Cyclophilin B Antibody - #DF12151

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Product: Cyclophilin B Antibody
Catalog: DF12151
Description: Rabbit polyclonal antibody to Cyclophilin B
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 24 kDa; 24kD(Calculated).
Uniprot: P23284
RRID: AB_2844956

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
Cyclophilin B Antibody detects endogenous levels of total Cyclophilin B.
RRID:
AB_2844956
Cite Format: Affinity Biosciences Cat# DF12151, RRID:AB_2844956.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AA408962; AA553318; AI844835; Cphn 2; Cphn2; Cyclophilin B; Cyclophilin like protein; CyP 20b; CYP S1; CYP-S1; CYPB; EC 5.2.1.8; MGC14109; MGC2224; OI9; peptidyl prolyl cis trans isomerase B; Peptidyl prolyl cis trans isomerase B precursor; Peptidyl-prolyl cis-trans isomerase B; peptidylprolyl isomerase B (cyclophilin B); Peptidylprolyl isomerase B; PPIase; PPIase B; Ppib; PPIB_HUMAN; Rotamase; Rotamase B; S cyclophilin; S-cyclophilin; SCYLP;

Immunogens

Immunogen:
Uniprot:

P23284(PPIB_HUMAN) >>Visit HPA database.

Gene(ID):
PPIB(5479)
Sequence:
MLRLSERNMKVLLAAALIAGSVFFLLLPGPSAADEKKKGPKVTVKVYFDLRIGDEDVGRVIFGLFGKTVPKTVDNFVALATGEKGFGYKNSKFHRVIKDFMIQGGDFTRGDGTGGKSIYGERFPDENFKLKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Chicken
100
Pig
92
Horse
92
Bovine
92
Sheep
92
Xenopus
92
Zebrafish
92
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23284 As Substrate

Site PTM Type Enzyme
Y47 Phosphorylation
R51 Methylation
K67 Ubiquitination
T72 Phosphorylation
T81 Phosphorylation
K84 Ubiquitination
Y88 Phosphorylation
K98 Acetylation
K98 Ubiquitination
R109 Methylation
K116 Ubiquitination
R122 Methylation
K129 Methylation
K129 Ubiquitination
K131 Acetylation
K131 Ubiquitination
S139 Phosphorylation
T147 Phosphorylation
S150 Phosphorylation
K158 Ubiquitination
K165 Acetylation
K165 Ubiquitination
K171 Acetylation
S184 Phosphorylation
T185 Phosphorylation
T187 Phosphorylation
S189 Phosphorylation
C202 S-Nitrosylation
K209 Acetylation
K209 Ubiquitination
K215 Acetylation

Research Backgrounds

Function:

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.

Subcellular Location:

Endoplasmic reticulum lumen. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with DYM. Interacts with CALR, CLGN and CANX.

Family&Domains:

Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.

Restrictive clause

 

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For Research Use Only.
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