CYP26B1 Antibody - #DF12194

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Product: CYP26B1 Antibody
Catalog: DF12194
Description: Rabbit polyclonal antibody to CYP26B1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 58-70 kDa; 58kD(Calculated).
Uniprot: Q9NR63
RRID: AB_2844999

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
CYP26B1 Antibody detects endogenous levels of total CYP26B1.
RRID:
AB_2844999
Cite Format: Affinity Biosciences Cat# DF12194, RRID:AB_2844999.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CP26; CP26B_HUMAN; CYP26A2; cyp26b1; Cytochrome P450 26A2; Cytochrome P450 26B1; Cytochrome P450 family 26 subfamily B polypeptide 1; Cytochrome P450 retinoic acid-inactivating 2; Cytochrome P450 retinoid metabolizing protein; Cytochrome P450 subfamily XXVIA, polypeptide 2; Cytochrome P450 subfamily XXVIB polypeptide 1; Cytochrome P450RAI-2; DKFZp686G0638; dol; EC 1.14.; fc21d03; MGC129613; P450 26A2; P450 retinoic acid inactivating 2; P450RAI 2; P450RAI2; Retinoic acid metabolizing cytochrome; Retinoic acid-metabolizing cytochrome; RHFCA; stocksteif; wu:fc21d03; wu:fc26h10; zgc:76999;

Immunogens

Immunogen:
Uniprot:

Q9NR63(CP26B_HUMAN) >>Visit HPA database.

Gene(ID):
CYP26B1(56603)
Expression:
Q9NR63 CP26B_HUMAN:

Highly expressed in brain, particularly in the cerebellum and pons.

Sequence:
MLFEGLDLVSALATLAACLVSVTLLLAVSQQLWQLRWAATRDKSCKLPIPKGSMGFPLIGETGHWLLQGSGFQSSRREKYGNVFKTHLLGRPLIRVTGAENVRKILMGEHHLVSTEWPRSTRMLLGPNTVSNSIGDIHRNKRKVFSKIFSHEALESYLPKIQLVIQDTLRAWSSHPEAINVYQEAQKLTFRMAIRVLLGFSIPEEDLGHLFEVYQQFVDNVFSLPVDLPFSGYRRGIQARQILQKGLEKAIREKLQCTQGKDYLDALDLLIESSKEHGKEMTMQELKDGTLELIFAAYATTASASTSLIMQLLKHPTVLEKLRDELRAHGILHSGGCPCEGTLRLDTLSGLRYLDCVIKEVMRLFTPISGGYRTVLQTFELDGFQIPKGWSVMYSIRDTHDTAPVFKDVNVFDPDRFSQARSEDKDGRFHYLPFGGGVRTCLGKHLAKLFLKVLAVELASTSRFELATRTFPRITLVPVLHPVDGLSVKFFGLDSNQNEILPETEAMLSATV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Pig
92
Bovine
92
Sheep
92
Dog
92
Xenopus
75
Zebrafish
67
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NR63 As Substrate

Site PTM Type Enzyme
K275 Ubiquitination
T290 Phosphorylation
Y298 Phosphorylation
T317 Phosphorylation
K321 Ubiquitination
S462 Phosphorylation
T470 Phosphorylation

Research Backgrounds

Function:

Involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation. Involved in the specific inactivation of all-trans-retinoic acid (all-trans-RA), with a preference for the following substrates: all-trans-RA > 9-cis-RA > 13-cis-RA. Generates several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA, and 18-OH-RA. Catalyzes the hydroxylation of carbon hydrogen bonds of atRA primarily at C-4. Essential for postnatal survival. Plays a central role in germ cell development: acts by degrading RA in the developing testis, preventing STRA8 expression, thereby leading to delay of meiosis. Required for the maintenance of the undifferentiated state of male germ cells during embryonic development in Sertoli cells, inducing arrest in G0 phase of the cell cycle and preventing meiotic entry. Plays a role in skeletal development, both at the level of patterning and in the ossification of bone and the establishment of some synovial joints.

Has also a significant activity in oxidation of tazarotenic acid and may therefore metabolize that xenobiotic in vivo.

Subcellular Location:

Endoplasmic reticulum membrane>Peripheral membrane protein. Microsome membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in brain, particularly in the cerebellum and pons.

Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Curc-mPEG454, a PEGylated curcumin derivative, as a multi-target anti-fibrotic prodrug. International immunopharmacology, 2021 (PubMed: 34628270) [IF=5.6]

Application: WB    Species: Rat    Sample: liver tissues

Fig. 5. Curc-mPEG454 up-regulates AOX1 and down-regulates CYP26A1 and CYP26B1 to restore retinoic acid (RA) level. (A) Heatmap represents the gene expression of Retinol metabolism. Genes related to retinol synthesis and RA metabolism are indicated. (B) Paired display the gene expression by RNA-seq and relative mRNA expression by qRT-PCR from RA metabolism. Gene expression level was measured in FPKM value. The mRNA levels were normalized to GAPDH, and subsequently represents as fold change relative to control group. (C) The protein levels of CYP26A1 and CYP26B1 and AOX1. (D) RA contents detected by ELISA in liver tissues. Data are expressed as mean ± SEM. #P < 0.05, ##P < 0.01 compared with normal group. *P < 0.05, **P < 0.01, ***P < 0.001 compared with CCl4-induced model group.

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