MYH7 Antibody - #DF12122

(4)   (6)  
Product: MYH7 Antibody
Catalog: DF12122
Description: Rabbit polyclonal antibody to MYH7
Application: WB IHC IF/ICC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 200-220 kDa; 223kD(Calculated).
Uniprot: P12883
RRID: AB_2844927

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MYH7 Antibody detects endogenous levels of total MYH7.
RRID:
AB_2844927
Cite Format: Affinity Biosciences Cat# DF12122, RRID:AB_2844927.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Beta myosin heavy chain; cardiac muscle beta isoform; CMD1S; CMH1; MPD1; MYH7; MYH7_HUMAN; Myhc slow; MyHC-beta; MyHC-slow; MYHCB; Myopathy, distal 1; Myosin heavy chain (AA 1-96); Myosin heavy chain 7; Myosin heavy chain; Myosin heavy chain slow isoform; Myosin heavy chain, cardiac muscle beta isoform; Myosin, heavy chain 7, cardiac muscle, beta; Myosin, heavy polypeptide 7, cardiac muscle, beta; Myosin-7; Rhabdomyosarcoma antigen MU RMS 40.7A; SPMD; SPMM;

Immunogens

Immunogen:

A synthesized peptide derived from human MYH7, corresponding to a region within C-terminal amino acids.

Uniprot:

P12883(MYH7_HUMAN) >>Visit HPA database.

Gene(ID):
MYH7(4625)
Expression:
P12883 MYH7_HUMAN:

Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level).

Sequence:
MGDSEMAVFGAAAPYLRKSEKERLEAQTRPFDLKKDVFVPDDKQEFVKAKIVSREGGKVTAETEYGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKDRYGSWMIYTYSGLFCVTVNPYKWLPVYTPEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQSPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMKFKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVIYATGALAKAVYERMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSANFQKPRNIKGKPEAHFSLIHYAGIVDYNIIGWLQKNKDPLNETVVGLYQKSSLKLLSTLFANYAGADAPIEKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLARMEYKKLLERRDSLLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAEREKEMASMKEEFTRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKMELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGTKGLNEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Pig
92
Bovine
92
Sheep
92
Zebrafish
67
Chicken
55
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.

Subcellular Location:

Cytoplasm>Myofibril. Cytoplasm>Myofibril>Sarcomere.
Note: Thick filaments of the myofibrils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level).

Family&Domains:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils (PubMed:26150528, PubMed:26573747). Four skip residues (Skip1: Thr-1188, Skip2: Glu-1385, Skip3: Glu-1582 and Skip4: Gly-1807) introduce discontinuities in the coiled-coil heptad repeats. The first three skip residues are structurally comparable and induce a unique local relaxation of the coiled-coil superhelical pitch and the fourth skip residue lies within a highly flexible molecular hinge that is necessary for myosin incorporation in the bare zone of sarcomeres (PubMed:26150528).

Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

References

1). Inhibiting RhoA Activation Via GDP-State Stabilization to Relieve Heart Failure. Circulation research, 2026 (PubMed: 41859798) [IF=16.5]

Application: WB    Species: human    Sample:

Figure 5. AH001 suppressed the nuclear translocation of MRTFA in fibroblasts and cardiomyocytes (A) The schema of canonical downstream signaling of RhoA activation and inactivation. (B) The schema of fibroblast activation cellular model construction, followed by treatments of AH001 at doses of 10, 20, and 40 μM. (C and D) The impact of AH001 on the migration of fibroblasts (n = 3) as assessed by the wound healing assay and the Transwell assay. (E) The expression level of RhoA-GTP was detected by the pull-down assay in fibroblasts with the treatment of AH001 at 20 μM. (F and G) The representative immunofluorescence staining against F-actin (F) and MRTFA (G) in fibroblasts, with the quantification of nuclear MRTFA fluorescence signals. Scale bar, 50 μm (F), 20 μm (G). (H and I) The representative immunofluorescence staining against F-actin (H) and MRTFA (I) in fibroblasts followed by the knockdown of RhoGDIα and the treatment of AH001 at 20 μM, with the quantification of nuclear MRTFA fluorescence signals. Scale bar, 20 μm. (J) The schema of the myocardial hypertrophy cellular model construction, followed by treatments of AH001 at doses of 10, 20, and 40 μM. (K) The expression level of RhoA-GTP was detected by the pull-down assay in cardiomyocytes with the treatment of AH001 at 20 μM. (L and M) The representative immunofluorescence staining against F-actin (L) and MRTFA (M) in cardiomyocytes, with the quantification of nuclear MRTFA fluorescence signals. Scale bar, 20 μm. (N) Expression levels of proteins involved in myocardial hypertrophy determined by WB, including MYH7, ANP, BNP, and HSP90. (O and P) The representative immunofluorescence staining against F-actin (O) and MRTFA (P) in cardiomyocytes followed by the knockdown of RhoGDIα and the treatment of AH001 at 20 μM, with the quantification of nuclear MRTFA fluorescence signals. Scale bar, 20 μm. Data are mean ± SEM. Statistical analysis was performed using one-way analysis of variance (ANOVA) among multiple groups, which was followed by an LSD post hoc test between two groups.
2). Comparative Analysis of Muscle Fibers in Selected Muscles of Working and Companion Dog Breeds. Animals : an open access journal from MDPI, 2024 (PubMed: 39765480) [IF=3.0]

Application: IHC    Species: dog    Sample:

Figure 2. Immunohistochemical staining of muscle fibers in the triceps brachii of companion and working dog breeds, illustrating the distribution of different myosin heavy chain (MYH) isoforms. Staining was performed using DAB as a chromogen. The rows represent different myosin heavy chain isoforms, with MYH2, MYH4, and MYH7 detected in companion and working breeds. Arrows indicate immunoreactive muscle fibers displaying the respective MYH isoform. Scale bars are included in each image for reference (100 µm and 200 µm).
3). Development of CRISRP/Cas9-based TP53-knockout pig muscle stem cells for use in the cultured meat industry. 3 Biotech, 2025 (PubMed: 40115325) [IF=2.6]
4). Hoxa11 and Hoxa13 facilitate slow‐twitch muscle formation in C2C12 cells and indirectly affect the lipid deposition of 3T3‐L1 cells. ANIMAL SCIENCE JOURNAL, 2021 (PubMed: 33738916) [IF=1.7]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.