ACSL4/FACL4 Antibody - #DF12141
Product: | ACSL4/FACL4 Antibody |
Catalog: | DF12141 |
Description: | Rabbit polyclonal antibody to ACSL4/FACL4 |
Application: | WB IHC IF/ICC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
Mol.Wt.: | 79 kDa,74 kDa; 79kD(Calculated). |
Uniprot: | O60488 |
RRID: | AB_2844946 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12141, RRID:AB_2844946.
Fold/Unfold
ACS 4; ACS4; ACSL 4; Acsl4; ACSL4_HUMAN; acyl CoA synthetase 4; Acyl CoA synthetase long chain family member 4; FACL 4; FACL4; Fatty acid Coenzyme A ligase; fatty acid Coenzyme A ligase long-chain 4; LACS 4; LACS4; Lignoceroyl CoA synthase; Long chain 4; long chain acyl CoA synthetase 4; long chain fatty acid CoA ligase 4; long chain fatty acid Coenzyme A ligase 4; Long-chain acyl-CoA synthetase 4; Long-chain-fatty-acid--CoA ligase 4; MRX63; MRX68;
Immunogens
- O60488 ACSL4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKLKLNVLTIILLPVHLLITIYSALIFIPWYFLTNAKKKNAMAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELRNHYLKDIERMYGGK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O60488 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S23 | Phosphorylation | Uniprot | |
Y31 | Phosphorylation | Uniprot | |
T34 | Phosphorylation | Uniprot | |
K47 | Ubiquitination | Uniprot | |
K49 | Ubiquitination | Uniprot | |
K54 | Ubiquitination | Uniprot | |
S57 | Phosphorylation | Uniprot | |
T63 | Phosphorylation | Uniprot | |
K89 | Acetylation | Uniprot | |
K89 | Ubiquitination | Uniprot | |
K92 | Acetylation | Uniprot | |
S95 | Phosphorylation | Uniprot | |
K113 | Ubiquitination | Uniprot | |
K117 | Ubiquitination | Uniprot | |
S140 | Phosphorylation | Uniprot | |
T143 | Phosphorylation | Uniprot | |
K148 | Ubiquitination | Uniprot | |
K150 | Ubiquitination | Uniprot | |
K211 | Ubiquitination | Uniprot | |
C221 | S-Nitrosylation | Uniprot | |
K223 | Ubiquitination | Uniprot | |
Y227 | Phosphorylation | Uniprot | |
K231 | Ubiquitination | Uniprot | |
K312 | Ubiquitination | Uniprot | |
S352 | Phosphorylation | Uniprot | |
S353 | Phosphorylation | Uniprot | |
K354 | Ubiquitination | Uniprot | |
K356 | Ubiquitination | Uniprot | |
K360 | Ubiquitination | Uniprot | |
K367 | Ubiquitination | Uniprot | |
K383 | Ubiquitination | Uniprot | |
K388 | Ubiquitination | Uniprot | |
K397 | Acetylation | Uniprot | |
K397 | Ubiquitination | Uniprot | |
K401 | Acetylation | Uniprot | |
K401 | Ubiquitination | Uniprot | |
Y404 | Phosphorylation | Uniprot | |
K407 | Ubiquitination | Uniprot | |
K413 | Ubiquitination | Uniprot | |
Y415 | Phosphorylation | Uniprot | |
K426 | Ubiquitination | Uniprot | |
S447 | Phosphorylation | Uniprot | |
Y483 | Phosphorylation | Uniprot | |
T485 | Phosphorylation | Uniprot | |
K498 | Ubiquitination | Uniprot | |
K500 | Ubiquitination | Uniprot | |
T508 | Phosphorylation | Uniprot | |
K512 | Ubiquitination | Uniprot | |
K536 | Ubiquitination | Uniprot | |
Y541 | Phosphorylation | Uniprot | |
Y582 | Phosphorylation | Uniprot | |
S584 | Phosphorylation | Uniprot | |
K587 | Ubiquitination | Uniprot | |
K593 | Ubiquitination | Uniprot | |
S607 | Phosphorylation | Uniprot | |
K621 | Ubiquitination | Uniprot | |
K651 | Ubiquitination | Uniprot | |
K661 | Ubiquitination | Uniprot | |
K670 | Ubiquitination | Uniprot | |
S674 | Phosphorylation | Uniprot | |
T679 | Phosphorylation | Uniprot | |
T682 | Phosphorylation | Uniprot | |
T686 | Phosphorylation | Uniprot | |
K690 | Acetylation | Uniprot | |
K690 | Ubiquitination | Uniprot | |
K702 | Ubiquitination | Uniprot |
Research Backgrounds
Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion.
Mitochondrion outer membrane>Single-pass type III membrane protein. Peroxisome membrane>Single-pass type III membrane protein. Microsome membrane>Single-pass type III membrane protein. Endoplasmic reticulum membrane>Single-pass type III membrane protein. Cell membrane.
Belongs to the ATP-dependent AMP-binding enzyme family.
Research Fields
· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)
· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)
· Metabolism > Lipid metabolism > Fatty acid biosynthesis.
· Metabolism > Lipid metabolism > Fatty acid degradation.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Fatty acid metabolism.
· Organismal Systems > Endocrine system > PPAR signaling pathway.
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
References
Application: WB Species: Rat Sample: IEC-6 cells and Caco-2 cells
Application: IHC Species: Mouse Sample: colonic tissues
Application: WB Species: Rat Sample: spinal cord
Application: WB Species: Rat Sample:
Application: WB Species: Rat Sample: H9c2 cells
Application: WB Species: rat Sample: H9c2 cells
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