TIP47 Antibody - #DF12139

(1)   (22)  
Product: TIP47 Antibody
Catalog: DF12139
Description: Rabbit polyclonal antibody to TIP47
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 47 kDa; 47kD(Calculated).
Uniprot: O60664
RRID: AB_2844944

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
TIP47 Antibody detects endogenous levels of total TIP47.
RRID:
AB_2844944
Cite Format: Affinity Biosciences Cat# DF12139, RRID:AB_2844944.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR binding protein; 47 kDa MPR-binding protein; Cargo selection protein TIP47; M6PRBP 1; M6PRBP1; Mannose 6 phosphate receptor binding protein 1; Mannose-6-phosphate receptor-binding protein 1; MGC11117; MGC2012; MPR binding protein 47 kDa; Perilipin 3; Perilipin-3; Perilipin3; Placental protein 17; PLIN3; PLIN3_HUMAN; pp17; Tail interacting protein 47 kD; TIP47;

Immunogens

Immunogen:
Uniprot:

O60664(PLIN3_HUMAN) >>Visit HPA database.

Gene(ID):
PLIN3(10226)
Sequence:
MSADGAEADGSTQVTVEEPVQQPSVVDRVASMPLISSTCDMVSAAYASTKESYPHIKTVCDAAEKGVRTLTAAAVSGAQPILSKLEPQIASASEYAHRGLDKLEENLPILQQPTEKVLADTKELVSSKVSGAQEMVSSAKDTVATQLSEAVDATRGAVQSGVDKTKSVVTGGVQSVMGSRLGQMVLSGVDTVLGKSEEWADNHLPLTDAELARIATSLDGFDVASVQQQRQEQSYFVRLGSLSERLRQHAYEHSLGKLRATKQRAQEALLQLSQVLSLMETVKQGVDQKLVEGQEKLHQMWLSWNQKQLQGPEKEPPKPEQVESRALTMFRDIAQQLQATCTSLGSSIQGLPTNVKDQVQQARRQVEDLQATFSSIHSFQDLSSSILAQSRERVASAREALDHMVEYVAQNTPVTWLVGPFAPGITEKAPEEKK

PTMs - O60664 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S31 Phosphorylation
S36 Phosphorylation
Y46 Phosphorylation
K65 Acetylation
K65 Ubiquitination
S83 Phosphorylation
K84 Acetylation
K84 Ubiquitination
S91 Phosphorylation
S93 Phosphorylation
Y95 Phosphorylation
K102 Ubiquitination
K116 Ubiquitination
K122 Acetylation
K122 Sumoylation
K122 Ubiquitination
K128 Ubiquitination
S130 Phosphorylation
K140 Ubiquitination
S148 Phosphorylation
T154 Phosphorylation
S160 Phosphorylation
K164 Acetylation
K164 Ubiquitination
T165 Phosphorylation
S167 Phosphorylation
T170 Phosphorylation
S175 Phosphorylation
S179 Phosphorylation
S187 Phosphorylation
K195 Ubiquitination
S196 Phosphorylation
T207 Phosphorylation
T216 Phosphorylation
S217 Phosphorylation
S241 Phosphorylation
S243 Phosphorylation
R245 Methylation
K283 Ubiquitination
K289 Ubiquitination
S303 Phosphorylation
K307 Ubiquitination
K314 Ubiquitination
T342 Phosphorylation
S347 Phosphorylation
K356 Ubiquitination
T372 Phosphorylation
S374 Phosphorylation
S378 Phosphorylation
S390 Phosphorylation
T412 Phosphorylation
T415 Phosphorylation
K428 Ubiquitination

Research Backgrounds

Function:

Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.

Subcellular Location:

Cytoplasm. Endosome membrane>Peripheral membrane protein>Cytoplasmic side. Lipid droplet.
Note: Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homooligomer. Interacts with M6PR (via the cytoplasmic domain). Interacts with IGF2R (via the cytoplasmic domain). Isoform 2 may exist as a homodimer (known as PP17C).

Family&Domains:

Belongs to the perilipin family.

References

1). Interface-Targeting Strategy Enables Two-Photon Fluorescent Lipid Droplet Probes for High-Fidelity Imaging of Turbid Tissues and Detecting Fatty Liver. ACS Applied Materials & Interfaces, 2018 (PubMed: 29521495) [IF=9.5]

Application: IF/ICC    Species:    Sample: HeLa cells

Figure S24|Confocal fluorescence images of HeLa cells co@stained with N@Cy and anti@TIP47 antiserum. (1): DIC; (2): N-Cy; (4): Anti@TIP47 antiserum; (4): Merged image of (2) and (3); (5): Merged image of (1) @ (3). (6): The normalized fluorescence profiles of N-Cy and ER red along the white line in (4).N-Cy: 4 UM, 2 min; λex = 488 nm, λem = 510@540 nm. Anti@TIP47 antiserum (fluorescence from Alexa Fluor 594@conjugated goat anti@rabbit IgG): λex = 561 nm; λem = 600@700 nm. Bar = 20 Um.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.