MYH4 Antibody - #DF12165

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Product: MYH4 Antibody
Catalog: DF12165
Description: Rabbit polyclonal antibody to MYH4
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 230kd; 223kD(Calculated).
Uniprot: Q9Y623
RRID: AB_2844970

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
MYH4 Antibody detects endogenous levels of total MYH4.
RRID:
AB_2844970
Cite Format: Affinity Biosciences Cat# DF12165, RRID:AB_2844970.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

fetal; MYH2B; Myh4; MYH4_HUMAN; MyHC-2b; MyHC-IIb; Myosin heavy chain 2b; Myosin heavy chain 4; Myosin heavy chain 4 skeletal muscle; Myosin heavy chain; Myosin heavy chain IIb; Myosin heavy chain skeletal muscle fetal; Myosin-4; skeletal muscle;

Immunogens

Immunogen:
Uniprot:

Q9Y623(MYH4_HUMAN) >>Visit HPA database.

Gene(ID):
MYH4(4622)
Sequence:
MSSDSEMAIFGEAAPFLRKSEKERIEAQNKPFDAKTSVFVVDPKESYVKAIVQSREGGKVTAKTEAGATVTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEPASGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIEMLLITTNPYDFAFVSQGEITVPSIDDQEELMATDSAVDILGFTADEKVAIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKSLCYPRVKVGNEFVTKGQTVQQVYNAVGALAKAIYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKPEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLAFLFSGAQTAEAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIEIDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLAQLITRTQAICRGFLMRVEFRKMMERRESIFCIQYNIRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQMEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLCMDLERAKRKLEGDLKLAQESTMDTENDKQQLNEKLKKKEFEMSNLQGKIEDEQALAIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEESTLQHEATAAALRKKHADSVAELGEQIDSLQRVKQKLEKEKSELKMEINDLASNMETVSKAKANFEKMCRTLEDQLSEIKTKEEEQQRLINELSAQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELKRQLEEETKAKSTLAHALQSARHDCDLLREQYEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERSNAACIALDKKQRNFDKVLAEWKQKYEETQAELEASQKESRSLSTELFKVKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHEKSELQTSLEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEELDQLKRNHLRVVESMQSTLDAEIRSRNDALRIKKKMEGDLNEMEIQLNHANRQAAEALRNLRNTQGILKDTQLHLDDAIRGQDDLKEQLAMVERRANLMQAEVEELRASLERTERGRKMAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQKLEARVRELESEVESEQKHNVEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQTKVKAYKRQAEEAEEQSNVNLAKFRKLQHELEEAKERADIAESQVNKLRVKSREVHTKVISEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
92
Rabbit
92
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y623 As Substrate

Site PTM Type Enzyme
T64 Phosphorylation
T69 Phosphorylation
Y163 Phosphorylation
T168 Phosphorylation
Y195 Phosphorylation
T198 Phosphorylation
K249 Ubiquitination
T258 Phosphorylation
S263 Phosphorylation
Y269 Phosphorylation
K273 Ubiquitination
T356 Phosphorylation
Y362 Phosphorylation
T381 Phosphorylation
Y389 Phosphorylation
Y424 Phosphorylation
K505 Acetylation
Y556 Phosphorylation
T607 Phosphorylation
Y612 Phosphorylation
T664 Phosphorylation
Y719 Phosphorylation
S732 Phosphorylation
S742 Phosphorylation
K744 Acetylation
S746 Phosphorylation
T790 Phosphorylation
Y820 Phosphorylation
K857 Methylation
K862 Methylation
K922 Acetylation
S952 Phosphorylation
K955 Acetylation
K956 Acetylation
T964 Phosphorylation
T983 Phosphorylation
T992 Phosphorylation
T997 Phosphorylation
K1030 Methylation
S1041 Phosphorylation
K1046 Acetylation
K1087 Methylation
K1097 Methylation
K1111 Acetylation
K1113 Acetylation
R1130 Methylation
S1132 Phosphorylation
S1141 Phosphorylation
S1144 Phosphorylation
T1161 Phosphorylation
S1162 Phosphorylation
S1185 Phosphorylation
T1186 Phosphorylation
T1192 Phosphorylation
K1223 Ubiquitination
K1251 Ubiquitination
S1261 Phosphorylation
K1264 Ubiquitination
T1265 Phosphorylation
S1278 Phosphorylation
T1286 Phosphorylation
S1288 Phosphorylation
S1292 Phosphorylation
S1303 Phosphorylation
S1306 Phosphorylation
T1313 Phosphorylation
Y1351 Phosphorylation
S1366 Phosphorylation
S1370 Phosphorylation
T1377 Phosphorylation
Y1379 Phosphorylation
T1381 Phosphorylation
T1387 Phosphorylation
K1394 Acetylation
K1395 Acetylation
K1396 Acetylation
S1413 Phosphorylation
T1421 Phosphorylation
Y1464 Phosphorylation
T1467 Phosphorylation
S1478 Phosphorylation
S1480 Phosphorylation
S1482 Phosphorylation
T1483 Phosphorylation
S1514 Phosphorylation
T1517 Phosphorylation
S1574 Phosphorylation
S1600 Phosphorylation
S1603 Phosphorylation
T1604 Phosphorylation
S1714 Phosphorylation
T1722 Phosphorylation
T1725 Phosphorylation
S1726 Phosphorylation
T1730 Phosphorylation
K1731 Acetylation
S1739 Phosphorylation
T1764 Phosphorylation
K1774 Acetylation
K1788 Acetylation
K1795 Acetylation
K1813 Ubiquitination
K1814 Ubiquitination
T1855 Phosphorylation
Y1856 Phosphorylation
K1863 Acetylation
S1919 Phosphorylation

Research Backgrounds

Function:

Muscle contraction.

Subcellular Location:

Cytoplasm>Myofibril.
Note: Thick filaments of the myofibrils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Family&Domains:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

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