USP39 Antibody - #DF12180

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Product: USP39 Antibody
Catalog: DF12180
Description: Rabbit polyclonal antibody to USP39
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Sheep, Dog
Mol.Wt.: 65 kDa; 65kD(Calculated).
Uniprot: Q53GS9
RRID: AB_2844985

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Sheep(83%), Dog(100%)
Clonality:
Polyclonal
Specificity:
USP39 Antibody detects endogenous levels of total USP39.
RRID:
AB_2844985
Cite Format: Affinity Biosciences Cat# DF12180, RRID:AB_2844985.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

65K; CGI 21; CGI-21; FLJ33136; HSPC332; Inactive ubiquitin specific peptidase 39; Inactive ubiquitin-specific peptidase 39; MGC75069; PRO2855; S. CEREVISIAE, HOMOLOG OF SAD1; SAD1; SAD1 homolog; Small nuclear ribonucleoprotein 65kDa (U4/U6.U5); snRNP ASSEMBLY DEFECTIVE 1; SnRNP assembly defective 1 homolog; SNRNP65; SNUT2_HUMAN; U4/U6.U5 tri snRNP associated 65 kDa protein; U4/U6.U5 tri snRNP associated protein 2; U4/U6.U5 tri-snRNP-associated 65 kDa protein; U4/U6.U5 tri-snRNP-associated protein 2; Ubiquitin specific peptidase 39; Ubiquitin specific protease 39; USP39;

Immunogens

Immunogen:
Uniprot:

Q53GS9(SNUT2_HUMAN) >>Visit HPA database.

Gene(ID):
USP39(10713)
Sequence:
MSGRSKRESRGSTRGKRESESRGSSGRVKRERDREREPEAASSRGSPVRVKREFEPASAREAPASVVPFVRVKREREVDEDSEPEREVRAKNGRVDSEDRRSRHCPYLDTINRSVLDFDFEKLCSISLSHINAYACLVCGKYFQGRGLKSHAYIHSVQFSHHVFLNLHTLKFYCLPDNYEIIDSSLEDITYVLKPTFTKQQIANLDKQAKLSRAYDGTTYLPGIVGLNNIKANDYANAVLQALSNVPPLRNYFLEEDNYKNIKRPPGDIMFLLVQRFGELMRKLWNPRNFKAHVSPHEMLQAVVLCSKKTFQITKQGDGVDFLSWFLNALHSALGGTKKKKKTIVTDVFQGSMRIFTKKLPHPDLPAEEKEQLLHNDEYQETMVESTFMYLTLDLPTAPLYKDEKEQLIIPQVPLFNILAKFNGITEKEYKTYKENFLKRFQLTKLPPYLIFCIKRFTKNNFFVEKNPTIVNFPITNVDLREYLSEEVQAVHKNTTYDLIANIVHDGKPSEGSYRIHVLHHGTGKWYELQDLQVTDILPQMITLSEAYIQIWKRRDNDETNQQGA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Sheep
83
Bovine
67
Pig
63
Rabbit
63
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q53GS9 As Substrate

Site PTM Type Enzyme
S42 Phosphorylation
S46 Phosphorylation
S58 Phosphorylation
S65 Phosphorylation
S82 Phosphorylation
S97 Phosphorylation
C105 S-Nitrosylation
K207 Ubiquitination
K210 Ubiquitination
Y259 Phosphorylation
K260 Ubiquitination
K283 Ubiquitination
K291 Methylation
K308 Ubiquitination
K428 Acetylation
K428 Ubiquitination
K434 Ubiquitination
K445 Ubiquitination
K466 Ubiquitination
R481 Methylation
K508 Ubiquitination

Research Backgrounds

Function:

Plays a role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the precatalytic spliceosome. Regulates AURKB mRNA levels, and thereby plays a role in cytokinesis and in the spindle checkpoint. Does not have ubiquitin-specific peptidase activity.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

The U4/U6-U5 tri-snRNP complex is a building block of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8.

Family&Domains:

Belongs to the peptidase C19 family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

References

1). USP39 Promotes the Viability and Migration of Head and Neck Squamous Cell Carcinoma Cell by Regulating STAT1. Technology in cancer research & treatment, 2024 (PubMed: 38706215) [IF=2.8]

Application: IHC    Species: Human    Sample: neck squamous cell carcinoma (HNSCC) tumor and adjacent tissues

Figure 3. USP39 and STAT1 expression levels in head and neck squamous cell carcinoma (HNSCC) tumor and adjacent tissues. (A) Representative immunohistochemical images and statistical summary data for USP39 levels in HNSCC tumor and adjacent tissues (AT). The effect size between the two groups is 1.39348 (95% CI: 0.44, 2.32). (B) Representative immunohistochemical images and statistical summary data for STAT1 levels in HNSCC tumor and adjacent tissue. The effect size between the two groups is 1.21 (95% CI: 0.28, 2.11). Data are shown as the mean ± SEM; n = 11. *P 

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