ATPB Antibody - #DF12111

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Product: ATPB Antibody
Catalog: DF12111
Description: Rabbit polyclonal antibody to ATPB
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Chicken
Mol.Wt.: 45-57 kDa; 57kD(Calculated).
Uniprot: P06576
RRID: AB_2844916

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
ATPB Antibody detects endogenous levels of total ATPB.
RRID:
AB_2844916
Cite Format: Affinity Biosciences Cat# DF12111, RRID:AB_2844916.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP 5B; ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide; ATP synthase subunit beta mitochondrial; ATP synthase subunit beta, mitochondrial; atp5b; ATPB; ATPB_HUMAN; ATPMB; ATPSB; Epididymis secretory protein Li 271; HEL-S-271; Mitochondrial ATP synthase beta subunit; Mitochondrial ATP Synthase Subunit Beta; Mitochondrial ATP synthetase beta subunit;

Immunogens

Immunogen:

A synthesized peptide derived from human ATPB, corresponding to a region within the internal amino acids.

Uniprot:

P06576(ATPB_HUMAN) >>Visit HPA database.

Gene(ID):
ATP5B(506)
Sequence:
MLGFVGRVAAAPASGALRRLTPSASLPPAQLLLRAAPTAVHPVRDYAAQTSPSPKAGAATGRIVAVIGAVVDVQFDEGLPPILNALEVQGRETRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVLDSGAPIKIPVGPETLGRIMNVIGEPIDERGPIKTKQFAPIHAEAPEFMEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Chicken
100
Rabbit
100
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Subcellular Location:

Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the ATPase alpha/beta chains family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Ubiquitin-specific peptidase 10 promotes renal interstitial fibrosis progression through deubiquitinating and stabilizing P53 protein. Biochimica et biophysica acta. Molecular basis of disease, 2025 (PubMed: 39788218) [IF=6.2]
2). Umbelliferone attenuates cisplatin-induced acute kidney injury by inhibiting oxidative stress and inflammation via NRF2. Physiological reports, 2023 (PubMed: 38030388) [IF=2.2]

Application: WB    Species: Mouse    Sample:

FIGURE 7 UMB (40 mg/kg) inhibited cisplatin‐induced oxidative stress by acting on NRF2 in mice. (a, b, c) Quantitative PCR was performed to analyze mRNA levels of renal HO‐1, SOD2, and ATP8. (d) Quantitative PCR analysis of mtDNA copy number in the kidneys of mice. 18S rDNA was used as an internal control. (e) Quantitative PCR was performed to analyze mRNA levels of renal PGC‐1α. (f) Western blot analysis of SOD2 and ATPB levels in the kidneys of each group of mice. (g & h) SOD2 and ATPB densitometry analysis. Data are shown as means ± standard deviation (n = 6 mice/group).

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