Product: FAF2 Antibody
Catalog: DF12202
Description: Rabbit polyclonal antibody to FAF2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 53 kDa; 53kD(Calculated).
Uniprot: Q96CS3
RRID: AB_2845007

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
FAF2 Antibody detects endogenous levels of total FAF2.
RRID:
AB_2845007
Cite Format: Affinity Biosciences Cat# DF12202, RRID:AB_2845007.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI462440; expressed in T cells and eosinophils in atopic dermatitis; faf2; FAF2_HUMAN; Fas associated factor family member 2; FAS-associated factor 2; KIAA0887; Protein ETEA; Protein expressed in T cells and eosinophils in atopic dermatitis; Protein expressed in T-cells and eosinophils in atopic dermatitis; UBX domain containing 8; UBX domain protein 3B; UBX domain-containing protein 3B; UBX domain-containing protein 8; UBXD8; UBXN3B;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q96CS3 FAF2_HUMAN:

Broadly expressed, with highest levels in brain.

Sequence:
MAAPEERDLTQEQTEKLLQFQDLTGIESMDQCRHTLEQHNWNIEAAVQDRLNEQEGVPSVFNPPPSRPLQVNTADHRIYSYVVSRPQPRGLLGWGYYLIMLPFRFTYYTILDIFRFALRFIRPDPRSRVTDPVGDIVSFMHSFEEKYGRAHPVFYQGTYSQALNDAKRELRFLLVYLHGDDHQDSDEFCRNTLCAPEVISLINTRMLFWACSTNKPEGYRVSQALRENTYPFLAMIMLKDRRMTVVGRLEGLIQPDDLINQLTFIMDANQTYLVSERLEREERNQTQVLRQQQDEAYLASLRADQEKERKKREERERKRRKEEEVQQQKLAEERRRQNLQEEKERKLECLPPEPSPDDPESVKIIFKLPNDSRVERRFHFSQSLTVIHDFLFSLKESPEKFQIEANFPRRVLPCIPSEEWPNPPTLQEAGLSHTEVLFVQDLTDE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
67
Zebrafish
60
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96CS3 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T10 Phosphorylation
T14 Phosphorylation
K16 Ubiquitination
S66 Phosphorylation
Y79 Phosphorylation
S80 Phosphorylation
Y81 Phosphorylation
S142 Phosphorylation
K146 Ubiquitination
T158 Phosphorylation
K167 Acetylation
K167 Ubiquitination
K215 Ubiquitination
T244 Phosphorylation
Y297 Phosphorylation
S300 Phosphorylation
K307 Ubiquitination
K321 Ubiquitination
K329 Ubiquitination
K343 Ubiquitination
K346 Ubiquitination
S355 Phosphorylation
K363 Ubiquitination
K367 Ubiquitination
K395 Ubiquitination
K400 Ubiquitination

Research Backgrounds

Function:

Plays an important role in endoplasmic reticulum-associated degradation (ERAD) that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway. Involved in inhibition of lipid droplet degradation by binding to phospholipase PNPL2 and inhibiting its activity by promoting dissociation of PNPL2 from its endogenous activator, ABHD5 which inhibits the rate of triacylglycerol hydrolysis.

Subcellular Location:

Cytoplasm. Lipid droplet. Endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Broadly expressed, with highest levels in brain.

Subunit Structure:

Identified in a complex that contains SEL1L, OS9, FAF2/UBXD8, UBE2J1/UBC6E and AUP1. Interacts with YOD1. Interacts (via N-terminus) with UBQLN2 (via C-terminus). Interacts with PNPLA2 and UBAC2. Interacts with ZFAND2B; probably through VCP. Interacts with LMBR1L.

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