PSAT1 Antibody - #DF12132

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Product: PSAT1 Antibody
Catalog: DF12132
Description: Rabbit polyclonal antibody to PSAT1
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 37-40 kDa; 40kD(Calculated).
Uniprot: Q9Y617
RRID: AB_2844937

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
PSAT1 Antibody detects endogenous levels of total PSAT1.
RRID:
AB_2844937
Cite Format: Affinity Biosciences Cat# DF12132, RRID:AB_2844937.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 2.6.1.52; Endometrial progesterone induced protein; EPIP; MGC1460; NLS2; Phosphohydroxythreonine aminotransferase; phosphoserine aminotransferase 1; Phosphoserine aminotransferase; PSA; PSAT; Psat1; PSATD; SERC_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q9Y617(SERC_HUMAN) >>Visit HPA database.

Gene(ID):
PSAT1(29968)
Expression:
Q9Y617 SERC_HUMAN:

Expressed at high levels in the brain, liver, kidney and pancreas, and very weakly expressed in the thymus, prostate, testis and colon.

Sequence:
MDAPRQVVNFGPGPAKLPHSVLLEIQKELLDYKGVGISVLEMSHRSSDFAKIINNTENLVRELLAVPDNYKVIFLQGGGCGQFSAVPLNLIGLKAGRCADYVVTGAWSAKAAEEAKKFGTINIVHPKLGSYTKIPDPSTWNLNPDASYVYYCANETVHGVEFDFIPDVKGAVLVCDMSSNFLSKPVDVSKFGVIFAGAQKNVGSAGVTVVIVRDDLLGFALRECPSVLEYKVQAGNSSLYNTPPCFSIYVMGLVLEWIKNNGGAAAMEKLSSIKSQTIYEIIDNSQGFYVCPVEPQNRSKMNIPFRIGNAKGDDALEKRFLDKALELNMLSLKGHRSVGGIRASLYNAVTIEDVQKLAAFMKKFLEMHQL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
83
Zebrafish
83
Chicken
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y617 As Substrate

Site PTM Type Enzyme
M1 Acetylation
R5 Methylation
K16 Ubiquitination
S20 Phosphorylation
K27 Ubiquitination
Y32 Phosphorylation
K33 Ubiquitination
S38 Phosphorylation
R45 Methylation
K51 Acetylation
K51 Ubiquitination
K71 Ubiquitination
K94 Ubiquitination
R97 Methylation
C98 S-Nitrosylation
K110 Acetylation
K110 Ubiquitination
K117 Ubiquitination
K127 Acetylation
K127 Ubiquitination
K184 Ubiquitination
K190 Ubiquitination
R222 Methylation
S226 Phosphorylation
K269 Acetylation
K269 Ubiquitination
K274 Ubiquitination
Y279 Phosphorylation
K300 Ubiquitination
K311 Acetylation
K311 Ubiquitination
K318 Acetylation
K318 Ubiquitination
K323 Acetylation
K323 Ubiquitination
S331 Phosphorylation
K333 Acetylation
K333 Methylation
K333 Ubiquitination
R336 Methylation
S344 Phosphorylation
Y346 Phosphorylation
K356 Ubiquitination
K363 Ubiquitination

Research Backgrounds

Function:

Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.

Tissue Specificity:

Expressed at high levels in the brain, liver, kidney and pancreas, and very weakly expressed in the thymus, prostate, testis and colon.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.

Research Fields

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Vitamin B6 metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

 

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