Product: CPLX1 Antibody
Catalog: DF12192
Description: Rabbit polyclonal antibody to CPLX1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 15-20 kDa; 15kD(Calculated).
Uniprot: O14810
RRID: AB_2844997

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(85%)
Clonality:
Polyclonal
Specificity:
CPLX1 Antibody detects endogenous levels of total CPLX1.
RRID:
AB_2844997
Cite Format: Affinity Biosciences Cat# DF12192, RRID:AB_2844997.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

921-S; complexin 1; Complexin I; Complexin-1; CPLX1; CPLX1_HUMAN; CPX I; CPX-I; CPX1; Synaphin 2; Synaphin-2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O14810 CPLX1_HUMAN:

Nervous system. In hippocampus and cerebellum, expressed mainly by inhibitory neurons. Overexpressed in substantia nigra from patients with Parkinson disease.

Sequence:
MEFVMKQALGGATKDMGKMLGGDEEKDPDAAKKEEERQEALRQAEEERKAKYAKMEAEREAVRQGIRDKYGIKKKEEREAEAQAAMEANSEGSLTRPKKAIPPGCGDEVEEEDESILDTVIKYLPGPLQDMLKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
85
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14810 As Substrate

Site PTM Type Enzyme
K14 Acetylation
T95 Phosphorylation
S115 Phosphorylation

Research Backgrounds

Function:

Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles. Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse. Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. Essential for motor behavior.

Subcellular Location:

Cytoplasm>Cytosol. Perikaryon. Cell junction>Synapse>Presynapse.
Note: Enriched at synaptic-releasing sites in mature neurons.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Nervous system. In hippocampus and cerebellum, expressed mainly by inhibitory neurons. Overexpressed in substantia nigra from patients with Parkinson disease.

Subunit Structure:

Binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A.

Family&Domains:

Belongs to the complexin/synaphin family.

Research Fields

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

References

1). Punicalagin protects against impaired skeletal muscle function in high-fat-diet-induced obese mice by regulating TET2. Food & Function, 2023 (PubMed: 36929898) [IF=6.1]

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