Cyclin L1 Antibody - #AF0236

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Product: Cyclin L1 Antibody
Catalog: AF0236
Description: Rabbit polyclonal antibody to Cyclin L1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 60kDa; 60kD(Calculated).
Uniprot: Q9UK58
RRID: AB_2833411

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Cyclin L1 Antibody detects endogenous levels of total Cyclin L1.
RRID:
AB_2833411
Cite Format: Affinity Biosciences Cat# AF0236, RRID:AB_2833411.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Ania 6a; CCNL1; CCNL1_HUMAN; Cyclin L; Cyclin L gamma; Cyclin L1; Cyclin-L; Cyclin-L1;

Immunogens

Immunogen:
Uniprot:

Q9UK58(CCNL1_HUMAN) >>Visit HPA database.

Gene(ID):
CCNL1(57018)
Expression:
Q9UK58 CCNL1_HUMAN:

Widely expressed. Overexpression in primary tumors of head and neck squamous cell carcinomas (HNSCC).

Description:
CCNL1 cyclin L1, a member of the cyclin family. Associates with the PITSLRE kinase and is involved in pre-mRNA processing.
Sequence:
MASGPHSTATAAAAASSAAPSAGGSSSGTTTTTTTTTGGILIGDRLYSEVSLTIDHSLIPEERLSPTPSMQDGLDLPSETDLRILGCELIQAAGILLRLPQVAMATGQVLFHRFFYSKSFVKHSFEIVAMACINLASKIEEAPRRIRDVINVFHHLRQLRGKRTPSPLILDQNYINTKNQVIKAERRVLKELGFCVHVKHPHKIIVMYLQVLECERNQTLVQTAWNYMNDSLRTNVFVRFQPETIACACIYLAARALQIPLPTRPHWFLLFGTTEEEIQEICIETLRLYTRKKPNYELLEKEVEKRKVALQEAKLKAKGLNPDGTPALSTLGGFSPASKPSSPREVKAEEKSPISINVKTVKKEPEDRQQASKSPYNGVRKDSKRSRNSRSASRSRSRTRSRSRSHTPRRHYNNRRSRSGTYSSRSRSRSRSHSESPRRHHNHGSPHLKAKHTRDDLKSSNRHGHKRKKSRSRSQSKSRDHSDAAKKHRHERGHHRDRRERSRSFERSHKSKHHGGSRSGHGRHRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
75
Zebrafish
46
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UK58 As Substrate

Site PTM Type Enzyme
T30 Phosphorylation
T32 Phosphorylation
T33 Phosphorylation
T37 Phosphorylation
S65 Phosphorylation
T67 Phosphorylation
S166 Phosphorylation
K178 Ubiquitination
Y289 Phosphorylation
T325 Phosphorylation
S329 Phosphorylation
T330 Phosphorylation
S335 Phosphorylation
S338 Phosphorylation
S341 Phosphorylation
S342 Phosphorylation
S352 Phosphorylation
S355 Phosphorylation
S372 Phosphorylation
S374 Phosphorylation
Y376 Phosphorylation
S401 Phosphorylation
S403 Phosphorylation
S405 Phosphorylation
S417 Phosphorylation
S419 Phosphorylation
T421 Phosphorylation
Y422 Phosphorylation
S424 Phosphorylation
S445 Phosphorylation
K449 Acetylation
K451 Acetylation
S470 Phosphorylation
R471 Methylation
R473 Methylation
K486 Ubiquitination
K487 Ubiquitination
S502 Phosphorylation
S504 Phosphorylation
K512 Methylation

Research Backgrounds

Function:

Involved in pre-mRNA splicing. Functions in association with cyclin-dependent kinases (CDKs). Inhibited by the CDK-specific inhibitor CDKN1A/p21. May play a role in the regulation of RNA polymerase II (pol II). May be a candidate proto-oncogene in head and neck squamous cell carcinomas (HNSCC).

Subcellular Location:

Nucleus speckle. Nucleus>Nucleoplasm.
Note: Found in nuclear intrachromatin granules clusters (IGC), also called nuclear speckles, which are storage compartments for nuclear proteins involved in mRNA processing.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Overexpression in primary tumors of head and neck squamous cell carcinomas (HNSCC).

Subunit Structure:

Interacts with POLR2A via its hyperphosphorylated C-terminal domain (CTD) (By similarity). Interacts with CDK11A, CDK12 and CDK13. Isoforms 1 and 2, but not isoform 3, interact with CDK11B. May form a ternary complex with CDK11B and casein kinase II (CKII). Interacts with pre-mRNA-splicing factors, including at least SRSF1, SRSF2 AND SRSF7/SLU7.

Family&Domains:

Contains a RS region (arginine-serine dipeptide repeat) within the C-terminal domain which is the hallmark of the SR family of splicing factors. This region probably plays a role in protein-protein interactions.

Belongs to the cyclin family. Cyclin L subfamily.

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