CCT3 Antibody - #DF12113

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Product: CCT3 Antibody
Catalog: DF12113
Description: Rabbit polyclonal antibody to CCT3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 60 kDa; 61kD(Calculated).
Uniprot: P49368
RRID: AB_2844918

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CCT3 Antibody detects endogenous levels of total CCT3.
RRID:
AB_2844918
Cite Format: Affinity Biosciences Cat# DF12113, RRID:AB_2844918.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CCT 3; CCT gamma; CCT-gamma; CCT3; CCTG; Chaperonin containing TCP1 subunit 3 (gamma); Chaperonin containing TCP1 subunit 3; hTRiC5; PIG48; T complex protein 1 gamma subunit; T complex protein 1 subunit gamma; T-complex protein 1 subunit gamma; TCP 1 gamma; TCP-1-gamma; TCP1 (t complex 1) ring complex polypeptide 5; TCPG_HUMAN; TRIC5;

Immunogens

Immunogen:
Uniprot:

P49368(TCPG_HUMAN) >>Visit HPA database.

Gene(ID):
CCT3(7203)
Sequence:
MMGHRPVLVLSQNTKRESGRKVQSGNINAAKTIADIIRTCLGPKSMMKMLLDPMGGIVMTNDGNAILREIQVQHPAAKSMIEISRTQDEEVGDGTTSVIILAGEMLSVAEHFLEQQMHPTVVISAYRKALDDMISTLKKISIPVDISDSDMMLNIINSSITTKAISRWSSLACNIALDAVKMVQFEENGRKEIDIKKYARVEKIPGGIIEDSCVLRGVMINKDVTHPRMRRYIKNPRIVLLDSSLEYKKGESQTDIEITREEDFTRILQMEEEYIQQLCEDIIQLKPDVVITEKGISDLAQHYLMRANITAIRRVRKTDNNRIARACGARIVSRPEELREDDVGTGAGLLEIKKIGDEYFTFITDCKDPKACTILLRGASKEILSEVERNLQDAMQVCRNVLLDPQLVPGGGASEMAVAHALTEKSKAMTGVEQWPYRAVAQALEVIPRTLIQNCGASTIRLLTSLRAKHTQENCETWGVNGETGTLVDMKELGIWEPLAVKLQTYKTAVETAVLLLRIDDIVSGHKKKGDDQSRQGGAPDAGQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Pig
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49368 As Substrate

Site PTM Type Enzyme
M1 Acetylation
R5 Methylation
S11 Phosphorylation
T14 Phosphorylation
K15 Ubiquitination
K21 Acetylation
K21 Ubiquitination
S24 Phosphorylation
K31 Acetylation
K31 Ubiquitination
K44 Acetylation
K44 Ubiquitination
K78 Ubiquitination
T86 Phosphorylation
K128 Ubiquitination
S135 Phosphorylation
T136 Phosphorylation
K138 Ubiquitination
K139 Ubiquitination
S149 Phosphorylation
S170 Phosphorylation
C173 S-Nitrosylation
K181 Ubiquitination
Y198 Phosphorylation
K203 Ubiquitination
C213 S-Nitrosylation
R216 Methylation
K222 Acetylation
K222 Ubiquitination
T225 Phosphorylation
Y232 Phosphorylation
K234 Ubiquitination
S243 Phosphorylation
S244 Phosphorylation
Y247 Phosphorylation
K248 Acetylation
K248 Ubiquitination
K249 Ubiquitination
S252 Phosphorylation
T254 Phosphorylation
T259 Phosphorylation
K286 Ubiquitination
S297 Phosphorylation
Y303 Phosphorylation
T310 Phosphorylation
R313 Methylation
K317 Acetylation
K353 Ubiquitination
K354 Ubiquitination
Y359 Phosphorylation
K367 Ubiquitination
K370 Acetylation
K370 Ubiquitination
R377 Methylation
S380 Phosphorylation
K381 Acetylation
K381 Sumoylation
K381 Ubiquitination
S385 Phosphorylation
K425 Ubiquitination
K427 Ubiquitination
T430 Phosphorylation
C455 S-Nitrosylation
S458 Phosphorylation
T459 Phosphorylation
K469 Ubiquitination
T477 Phosphorylation
K491 Ubiquitination
K502 Ubiquitination
K507 Ubiquitination
T508 Phosphorylation
T512 Phosphorylation
S524 Phosphorylation
K527 Acetylation
K527 Ubiquitination
K528 Ubiquitination
K529 Ubiquitination
S534 Phosphorylation

Research Backgrounds

Function:

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the chaperonin-containing T-complex (TRiC), a heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Interacts with DNAAF4 (By similarity).

Family&Domains:

Belongs to the TCP-1 chaperonin family.

References

1). Integrated analyzes identify CCT3 as a modulator to shape immunosuppressive tumor microenvironment in lung adenocarcinoma. BMC CANCER, 2023 (PubMed: 36918801) [IF=3.8]

Application: WB    Species: Human    Sample: LUAD A549 cells

Fig. 11 CCT3 knockdown suppressed colony formation and cell proliferation but induced apoptosis of LUAD A549 cells. (a, b) Western blotting (a) and band intensity (b) analyzes of small interfering RNA mediated CCT3 knockdown in A549 cells, GAPDH was used as the loading control. (c, d) Representative colony formation (c) and comparisons of colony counts (d) between si-CCT3 and si-Control cells. (e) The proliferation of si-CCT3 and si-Control A549 cells were determined by WST-8/Cell Counting Kit-8 at 0, 24 and 48 h after cell culturing. (f, g) Representative flow cytometry (f) and comparisons of percentages of total Annexin-V+ cells (g) between si-CCT3 and si-Control A549 cells. Data are representative of three independent experiments. **P 

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