RAB3D Antibody - #DF12135

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Product: RAB3D Antibody
Catalog: DF12135
Description: Rabbit polyclonal antibody to RAB3D
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 28 kDa; 24kD(Calculated).
Uniprot: O95716
RRID: AB_2844940

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
RAB3D Antibody detects endogenous levels of total RAB3D.
RRID:
AB_2844940
Cite Format: Affinity Biosciences Cat# DF12135, RRID:AB_2844940.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

D2-2; Glioblastoma overexpressed; GOV; RAB 16; RAB16; Rab3d; Rab3D upregulated with myeloid differentiation; RAB3D, member RAS oncogene family; RAB3D_HUMAN; RAD3D; Ras-related protein Rab-3D;

Immunogens

Immunogen:
Uniprot:

O95716(RAB3D_HUMAN) >>Visit HPA database.

Gene(ID):
RAB3D(9545)
Expression:
O95716 RAB3D_HUMAN:

Highly expressed in granulocytes of peripheral blood. Constitutively expressed at low levels in all hematopoietic cell lines investigated.

Sequence:
MASAGDTQAGPRDAADQNFDYMFKLLLIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDKRIKLQIWDTAGQERYRTITTAYYRGAMGFLLMYDIANQESFAAVQDWATQIKTYSWDNAQVILVGNKCDLEDERVVPAEDGRRLADDLGFEFFEASAKENINVKQVFERLVDVICEKMNESLEPSSSSGSNGKGPAVGDAPAPQPSSCSC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
92
Bovine
92
Sheep
92
Xenopus
75
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95716 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S3 Phosphorylation
Y21 Phosphorylation
T78 Phosphorylation
T88 Phosphorylation
Y91 Phosphorylation

Research Backgrounds

Function:

Protein transport. Probably involved in regulated exocytosis (By similarity).

PTMs:

Phosphorylation of Thr-86 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM and CHML.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in granulocytes of peripheral blood. Constitutively expressed at low levels in all hematopoietic cell lines investigated.

Subunit Structure:

Interacts with RIMS1, RIMS2, RPH3A, RPH3AL and RAB3IP (By similarity). Interacts with CHM and CHML; phosphorylation at Thr-86 disrupts these interactions.

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

Research Fields

· Organismal Systems > Digestive system > Pancreatic secretion.

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