MYH7 Antibody - #DF12122

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Product: MYH7 Antibody
Catalog: DF12122
Description: Rabbit polyclonal antibody to MYH7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 200-220 kDa; 223kD(Calculated).
Uniprot: P12883
RRID: AB_2844927

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MYH7 Antibody detects endogenous levels of total MYH7.
RRID:
AB_2844927
Cite Format: Affinity Biosciences Cat# DF12122, RRID:AB_2844927.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Beta myosin heavy chain; cardiac muscle beta isoform; CMD1S; CMH1; MPD1; MYH7; MYH7_HUMAN; Myhc slow; MyHC-beta; MyHC-slow; MYHCB; Myopathy, distal 1; Myosin heavy chain (AA 1-96); Myosin heavy chain 7; Myosin heavy chain; Myosin heavy chain slow isoform; Myosin heavy chain, cardiac muscle beta isoform; Myosin, heavy chain 7, cardiac muscle, beta; Myosin, heavy polypeptide 7, cardiac muscle, beta; Myosin-7; Rhabdomyosarcoma antigen MU RMS 40.7A; SPMD; SPMM;

Immunogens

Immunogen:
Uniprot:

P12883(MYH7_HUMAN) >>Visit HPA database.

Gene(ID):
MYH7(4625)
Expression:
P12883 MYH7_HUMAN:

Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level).

Sequence:
MGDSEMAVFGAAAPYLRKSEKERLEAQTRPFDLKKDVFVPDDKQEFVKAKIVSREGGKVTAETEYGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKDRYGSWMIYTYSGLFCVTVNPYKWLPVYTPEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQSPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMKFKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVIYATGALAKAVYERMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSANFQKPRNIKGKPEAHFSLIHYAGIVDYNIIGWLQKNKDPLNETVVGLYQKSSLKLLSTLFANYAGADAPIEKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLARMEYKKLLERRDSLLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAEREKEMASMKEEFTRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKMELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGTKGLNEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Pig
92
Bovine
92
Sheep
92
Zebrafish
67
Chicken
55
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12883 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
Y15 Phosphorylation
S19 Phosphorylation
K34 Acetylation
K35 Acetylation
S53 Phosphorylation
T60 Phosphorylation
T63 Phosphorylation
Y65 Phosphorylation
T70 Phosphorylation
Y103 Phosphorylation
K129 Acetylation
Y162 Phosphorylation
Y164 Phosphorylation
T167 Phosphorylation
S173 Phosphorylation
S210 Phosphorylation
T215 Phosphorylation
K246 Ubiquitination
T255 Phosphorylation
S260 Phosphorylation
Y266 Phosphorylation
K270 Ubiquitination
K363 Acetylation
T378 Phosphorylation
Y386 Phosphorylation
Y410 Phosphorylation
T412 Phosphorylation
K413 Acetylation
Y422 Phosphorylation
T424 Phosphorylation
T441 Phosphorylation
T446 Phosphorylation
T449 Phosphorylation
K502 Acetylation
K549 Acetylation
S560 Phosphorylation
Y582 Phosphorylation
Y588 Phosphorylation
Y609 Phosphorylation
Y624 Phosphorylation
T660 Phosphorylation
T678 Phosphorylation
S680 Phosphorylation
Y715 Phosphorylation
S738 Phosphorylation
Y756 Phosphorylation
K757 Acetylation
S782 Phosphorylation
T786 Phosphorylation
S810 Phosphorylation
S842 Phosphorylation
S851 Phosphorylation
K853 Acetylation
T857 Phosphorylation
K865 Acetylation
S866 Phosphorylation
S879 Phosphorylation
T937 Phosphorylation
K939 Acetylation
K942 Acetylation
S948 Phosphorylation
K951 Acetylation
T960 Phosphorylation
T979 Phosphorylation
T993 Phosphorylation
T1019 Phosphorylation
S1037 Phosphorylation
K1042 Acetylation
K1043 Acetylation
K1052 Acetylation
S1065 Phosphorylation
S1102 O-Glycosylation
S1102 Phosphorylation
K1106 Acetylation
K1107 Acetylation
T1127 Phosphorylation
S1137 Phosphorylation
S1140 Phosphorylation
S1158 Phosphorylation
K1165 Acetylation
T1182 Phosphorylation
T1188 Phosphorylation
S1199 Phosphorylation
S1222 Phosphorylation
T1232 Phosphorylation
S1233 Phosphorylation
S1261 Phosphorylation
T1266 Phosphorylation
S1269 Phosphorylation
T1274 Phosphorylation
S1275 Phosphorylation
T1282 Phosphorylation
S1288 Phosphorylation
S1299 O-Glycosylation
S1299 Phosphorylation
T1302 Phosphorylation
K1305 Acetylation
T1307 Phosphorylation
Y1308 Phosphorylation
T1309 Phosphorylation
K1316 Acetylation
K1324 Methylation
S1335 Phosphorylation
Y1347 Phosphorylation
T1351 Phosphorylation
S1362 Phosphorylation
S1366 Phosphorylation
T1373 Phosphorylation
Y1375 Phosphorylation
T1377 Phosphorylation
T1383 Phosphorylation
K1390 Acetylation
K1391 Acetylation
K1392 Acetylation
S1412 Phosphorylation
S1413 Phosphorylation
K1444 Acetylation
K1445 Acetylation
Y1460 Phosphorylation
S1463 Phosphorylation
S1465 Phosphorylation
S1469 Phosphorylation
S1470 Phosphorylation
S1476 Phosphorylation
S1478 Phosphorylation
T1479 Phosphorylation
Y1488 Phosphorylation
S1491 Phosphorylation
T1497 Phosphorylation
K1499 Acetylation
S1510 Phosphorylation
T1513 Phosphorylation
S1518 Phosphorylation
S1519 Phosphorylation
T1522 Phosphorylation
S1542 Phosphorylation
S1550 Phosphorylation
K1587 Acetylation
S1596 O-Glycosylation
S1596 Phosphorylation
T1599 Phosphorylation
S1600 Phosphorylation
T1605 Phosphorylation
S1607 Phosphorylation
S1630 Phosphorylation
S1645 Phosphorylation
S1648 Phosphorylation
S1710 O-Glycosylation
S1710 Phosphorylation
S1718 Phosphorylation
T1721 Phosphorylation
S1722 Phosphorylation
K1727 Acetylation
S1735 Phosphorylation
T1739 Phosphorylation
T1760 Phosphorylation
K1770 Acetylation
K1784 Acetylation
K1806 Acetylation
K1809 Acetylation
K1810 Acetylation
K1831 Acetylation
K1848 Ubiquitination
T1851 Phosphorylation
Y1852 Phosphorylation
T1854 Phosphorylation
K1859 Acetylation
T1891 Phosphorylation
S1894 Phosphorylation
S1915 Phosphorylation

Research Backgrounds

Function:

Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.

Subcellular Location:

Cytoplasm>Myofibril. Cytoplasm>Myofibril>Sarcomere.
Note: Thick filaments of the myofibrils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level).

Subunit Structure:

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with ECPAS. Interacts (via C-terminus) with LRRC39.

Family&Domains:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils (PubMed:26150528, PubMed:26573747). Four skip residues (Skip1: Thr-1188, Skip2: Glu-1385, Skip3: Glu-1582 and Skip4: Gly-1807) introduce discontinuities in the coiled-coil heptad repeats. The first three skip residues are structurally comparable and induce a unique local relaxation of the coiled-coil superhelical pitch and the fourth skip residue lies within a highly flexible molecular hinge that is necessary for myosin incorporation in the bare zone of sarcomeres (PubMed:26150528).

Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

References

1). Hoxa11 and Hoxa13 facilitate slow‐twitch muscle formation in C2C12 cells and indirectly affect the lipid deposition of 3T3‐L1 cells. ANIMAL SCIENCE JOURNAL (PubMed: 33738916) [IF=2.0]

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