PPAT Antibody | Affinity Biosciences

1/3

Western blot analysis of extracts from various samples, using PPAT Antibody.

DF12131 at 1/100 staining Mouse kidney tissue by IHC-P.

DF12131 at 1/100 staining Mouse thymus tissue by IHC-P.

Product:	PPAT Antibody
Catalog:	DF12131
Description:	Rabbit polyclonal antibody to PPAT
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	50-56kd; 57kD(Calculated).
Uniprot:	Q06203
RRID:	AB_2844936

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(91%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

PPAT Antibody detects endogenous levels of total PPAT.

RRID:

AB_2844936
Cite Format: Affinity Biosciences Cat# DF12131, RRID:AB_2844936.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Amidophosphoribosyltransferase [Precursor]; Amidophosphoribosyltransferase; ATase; Glutamine phosphoribosylpyrophosphatate amidotransferase; Glutamine phosphoribosylpyrophosphate amidotransferase; Glutamine PRPP amidotransferase; GPAT; Phosphoribosyl pyrophosphate amidotransferase; Ppat; PRAT; PUR1_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q06203(PUR1_HUMAN) >>Visit HPA database.

Gene(ID):

PPAT(5471)

Expression:

Q06203 PUR1_HUMAN:

Ubiquitously expressed.

Sequence:

Q06203 PUR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MELEELGIREECGVFGCIASGEWPTQLDVPHVITLGLVGLQHRGQESAGIVTSDGSSVPTFKSHKGMGLVNHVFTEDNLKKLYVSNLGIGHTRYATTGKCELENCQPFVVETLHGKIAVAHNGELVNAARLRKKLLRHGIGLSTSSDSEMITQLLAYTPPQEQDDTPDWVARIKNLMKEAPTAYSLLIMHRDVIYAVRDPYGNRPLCIGRLIPVSDINDKEKKTSETEGWVVSSESCSFLSIGARYYREVLPGEIVEISRHNVQTLDIISRSEGNPVAFCIFEYVYFARPDSMFEDQMVYTVRYRCGQQLAIEAPVDADLVSTVPESATPAALAYAGKCGLPYVEVLCKNRYVGRTFIQPNMRLRQLGVAKKFGVLSDNFKGKRIVLVDDSIVRGNTISPIIKLLKESGAKEVHIRVASPPIKYPCFMGINIPTKEELIANKPEFDHLAEYLGANSVVYLSVEGLVSSVQEGIKFKKQKEKKHDIMIQENGNGLECFEKSGHCTACLTGKYPVELEW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Chicken

Zebrafish

Bovine

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q06203 As Substrate

Q06203

Site	PTM Type	Source
M1	Acetylation	Uniprot
T52	Phosphorylation	Uniprot
K62	Acetylation	Uniprot
K62	Methylation	Uniprot
K62	Ubiquitination	Uniprot
K65	Ubiquitination	Uniprot
K80	Ubiquitination	Uniprot
K81	Acetylation	Uniprot
K81	Ubiquitination	Uniprot
Y94	Phosphorylation	Uniprot
K99	Acetylation	Uniprot
K99	Ubiquitination	Uniprot
R130	Methylation	Uniprot
T166	Phosphorylation	Uniprot
K178	Ubiquitination	Uniprot
K220	Ubiquitination	Uniprot
C339	S-Nitrosylation	Uniprot
C348	S-Nitrosylation	Uniprot
K349	Ubiquitination	Uniprot
T356	Phosphorylation	Uniprot
K371	Acetylation	Uniprot
K372	Acetylation	Uniprot
K372	Ubiquitination	Uniprot
K381	Ubiquitination	Uniprot
K383	Methylation	Uniprot
S399	Phosphorylation	Uniprot
K403	Ubiquitination	Uniprot
K406	Ubiquitination	Uniprot
S408	Phosphorylation	Uniprot
K411	Ubiquitination	Uniprot
K482	Acetylation	Uniprot
K499	Acetylation	Uniprot
K510	Ubiquitination	Uniprot

Research Backgrounds

Q06203_PUR1_HUMAN

Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Homotetramer.

Family&Domains:

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PPAT Antibody - #DF12131