ERp72 Antibody - #DF12114

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Product: ERp72 Antibody
Catalog: DF12114
Description: Rabbit polyclonal antibody to ERp72
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Rabbit, Chicken
Mol.Wt.: 72 kDa; 73kD(Calculated).
Uniprot: P13667
RRID: AB_2844919

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(92%), Rabbit(92%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
ERp72 Antibody detects endogenous levels of total ERp72.
RRID:
AB_2844919
Cite Format: Affinity Biosciences Cat# DF12114, RRID:AB_2844919.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Calcium binding protein intestinal related; Endoplasmic reticulum resident protein 70; Endoplasmic reticulum resident protein 72; EPR70; EPR72; ER protein 70; ER protein 72; ERP 70; ERp 72; ERp-72; ERp70; ERp72; PDIA 4; Pdia4; PDIA4_HUMAN; PDIR; Protein disulfide isomerase A4; Protein disulfide isomerase associated 4; Protein disulfide isomerase family A member 4; protein disulfide isomerase related protein (calcium-binding protein, intestinal-related); Protein disulfide isomerase related protein; Protein disulfide-isomerase A4; Protein ERp 72; Protein ERp72;

Immunogens

Immunogen:
Uniprot:

P13667(PDIA4_HUMAN) >>Visit HPA database.

Gene(ID):
PDIA4(9601)
Sequence:
MRPRKAFLLLLLLGLVQLLAVAGAEGPDEDSSNRENAIEDEEEEEEEDDDEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEIVAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFRKGRPYDYNGPREKYGIVDYMIEQSGPPSKEILTLKQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
92
Horse
92
Chicken
83
Pig
75
Bovine
75
Sheep
75
Dog
75
Xenopus
67
Zebrafish
50
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13667 As Substrate

Site PTM Type Enzyme
Y101 Phosphorylation
K103 Ubiquitination
K119 Ubiquitination
S124 Phosphorylation
S130 Phosphorylation
Y137 Phosphorylation
K141 Ubiquitination
K163 Ubiquitination
S168 Phosphorylation
T173 Phosphorylation
K218 Ubiquitination
S227 Phosphorylation
K234 Ubiquitination
T238 Phosphorylation
T241 Phosphorylation
K245 Ubiquitination
Y252 Phosphorylation
T254 Phosphorylation
K256 Acetylation
K256 Ubiquitination
Y266 Phosphorylation
R270 Methylation
Y273 Phosphorylation
Y278 Phosphorylation
S287 Phosphorylation
K294 Ubiquitination
Y320 Phosphorylation
Y323 Phosphorylation
S351 Phosphorylation
K366 Acetylation
Y367 Phosphorylation
S379 Phosphorylation
T380 Phosphorylation
S383 Phosphorylation
K391 Ubiquitination
Y392 Phosphorylation
S403 Phosphorylation
Y409 Phosphorylation
T410 Phosphorylation
Y448 Phosphorylation
T449 Phosphorylation
Y458 Phosphorylation
S468 Phosphorylation
S470 Phosphorylation
K484 Acetylation
K484 Ubiquitination
K485 Acetylation
S495 Phosphorylation
T503 O-Glycosylation
K506 Acetylation
K509 Acetylation
S516 Phosphorylation
K533 Acetylation
Y551 Phosphorylation
Y565 Phosphorylation
S567 Phosphorylation
K576 Ubiquitination
T586 Phosphorylation
S592 Phosphorylation
R594 Methylation
K596 Acetylation
Y604 Phosphorylation
S608 Phosphorylation

Research Backgrounds

Subcellular Location:

Endoplasmic reticulum lumen. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Component of a complex containing at least CRELD2, MANF, MATN3 and PDIA4 (By similarity).

Family&Domains:

Belongs to the protein disulfide isomerase family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

Restrictive clause

 

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For Research Use Only.
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