ACSL4/FACL4 Antibody | Affinity Biosciences

Product:	ACSL4/FACL4 Antibody
Catalog:	DF12141
Description:	Rabbit polyclonal antibody to ACSL4/FACL4
Application:	WB IHC IF/ICC
Cited expt.:	WB, IHC, IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	79 kDa,74 kDa; 79kD(Calculated).
Uniprot:	O60488
RRID:	AB_2844946

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

ACSL4/FACL4 Antibody detects endogenous levels of total ACSL4/FACL4.

RRID:

AB_2844946
Cite Format: Affinity Biosciences Cat# DF12141, RRID:AB_2844946.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACS 4; ACS4; ACSL 4; Acsl4; ACSL4_HUMAN; acyl CoA synthetase 4; Acyl CoA synthetase long chain family member 4; FACL 4; FACL4; Fatty acid Coenzyme A ligase; fatty acid Coenzyme A ligase long-chain 4; LACS 4; LACS4; Lignoceroyl CoA synthase; Long chain 4; long chain acyl CoA synthetase 4; long chain fatty acid CoA ligase 4; long chain fatty acid Coenzyme A ligase 4; Long-chain acyl-CoA synthetase 4; Long-chain-fatty-acid--CoA ligase 4; MRX63; MRX68;

Immunogens

Immunogen:

A synthesized peptide derived from human ACSL4/FACL4, corresponding to a region within N-terminal amino acids.

Uniprot:

O60488(ACSL4_HUMAN) >>Visit HPA database.

Gene(ID):

ACSL4(2182)

Sequence:

O60488 ACSL4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKLKLNVLTIILLPVHLLITIYSALIFIPWYFLTNAKKKNAMAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELRNHYLKDIERMYGGK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

O60488_ACSL4_HUMAN

Function:

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion.

Subcellular Location:

Mitochondrion outer membrane>Single-pass type III membrane protein. Peroxisome membrane>Single-pass type III membrane protein. Microsome membrane>Single-pass type III membrane protein. Endoplasmic reticulum membrane>Single-pass type III membrane protein. Cell membrane.

Family&Domains:

Belongs to the ATP-dependent AMP-binding enzyme family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)

· Metabolism > Lipid metabolism > Fatty acid biosynthesis.

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

References

1). Identification and analysis of microplastic aggregation in CAR-T cells. Journal of hazardous materials, 2024 (PubMed: 39488976) [IF=13.6]

2). Internalized polystyrene nanoplastics trigger testicular damage and promote ferroptosis via CISD1 downregulation in mouse spermatocyte. JOURNAL OF NANOBIOTECHNOLOGY, 2025 [IF=12.6]

Application: WB Species: Mouse Sample:

Fig. 2 PS-NPs induce testicular ferroptosis in mice. (A-C) The concentration of total Fe, MDA and GSH in mice serum after PS-NPs exposure. (D-F) Effect of PS-NPs on total Fe, MDA and GSH in mice testes. (G) Gene expression of ferroptosis-associated genes in isolated testes using qRT-PCR after treatment. (H and I) Western blotting and quantitative analysis of ferroptosis-related protein expression. (J and K) Immunofluorescence images of GPX4 and SLC7A11 in testes following PS-NPs treatment for 35 days

3). Interplay between lipid dysregulation and ferroptosis in chondrocytes and the targeted therapy effect of metformin on osteoarthritis. Journal of advanced research, 2025 (PubMed: 38621621) [IF=11.4]

4). The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3. Redox biology, 2023 (PubMed: 37099926) [IF=10.7]

5). Dual-responsive stem cell microspheres modified with BDNF for enhanced neural repair in diabetic erectile dysfunction. Journal of controlled release : official journal of the Controlled Release Society, 2025 (PubMed: 39761859) [IF=10.5]

6). EBF1-induced CSRP2 boosts the progression of B-cell acute lymphocytic leukemia by inhibiting ferroptosis. Cancer letters, 2025 (PubMed: 39952599) [IF=9.1]

7). Selenium nanoparticles alleviate renal ischemia/reperfusion injury by inhibiting ferritinophagy via the XBP1/NCOA4 pathway. Cell communication and signaling : CCS, 2024 (PubMed: 39061070) [IF=8.4]

Application: IF/ICC Species: Human Sample:

Fig. 1. Renal tubular epithelial cells undergo ferroptosis in I/R-AKI. (A) Single-cell sequencing identified clusters in both sham-operated and I/R-operated kidneys at day 3 post-surgery, depicted in a t-distributed stochastic neighbor embedding (tSNE) map. PT, proximal tubule; DT, distal convoluted tubule; LOH, ascending loop of Henle; ECs, endothelial cells. (B) Average expression levels of mouse single cell type-specific genes are shown in pheatmap. Mean expression values of the genes were calculated in each cluster. The full list of cell types and genes is shown in Additional file1: Table S1. (C-E) Subtypes of PT with differentially expressed genes are illustrated in a tSNE plot (C), and the representative marker genes across the 3 subtypes of PT are depicted in pheatmap (D) and VlnPlot (E). (F-G) The expression levels of ferroptosis-associated genes Gpx4 and Acsl4 in the subtypes of PT were identified by FeaturePlot (F) and Dotplot (G). (H-I) Western blot (H) and statistical analysis (I) of the expression levels of GPX4 and SLC7A11 proteins in the renal cortex of sham-operated and I/R-operated mice post-surgery. ***p

8). The involvement of the Stat1/Nrf2 pathway in exacerbating Crizotinib-induced liver injury: implications for ferroptosis. Cell death & disease, 2024 (PubMed: 39160159) [IF=8.1]

9). Human umbilical cord mesenchymal stem cells ameliorate erectile dysfunction in rats with diabetes mellitus through the attenuation of ferroptosis. Stem Cell Research & Therapy, 2022 (PubMed: 36064453) [IF=7.5]

10). Med1 inhibits ferroptosis and alleviates liver injury in acute liver failure via Nrf2 activation. Cell & bioscience, 2024 (PubMed: 38678227) [IF=7.5]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ACSL4/FACL4 Antibody - #DF12141