PYGL Antibody | Affinity Biosciences

Product:	PYGL Antibody
Catalog:	DF12134
Description:	Rabbit polyclonal antibody to PYGL
Application:	WB IHC
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	97 kDa; 97kD(Calculated).
Uniprot:	P06737
RRID:	AB_2844939

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Zebrafish(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

PYGL Antibody detects endogenous levels of total PYGL.

RRID:

AB_2844939
Cite Format: Affinity Biosciences Cat# DF12134, RRID:AB_2844939.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Glycogen phosphorylase; Glycogen phosphorylase liver; Glycogen phosphorylase liver form; GSD6; Hers disease, glycogen storage disease type VI; liver form; OTTHUMP00000233649; OTTHUMP00000233651; Phosphorylase glycogen liver; Pygl; PYGL_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human PYGL, corresponding to a region within the internal amino acids.

Uniprot:

P06737(PYGL_HUMAN) >>Visit HPA database.

Gene(ID):

PYGL(5836)

Sequence:

P06737 PYGL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAKPLTDQEKRRQISIRGIVGVENVAELKKSFNRHLHFTLVKDRNVATTRDYYFALAHTVRDHLVGRWIRTQQHYYDKCPKRVYYLSLEFYMGRTLQNTMINLGLQNACDEAIYQLGLDIEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEYGIFNQKIRDGWQVEEADDWLRYGNPWEKSRPEFMLPVHFYGKVEHTNTGTKWIDTQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLRDFNVGDYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDLVEKLLPRHLEIIYEINQKHLDRIVALFPKDVDRLRRMSLIEEEGSKRINMAHLCIVGSHAVNGVAKIHSDIVKTKVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKIGEDYVKDLSQLTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDVQVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAKMIIKLITSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFKDIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWNTMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNESNKVNGN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Pig

Bovine

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P06737_PYGL_HUMAN

Function:

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

PTMs:

Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.

Family&Domains:

Belongs to the glycogen phosphorylase family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

References

1). Tributyltin exposure disturbs hepatic glucose metabolism in male mice. TOXICOLOGY, 2019 (PubMed: 31306684) [IF=4.8]

Application: WB Species: mouse Sample: liver

Fig. 5. |Effect of tributyltin (TBT) on hepatic glucose metabolism in male mice after exposure for 45 days. (A) Representative chemiluminescence detection of protein expression in liver.

2). Analysis of the expression, function and signaling of glycogen phosphorylase isoforms in hepatocellular carcinoma. Oncology Letters, 2022 (PubMed: 35761940) [IF=2.5]

Application: WB Species: Human Sample: HCC cell

Figure 1. Analysis of the expression of PYGB, PYGL and PYGM in HCC. (A) Analysis of PYGB, PYGL and PYGM mRNA expression in HCC from UALCAN (http://ualcan.path.uab.edu/analysis.html). Dataset TCGA-LIHC from study accession phs000178.v11.p8 was used. (B) Analysis of PYGB, PYGL and PYGM protein expression in a human HCC cell line (MHCC97H) by western blotting. (C) Promoter methylation level analysis of PYGB, PYGL and PYGM in HCC from UALCAN. The dataset TCGA-LIHC from study accession phs000178.v11.p8 was used. *P

3). Exposure of zebrafish to a cold environment triggered cellular autophagy in zebrafish liver. JOURNAL OF FISH DISEASES, 2022 (PubMed: 35395109) [IF=2.2]

4). Biochemical analysis challenging Western blot analysis as validation step for antibodies intended for ELISA and Immunohistochemistry use. bioRxiv, 2022

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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PYGL Antibody - #DF12134