SEC61G Antibody - #DF12136

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Product: SEC61G Antibody
Catalog: DF12136
Description: Rabbit polyclonal antibody to SEC61G
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 7.7 kDa; 8kD(Calculated).
Uniprot: P60059
RRID: AB_2844941

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SEC61G Antibody detects endogenous levels of total SEC61G.
RRID:
AB_2844941
Cite Format: Affinity Biosciences Cat# DF12136, RRID:AB_2844941.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Protein transport protein Sec61 subunit gamma; SC61G_HUMAN; Sec61 gamma subunit; SEC61G; SSS1;

Immunogens

Immunogen:
Uniprot:

P60059(SC61G_HUMAN) >>Visit HPA database.

Gene(ID):
SEC61G(23480)
Sequence:
MDQVMQFVEPSRQFVKDSIRLVKRCTKPDRKEFQKIAMATAIGFAIMGFIGFFVKLIHIPINNIIVGG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P60059 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K16 Ubiquitination
S18 Phosphorylation

Research Backgrounds

Function:

Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across endoplasmic reticulum (ER).

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

The ER translocon complex consists of channel-forming core components SEC61A1, SEC61B and SEC61G and different auxiliary components such as SEC62 and SEC63.

Family&Domains:

Belongs to the SecE/SEC61-gamma family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein export.

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

References

1). Identification of dihydrotanshinone I as an ERp57 inhibitor with anti-breast cancer properties via the UPR pathway. BIOCHEMICAL PHARMACOLOGY, 2021 (PubMed: 34062127) [IF=5.8]

Application: WB    Species: Human    Sample: breast cancer cell

Fig. 5. DHT activated ER stress and UPR signaling pathways. MDA-MB-231 cells were incubated with 3 μ M DHT for the indicated time. (A) Western blot analysis was performed with antibodies against Sec61γ, BiP, p-PERK/PERK, p-eIF2 α /eIF2 α , and ATF4. The quantitative densitometric analysis of Sec61γ (B), BiP (C), p-PERK/ PERK (D), p-eIF2 α /eIF2 α (E), and ATF4 (F) was carried out. A representative Western blot is shown (n = 3). *P < 0.05 vs 0 h treatment time point.

Application: WB    Species: human    Sample: MDA-MB-231 cells

Fig. 5.| DHT activated ER stress and UPR signaling pathways. MDA-MB-231 cells were incubated with 3 μM DHT for the indicated time. (A) Western blot analysis was performed with antibodies against Sec61γ, BiP, p-PERK/PERK, p-eIF2α/eIF2α, and ATF4. The quantitative densitometric analysis of Sec61γ (B), BiP (C), p-PERK/PERK (D), p-eIF2α/eIF2α (E), and ATF4 (F) was carried out.
2). SEC61G overexpression and DNA amplification correlates with prognosis and immune cell infiltration in head and neck squamous cell carcinoma. Cancer Medicine, 2021 (PubMed: 34590792) [IF=4.0]

Application: IHC    Species: Human    Sample: oropharyngeal cancer

FIGURE 6 The expression and prognostic values of SEC61G in oropharyngeal cancer: (A, B) representative IHC staining intensities of SEC61G in normal tissues: SEC61G was scored as (A) negative and (B) weak; (C–F) representative IHC staining patterns of SEC61G in OPC tissues: (C) negative, (D) weak, (E) moderate and (F) strong; (G, H) the proportions of SEC61G in normal and tumor tissues; (I) Overall survival rate of SEC61G in oropharyngeal cancer; (J) Progression‐free survival of SEC61G in oropharyngeal cancer

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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