CAPZA1 Antibody - #DF12145

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Product: CAPZA1 Antibody
Catalog: DF12145
Description: Rabbit polyclonal antibody to CAPZA1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 33kd; 33kD(Calculated).
Uniprot: P52907
RRID: AB_2844950

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%)
Clonality:
Polyclonal
Specificity:
CAPZA1 Antibody detects endogenous levels of total CAPZA1.
RRID:
AB_2844950
Cite Format: Affinity Biosciences Cat# DF12145, RRID:AB_2844950.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cap Z; Cappa 1; Cappa1; Capping protein (actin filament) muscle Z line alpha 1; Capping protein alpha 1; Capping protein muscle Z line alpha 1; CapZ alpha 1; CapZ alpha-1; CAPZ; CAPZA 1; Capza1; CAZ 1; CAZ1; CAZA1_HUMAN; F actin capping protein alpha 1 subunit; F-actin-capping protein subunit alpha-1;

Immunogens

Immunogen:
Uniprot:

P52907(CAZA1_HUMAN) >>Visit HPA database.

Gene(ID):
CAPZA1(829)
Sequence:
MADFDDRVSDEEKVRIAAKFITHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNMDQFTPVKIEGYEDQVLITEHGDLGNSRFLDPRNKISFKFDHLRKEASDPQPEEADGGLKSWRESCDSALRAYVKDHYSNGFCTVYAKTIDGQQTIIACIESHQFQPKNFWNGRWRSEWKFTITPPTAQVVGVLKIQVHYYEDGNVQLVSHKDVQDSLTVSNEAQTAKEFIKIIENAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
86
Xenopus
67
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52907 As Substrate

Site PTM Type Enzyme
A2 Acetylation
R7 Methylation
S9 Phosphorylation P68400 (CSNK2A1)
K13 Ubiquitination
K19 Acetylation
K19 Ubiquitination
T63 Phosphorylation
Y70 Phosphorylation
K97 Acetylation
K97 Ubiquitination
K103 Ubiquitination
S106 Phosphorylation
K118 Ubiquitination
S123 Phosphorylation
K133 Ubiquitination
K166 Ubiquitination
K178 Ubiquitination
Y198 Phosphorylation
K210 Ubiquitination
S215 Phosphorylation
T224 Phosphorylation
K226 Ubiquitination
K230 Ubiquitination
Y239 Phosphorylation
Y247 Phosphorylation
K256 Methylation
K256 Ubiquitination
K268 Sumoylation
K268 Ubiquitination
K273 Acetylation
K273 Sumoylation
K273 Ubiquitination
Y277 Phosphorylation
K278 Acetylation
K278 Sumoylation
K278 Ubiquitination
K281 Ubiquitination

Research Backgrounds

Function:

F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions.

Subcellular Location:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer of an alpha and a beta subunit. Interacts with S100A (By similarity). Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASHC1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), WASHC2 (WASHC2A or WASHC2C), WASHC3, WASHC4 and WASHC5. Interacts with S100B. Interacts with SH3BP1; recruits CAPZA1 to forming cell junctions. Interacts with CD2AP. Directly interacts with CRACD; this interaction decreases binding to actin.

Family&Domains:

Belongs to the F-actin-capping protein alpha subunit family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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