Synaptopodin Antibody - #DF12173

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Product: Synaptopodin Antibody
Catalog: DF12173
Description: Rabbit polyclonal antibody to Synaptopodin
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse
Mol.Wt.: 99-115 kDa; 118kD(Calculated).
Uniprot: Q9UMS6
RRID: AB_2844978

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%)
Clonality:
Polyclonal
Specificity:
Synaptopodin Antibody detects endogenous levels of total Synaptopodin.
RRID:
AB_2844978
Cite Format: Affinity Biosciences Cat# DF12173, RRID:AB_2844978.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686G051; Genethonin 2; Genethonin-2; Myopodin; striated muscle specific; Synaptopodin-2; SYNP2_HUMAN; SYNPO 2; Synpo2;

Immunogens

Immunogen:
Uniprot:

Q9UMS6(SYNP2_HUMAN) >>Visit HPA database.

Gene(ID):
SYNPO2(171024)
Expression:
Q9UMS6 SYNP2_HUMAN:

Expressed in heart muscle. Isoform 5 is specifically expressed in skeletal muscle.

Sequence:
MGTGDFICISMTGGAPWGFRLQGGKEQKQPLQVAKIRNQSKASGSGLCEGDEVVSINGNPCADLTYPEVIKLMESITDSLQMLIKRPSSGISEALISENENKNLEHLTHGGYVESTTLQIRPATKTQCTEFFLAPVKTEVPLAENQRSGPDCAGSLKEETGPSYQRAPQMPDSQRGRVAEELILREKVEAVQPGPVVELQLSLSQERHKGASGPLVALPGAEKSKSPDPDPNLSHDRIVHINSIPTNEKADPFLRSSKIIQISSGRELRVIQESEAGDAGLPRVEVILDCSDRQKTEGCRLQAGKECVDSPVEGGQSEAPPSLVSFAVSSEGTEQGEDPRSEKDHSRPHKHRARHARLRRSESLSEKQVKEAKSKCKSIALLLTDAPNPNSKGVLMFKKRRRRARKYTLVSYGTGELEREADEEEEGDKEDTCEVAFLGASESEVDEELLSDVDDNTQVVNFDWDSGLVDIEKKLNRGDKMEMLPDTTGKGALMFAKRRERMDQITAQKEEDKVGGTPSREQDAAQTDGLRTTTSYQRKEEESVRTQSSVSKSYIEVSHGLGHVPQQNGFSGTSETANIQRMVPMNRTAKPFPGSVNQPATPFSPTRNMTSPIADFPAPPPYSAVTPPPDAFSRGVSSPIAGPAQPPPWPQPAPWSQPAFYDSSERIASRDERISVPAKRTGILQEAKRRSTTKPMFTFKEPKVSPNPELLSLLQNSEGKRGTGAGGDSGPEEDYLSLGAEACNFMQSSSAKQKTPPPVAPKPAVKSSSSQPVTPVSPVWSPGVAPTQPPAFPTSNPSKGTVVSSIKIAQPSYPPARPASTLNVAGPFKGPQAAVASQNYTPKPTVSTPTVNAVQPGAVGPSNELPGMSGRGAQLFAKRQSRMEKYVVDSDTVQAHAARAQSPTPSLPASWKYSSNVRAPPPVAYNPIHSPSYPLAALKSQPSAAQPSKMGKKKGKKPLNALDVMKHQPYQLNASLFTFQPPDAKDGLPQKSSVKVNSALAMKQALPPRPVNAASPTNVQASSVYSVPAYTSPPSFFAEASSPVSASPVPVGIPTSPKQESASSSYFVAPRPKFSAKKSGVTIQVWKPSVVEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
67
Chicken
56
Pig
50
Sheep
50
Rabbit
50
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UMS6 As Substrate

Site PTM Type Enzyme
Y112 Phosphorylation
S155 Phosphorylation
S202 Phosphorylation
S212 Phosphorylation
S224 Phosphorylation
S226 Phosphorylation
S234 Phosphorylation
S243 Phosphorylation
S256 Phosphorylation
S257 Phosphorylation
S263 Phosphorylation
S264 Phosphorylation
S274 Phosphorylation
S310 Phosphorylation
S361 Phosphorylation
S363 Phosphorylation
S365 Phosphorylation
S374 Phosphorylation
S378 Phosphorylation
K398 Ubiquitination
T408 Phosphorylation
T506 Phosphorylation
T517 Phosphorylation
S519 Phosphorylation
S548 Phosphorylation
S551 Phosphorylation
T601 Phosphorylation
S604 Phosphorylation
T606 Phosphorylation
T610 Phosphorylation
S611 Phosphorylation
Y622 Phosphorylation
S623 Phosphorylation
T626 Phosphorylation
S633 Phosphorylation
S638 Phosphorylation
S675 Phosphorylation
K688 Ubiquitination
S691 Phosphorylation
T692 Phosphorylation
S705 Phosphorylation
S712 Phosphorylation
S717 Phosphorylation
K720 Acetylation
S729 Phosphorylation
S737 Phosphorylation
T755 Phosphorylation
K766 Acetylation
S767 Phosphorylation
S768 Phosphorylation
S769 Phosphorylation
S770 Phosphorylation
T774 Phosphorylation
S777 Phosphorylation
S781 Phosphorylation
T787 Phosphorylation
S798 Phosphorylation
T801 Phosphorylation
Y813 Phosphorylation
S820 Phosphorylation
Y840 Phosphorylation
Y886 Phosphorylation
T892 Phosphorylation
S902 Phosphorylation
T904 Phosphorylation
S906 Phosphorylation
S910 Phosphorylation
S930 Phosphorylation
S932 Phosphorylation
Y933 Phosphorylation
S998 Phosphorylation
S1041 Phosphorylation
S1042 Phosphorylation
S1047 Phosphorylation
T1055 Phosphorylation
S1056 Phosphorylation
Y1066 Phosphorylation
S1079 Phosphorylation

Research Backgrounds

Function:

Has an actin-binding and actin-bundling activity. Can induce the formation of F-actin networks in an isoform-specific manner. At the sarcomeric Z lines is proposed to act as adapter protein that links nascent myofibers to the sarcolemma via ZYX and may play a role in early assembly and stabilization of the Z lines. Involved in autophagosome formation. May play a role in chaperone-assisted selective autophagy (CASA) involved in Z lines maintenance in striated muscle under mechanical tension; may link the client-processing CASA chaperone machinery to a membrane-tethering and fusion complex providing autophagosome membranes (By similarity). Involved in regulation of cell migration. May be a tumor suppressor.

Involved in regulation of cell migration. Can induce formation of thick, irregular actin bundles in the cell body.

Involved in regulation of cell migration. Can induce long, well-organized actin bundles frequently orientated in parallel along the long axis of the cell showing characteristics of contractile ventral stress fibers.

Involved in regulation of cell migration. Can induce an amorphous actin meshwork throughout the cell body containing a mixture of long and short, randomly organized thick and thin actin bundles.

Can induce long, well-organized actin bundles frequently orientated in parallel along the long axis of the cell showing characteristics of contractile ventral stress fibers.

Involved in regulation of cell migration in part dependent on the Rho-ROCK cascade; can promote formation of nascent focal adhesions, actin bundles at the leading cell edge and lamellipodia. Can induce formation of thick, irregular actin bundles in the cell body; the induced actin network is associated with enhanced cell migration in vitro.

PTMs:

Phosphorylated by PKA, and by CaMK2 at multiple sites. Dephosphorylated by calcineurin; abrogating interaction with YWHAB and impairing nuclear import (By similarity). Phosphorylated by ILK.

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton. Cytoplasm>Myofibril>Sarcomere>Z line. Cell junction>Focal adhesion.
Note: Shuttles between the nucleus and the cytoplasm in a differentiation-dependent and stress-induced fashion. In undifferentiated myoblasts strongly expressed in the nucleus, after induction of myotube differentiation is located to both nucleus and cytoplasm along acting filaments, and in differentiated myotubes is located at the Z lines. Upon stress redistributes from cytoplasm of myoblasts and myotubes to the nucleus. Nuclear import is KPNA2-dependent and promoted by phosphorylation by PKA and/or CaMK2, and inhibition of calcineurin. The nuclear export is XPO1-dependent (By similarity). Localized in a fiber-like pattern, partly overlapping with filamentous actin (PubMed:18371299).

Cytoplasm>Cytoskeleton.
Note: Localizes to induced actin bundles with contiguous staining.

Cytoplasm>Cytoskeleton.
Note: Localizes to induced actin bundles with punctuate staining.

Cytoplasm>Cytoskeleton.
Note: Localizes to induced irregular actin bundles with contiguous and punctuated staining.

Cytoplasm>Cytoskeleton.
Note: Localizes to induced actin bundles with punctuate staining.

Cytoplasm>Cytoskeleton.
Note: Localizes to induced actin bundles with contiguous staining.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart muscle. Isoform 5 is specifically expressed in skeletal muscle.

Subunit Structure:

May self-associate in muscle cells under oxidative stress. Binds F-actin. Interacts with ACTN2; ACTN2 is proposed to anchor SYOP2 at Z lines in mature myocytes. Interacts with AKAP6, PPP3CA and CAMK2A. Interacts (phosphorylated form) with YWHAB; YWHAB competes with ACTN2 for interaction with SYNPO2. Interacts with KPNA2; mediating nuclear import of SYNOP2; dependent on interaction with YWHAB (By similarity). Interacts with IPO13; may be implicated in SYNOP2 nuclear import. Interacts with ZYX, FLNC, ILK. Interacts with BAG3 (via WW 1 domain). May associate with the CASA complex consisting of HSPA8, HSPB8 and BAG3. Interacts with VPS18.

Family&Domains:

The PPPY motif interacts with the WW domain 1 of BAG3.

Belongs to the synaptopodin family.

References

1). Silencing of PFKFB3 protects podocytes against high glucose‑induced injury by inducing autophagy. Molecular Medicine Reports, 2021 (PubMed: 34490476) [IF=3.4]

Application: WB    Species: Mouse    Sample: MPC5 cells

Figure 2. HG-induced cell viability inhibition and cell migration is reversed by PFKFB3 siRNA. MPC5 cells were treated with NC siRNA + NG, AR siRNA + NG, PFKFB3 siRNA + NG, NC siRNA + HG, AR siRNA + HG, PFKFB3 siRNA + HG or HG + mannitol for 48 h. (A) Cell viability was measured by Cell Counting Kit-8 assay. (B) Protein expression levels of synaptopodin in MPC5 cells were detected by western blot analysis and normalized to GAPDH. (C) Cell migration was examined by Transwell assay. *P<0.05, **P<0.01 vs. NC siRNA + NG; ^^P<0.01 vs. NC siRNA + HG. AR, aldose reductase; HG, high-glucose; NC, negative control; NG, normal glucose; PFKFB3, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3; siRNA, small interfering RNA.

Application: WB    Species: mouse    Sample: MPC5 cells

Figure 2.| HG‑induced cell viability inhibition and cell migration is reversed by PFKFB3 siRNA. MPC5 cells were treated with NC siRNA + NG, AR siRNA + NG, PFKFB3 siRNA + NG, NC siRNA + HG, AR siRNA + HG, PFKFB3 siRNA + HG or HG + mannitol for 48 h.(B) Protein expression levels of synaptopodin in MPC5 cells were detected by western blot analysis and normalized to GAPDH.

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