CKAP4 Antibody - #DF12190

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Product: CKAP4 Antibody
Catalog: DF12190
Description: Rabbit polyclonal antibody to CKAP4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 50~70 kDa; 66kD(Calculated).
Uniprot: Q07065
RRID: AB_2844995

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CKAP4 Antibody detects endogenous levels of total CKAP4.
RRID:
AB_2844995
Cite Format: Affinity Biosciences Cat# DF12190, RRID:AB_2844995.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

63 kDa membrane protein; CKAP 4; Ckap4; CKAP4_HUMAN; Cytoskeleton associated protein 4; Cytoskeleton-associated protein 4; ERGIC 63; ERGIC63; MGC99554; p63; Transmembrane protein 63kD endoplasmic reticulum Golgi intermediate compartment; Type II transmembrane protein p63;

Immunogens

Immunogen:
Uniprot:

Q07065(CKAP4_HUMAN) >>Visit HPA database.

Gene(ID):
CKAP4(10970)
Sequence:
MPSAKQRGSKGGHGAASPSEKGAHPSGGADDVAKKPPPAPQQPPPPPAPHPQQHPQQHPQNQAHGKGGHRGGGGGGGKSSSSSSASAAAAAAAASSSASCSRRLGRALNFLFYLALVAAAAFSGWCVHHVLEEVQQVRRSHQDFSRQREELGQGLQGVEQKVQSLQATFGTFESILRSSQHKQDLTEKAVKQGESEVSRISEVLQKLQNEILKDLSDGIHVVKDARERDFTSLENTVEERLTELTKSINDNIAIFTEVQKRSQKEINDMKAKVASLEESEGNKQDLKALKEAVKEIQTSAKSREWDMEALRSTLQTMESDIYTEVRELVSLKQEQQAFKEAADTERLALQALTEKLLRSEESVSRLPEEIRRLEEELRQLKSDSHGPKEDGGFRHSEAFEALQQKSQGLDSRLQHVEDGVLSMQVASARQTESLESLLSKSQEHEQRLAALQGRLEGLGSSEADQDGLASTVRSLGETQLVLYGDVEELKRSVGELPSTVESLQKVQEQVHTLLSQDQAQAARLPPQDFLDRLSSLDNLKASVSQVEADLKMLRTAVDSLVAYSVKIETNENNLESAKGLLDDLRNDLDRLFVKVEKIHEKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Pig
92
Bovine
92
Sheep
92
Rabbit
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q07065 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation
S9 Phosphorylation
S17 Phosphorylation
S19 Phosphorylation
K21 Ubiquitination
S26 Phosphorylation
K78 Ubiquitination
C100 S-Nitrosylation
S101 Phosphorylation
S140 Phosphorylation
S145 Phosphorylation
K188 Ubiquitination
S195 Phosphorylation
S198 Phosphorylation
K206 Ubiquitination
K213 Ubiquitination
K223 Ubiquitination
T231 Phosphorylation
S232 Phosphorylation
T236 Phosphorylation
K270 Acetylation
K283 Ubiquitination
K287 Ubiquitination
K301 Ubiquitination
S312 Phosphorylation
T313 Phosphorylation
T316 Phosphorylation
Y322 Phosphorylation
T323 Phosphorylation
S330 Phosphorylation
K355 Ubiquitination
K405 Ubiquitination
S427 Phosphorylation
S436 Phosphorylation
S460 Phosphorylation
S461 Phosphorylation
K490 Ubiquitination
S492 Phosphorylation
T512 O-Glycosylation
S515 Phosphorylation
S534 Phosphorylation
S535 Phosphorylation
K540 Ubiquitination
K551 Methylation
K551 Ubiquitination
Y563 Phosphorylation
S576 Phosphorylation
K578 Ubiquitination
R590 Methylation

Research Backgrounds

Function:

High-affinity epithelial cell surface receptor for APF.

Mediates the anchoring of the endoplasmic reticulum to microtubules.

PTMs:

Reversibly palmitoylated. Palmitoylation at Cys-100 by DHHC2 is required for its trafficking from the ER to the plasma membrane and for its perinuclear localization.

Increased phosphorylation during mitosis prevents binding to microtubules.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type II membrane protein. Cell membrane>Single-pass type II membrane protein. Cytoplasm>Cytoskeleton. Cytoplasm>Perinuclear region.
Note: Translocates to the perinuclear region upon APF-stimulation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

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