NASP Antibody - #DF12166
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12166, RRID:AB_2844971.
Fold/Unfold
5033430J04Rik; AI131596; AI317140; D4Ertd767e; DKFZp547F162; Epcs32; FLB7527; FLJ31599; FLJ35510; MGC19722; MGC20372; MGC2297; MGC93869; NASP; NASP_HUMAN; nuclear autoantigenic sperm protein (histone binding); Nuclear autoantigenic sperm protein; PRO1999; RP23 233B9.9;
Immunogens
- P49321 NASP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAMESTATAAVAAELVSADKIEDVPAPSTSADKVESLDVDSEAKKLLGLGQKHLVMGDIPAAVNAFQEAASLLGKKYGETANECGEAFFFYGKSLLELARMENGVLGNALEGVHVEEEEGEKTEDESLVENNDNIDEEAREELREQVYDAMGEKEEAKKTEDKSLAKPETDKEQDSEMEKGGREDMDISKSAEEPQEKVDLTLDWLTETSEEAKGGAAPEGPNEAEVTSGKPEQEVPDAEEEKSVSGTDVQEECREKGGQEKQGEVIVSIEEKPKEVSEEQPVVTLEKQGTAVEVEAESLDPTVKPVDVGGDEPEEKVVTSENEAGKAVLEQLVGQEVPPAEESPEVTTEAAEASAVEAGSEVSEKPGQEAPVLPKDGAVNGPSVVGDQTPIEPQTSIERLTETKDGSGLEEKVRAKLVPSQEETKLSVEESEAAGDGVDTKVAQGATEKSPEDKVQIAANEETQEREEQMKEGEETEGSEEDDKENDKTEEMPNDSVLENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLGEVSVESENYVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIENRMAVLNEQVKEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVPSGNEVSENMEEEAENQAESRAAVEGTVEAGATVESTAC
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P49321 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K20 | Acetylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| K33 | Acetylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| T123 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| R140 | Methylation | Uniprot | |
| Y148 | Phosphorylation | Uniprot | |
| K154 | Acetylation | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| K180 | Acetylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| T202 | Phosphorylation | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| T228 | Phosphorylation | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| T248 | Phosphorylation | Uniprot | |
| C254 | S-Nitrosylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| T291 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| T390 | Phosphorylation | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| S397 | Phosphorylation | Uniprot | |
| T402 | Phosphorylation | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| K417 | Acetylation | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| T425 | Phosphorylation | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| T448 | Phosphorylation | Uniprot | |
| K450 | Acetylation | Uniprot | |
| S451 | Phosphorylation | Uniprot | |
| K455 | Acetylation | Uniprot | |
| T464 | Phosphorylation | Uniprot | |
| T477 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| T490 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S503 | Phosphorylation | Uniprot | |
| T534 | Phosphorylation | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| Y540 | Phosphorylation | Uniprot | |
| S567 | Phosphorylation | Uniprot | |
| Y575 | Phosphorylation | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| R617 | Methylation | Uniprot | |
| K626 | Ubiquitination | Uniprot | |
| K636 | Sumoylation | Uniprot | |
| K643 | Acetylation | Uniprot | |
| K643 | Ubiquitination | Uniprot | |
| K652 | Ubiquitination | Uniprot | |
| K657 | Ubiquitination | Uniprot | |
| S659 | Phosphorylation | Uniprot | |
| S662 | Phosphorylation | Uniprot | |
| K671 | Ubiquitination | Uniprot | |
| T683 | Phosphorylation | Uniprot | |
| S689 | Phosphorylation | Uniprot | |
| S690 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | Uniprot | |
| K698 | Acetylation | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| T700 | Phosphorylation | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| S705 | Phosphorylation | Uniprot | |
| S706 | Phosphorylation | Uniprot | |
| S713 | Phosphorylation | Uniprot | |
| K721 | Methylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| K733 | Acetylation | Uniprot | |
| K734 | Acetylation | Uniprot | |
| K736 | Acetylation | Uniprot | |
| K736 | Sumoylation | Uniprot | |
| S745 | Phosphorylation | Uniprot | |
| S751 | Phosphorylation | Uniprot | |
| S756 | Phosphorylation | Uniprot | |
| C788 | S-Nitrosylation | Uniprot |
Research Backgrounds
Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.
Cytoplasm. Nucleus.
Isoform 1 is testis- and sperm-specific.
Binds to linker H1 histones but not to core histones (By similarity). Interacts with histones H2A, H2B, H3 and H4. Also binds to HSP90 in the cytoplasm. This interaction stimulates binding of NASP to HIST1H1T/H1T (By similarity).
Belongs to the NASP family.
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