Flotillin 1 Antibody - #DF12155

(3)  
Product: Flotillin 1 Antibody
Catalog: DF12155
Description: Rabbit polyclonal antibody to Flotillin 1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 47 kDa; 47kD(Calculated).
Uniprot: O75955
RRID: AB_2844960

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
Flotillin 1 Antibody detects endogenous levels of total Flotillin 1.
RRID:
AB_2844960
Cite Format: Affinity Biosciences Cat# DF12155, RRID:AB_2844960.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLOT 1; FLOT1; FLOT1_HUMAN; Flotillin-1; Flotillin1; Integral membrane component of caveolae; Reggie 2;

Immunogens

Immunogen:
Uniprot:

O75955(FLOT1_HUMAN) >>Visit HPA database.

Gene(ID):
FLOT1(10211)
Sequence:
MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQVKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75955 As Substrate

Site PTM Type Enzyme
S19 Phosphorylation
K51 Ubiquitination
K54 Ubiquitination
Y56 Phosphorylation P12931 (SRC)
K78 Ubiquitination
K122 Ubiquitination
K126 Ubiquitination
S128 Phosphorylation
Y149 Phosphorylation P12931 (SRC)
K152 Ubiquitination
Y160 Phosphorylation P06241 (FYN)
S163 Phosphorylation
K166 Ubiquitination
K174 Ubiquitination
K184 Ubiquitination
K195 Ubiquitination
K198 Ubiquitination
Y203 Phosphorylation
S205 Phosphorylation
K211 Ubiquitination
Y216 Phosphorylation
K220 Methylation
K220 Ubiquitination
Y223 Phosphorylation
Y238 Phosphorylation
K244 Ubiquitination
Y292 Phosphorylation
K293 Ubiquitination
K302 Ubiquitination
S315 Phosphorylation
K341 Ubiquitination
Y348 Phosphorylation
S383 Phosphorylation
S385 Phosphorylation
T387 Phosphorylation
K392 Ubiquitination
S407 Phosphorylation
K422 Ubiquitination

Research Backgrounds

Function:

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.

Subcellular Location:

Cell membrane>Peripheral membrane protein. Endosome. Membrane>Caveola>Peripheral membrane protein. Melanosome. Membrane raft.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Membrane-associated protein of caveola (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 (By similarity). Interacts with ECPAS.

Family&Domains:

Belongs to the band 7/mec-2 family. Flotillin subfamily.

Research Fields

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

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