Syntaxin 11 Antibody - #DF12174

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Product: Syntaxin 11 Antibody
Catalog: DF12174
Description: Rabbit polyclonal antibody to Syntaxin 11
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 35 kDa; 33kD(Calculated).
Uniprot: O75558
RRID: AB_2844979

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(92%), Chicken(83%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
Syntaxin 11 Antibody detects endogenous levels of total Syntaxin 11.
RRID:
AB_2844979
Cite Format: Affinity Biosciences Cat# DF12174, RRID:AB_2844979.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FHL4; HLH4; HPLH4; Stx11; STX11_HUMAN; Syntaxin 11; Syntaxin-11;

Immunogens

Immunogen:
Uniprot:

O75558(STX11_HUMAN) >>Visit HPA database.

Gene(ID):
STX11(8676)
Sequence:
MKDRLAELLDLSKQYDQQFPDGDDEFDSPHEDIVFETDHILESLYRDIRDIQDENQLLVADVKRLGKQNARFLTSMRRLSSIKRDTNSIAKAIKARGEVIHCKLRAMKELSEAAEAQHGPHSAVARISRAQYNALTLTFQRAMHDYNQAEMKQRDNCKIRIQRQLEIMGKEVSGDQIEDMFEQGKWDVFSENLLADVKGARAALNEIESRHRELLRLESRIRDVHELFLQMAVLVEKQADTLNVIELNVQKTVDYTGQAKAQVRKAVQYEEKNPCRTLCCFCCPCLK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
92
Xenopus
92
Chicken
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75558 As Substrate

Site PTM Type Enzyme
S75 Phosphorylation
S80 Phosphorylation
S81 Phosphorylation
S88 Phosphorylation
K103 Ubiquitination
S173 Phosphorylation
S190 Phosphorylation
K260 Ubiquitination

Research Backgrounds

Function:

SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network.

Subcellular Location:

Membrane>Peripheral membrane protein. Golgi apparatus>trans-Golgi network membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with the SNARE proteins SNAP-23 and VAMP.

Family&Domains:

Belongs to the syntaxin family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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