
T413 Phosphorylation
S415 Phosphorylation
T418 Phosphorylation
S419 Phosphorylation
S420 Phosphorylation
S421 Phosphorylation
S422 Phosphorylation
S424 Phosphorylation
S426 Phosphorylation
T428 Phosphorylation
K489 Ubiquitination
S492 Phosphorylation
K494 Ubiquitination
S497 Phosphorylation
Y515 Phosphorylation
K516 Ubiquitination
T518 Phosphorylation
K520 Acetylation
K520 Ubiquitination
S541 Phosphorylation
S552 Phosphorylation
T556 Phosphorylation
S559 Phosphorylation

Research Backgrounds

Function:

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

PTMs:

B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

Subcellular Location:

Nucleus inner membrane>Lipid-anchor>Nucleoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with TMEM43.

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

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