Estrogen Sulfotransferase Antibody - #DF12137

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Product: Estrogen Sulfotransferase Antibody
Catalog: DF12137
Description: Rabbit polyclonal antibody to Estrogen Sulfotransferase
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit
Mol.Wt.: 32-35 kDa; 35kD(Calculated).
Uniprot: P49888
RRID: AB_2844942

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Horse(100%), Rabbit(89%)
Clonality:
Polyclonal
Specificity:
Estrogen Sulfotransferase Antibody detects endogenous levels of total Estrogen Sulfotransferase.
RRID:
AB_2844942
Cite Format: Affinity Biosciences Cat# DF12137, RRID:AB_2844942.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EST; EST-1; EST1; Estrogen sulfotransferase; estrogen-preferring; estrone sulfotransferase; ST1E1; ST1E1_HUMAN; STE; Sulfotransferase 1E1; Sulfotransferase; Sulfotransferase estrogen preferring; SULT1E1;

Immunogens

Immunogen:
Uniprot:

P49888(ST1E1_HUMAN) >>Visit HPA database.

Gene(ID):
SULT1E1(6783)
Expression:
P49888 ST1E1_HUMAN:

Liver, intestine and at lower level in the kidney.

Sequence:
MNSELDYYEKFEEVHGILMYKDFVKYWDNVEAFQARPDDLVIATYPKSGTTWVSEIVYMIYKEGDVEKCKEDVIFNRIPFLECRKENLMNGVKQLDEMNSPRIVKTHLPPELLPASFWEKDCKIIYLCRNAKDVAVSFYYFFLMVAGHPNPGSFPEFVEKFMQGQVPYGSWYKHVKSWWEKGKSPRVLFLFYEDLKEDIRKEVIKLIHFLERKPSEELVDRIIHHTSFQEMKNNPSTNYTTLPDEIMNQKLSPFMRKGITGDWKNHFTVALNEKFDKHYEQQMKESTLKFRTEI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
89
Rabbit
89
Bovine
78
Xenopus
67
Chicken
67
Sheep
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49888 As Substrate

Site PTM Type Enzyme
Y192 Phosphorylation
K205 Acetylation

Research Backgrounds

Function:

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency. Does not sulfonate cortisol, testosterone and dopamine.

Subcellular Location:

Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Liver, intestine and at lower level in the kidney.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the sulfotransferase 1 family.

Research Fields

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

Restrictive clause

 

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