Product: PPAT Antibody
Catalog: DF12131
Description: Rabbit polyclonal antibody to PPAT
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 50-56kd; 57kD(Calculated).
Uniprot: Q06203
RRID: AB_2844936

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(91%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
PPAT Antibody detects endogenous levels of total PPAT.
RRID:
AB_2844936
Cite Format: Affinity Biosciences Cat# DF12131, RRID:AB_2844936.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Amidophosphoribosyltransferase [Precursor]; Amidophosphoribosyltransferase; ATase; Glutamine phosphoribosylpyrophosphatate amidotransferase; Glutamine phosphoribosylpyrophosphate amidotransferase; Glutamine PRPP amidotransferase; GPAT; Phosphoribosyl pyrophosphate amidotransferase; Ppat; PRAT; PUR1_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q06203 PUR1_HUMAN:

Ubiquitously expressed.

Sequence:
MELEELGIREECGVFGCIASGEWPTQLDVPHVITLGLVGLQHRGQESAGIVTSDGSSVPTFKSHKGMGLVNHVFTEDNLKKLYVSNLGIGHTRYATTGKCELENCQPFVVETLHGKIAVAHNGELVNAARLRKKLLRHGIGLSTSSDSEMITQLLAYTPPQEQDDTPDWVARIKNLMKEAPTAYSLLIMHRDVIYAVRDPYGNRPLCIGRLIPVSDINDKEKKTSETEGWVVSSESCSFLSIGARYYREVLPGEIVEISRHNVQTLDIISRSEGNPVAFCIFEYVYFARPDSMFEDQMVYTVRYRCGQQLAIEAPVDADLVSTVPESATPAALAYAGKCGLPYVEVLCKNRYVGRTFIQPNMRLRQLGVAKKFGVLSDNFKGKRIVLVDDSIVRGNTISPIIKLLKESGAKEVHIRVASPPIKYPCFMGINIPTKEELIANKPEFDHLAEYLGANSVVYLSVEGLVSSVQEGIKFKKQKEKKHDIMIQENGNGLECFEKSGHCTACLTGKYPVELEW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Chicken
92
Zebrafish
91
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q06203 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T52 Phosphorylation
K62 Acetylation
K62 Methylation
K62 Ubiquitination
K65 Ubiquitination
K80 Ubiquitination
K81 Acetylation
K81 Ubiquitination
Y94 Phosphorylation
K99 Acetylation
K99 Ubiquitination
R130 Methylation
T166 Phosphorylation
K178 Ubiquitination
K220 Ubiquitination
C339 S-Nitrosylation
C348 S-Nitrosylation
K349 Ubiquitination
T356 Phosphorylation
K371 Acetylation
K372 Acetylation
K372 Ubiquitination
K381 Ubiquitination
K383 Methylation
S399 Phosphorylation
K403 Ubiquitination
K406 Ubiquitination
S408 Phosphorylation
K411 Ubiquitination
K482 Acetylation
K499 Acetylation
K510 Ubiquitination

Research Backgrounds

Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Homotetramer.

Family&Domains:

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.