RDH10 Antibody - #DF12105

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Product: RDH10 Antibody
Catalog: DF12105
Description: Rabbit polyclonal antibody to RDH10
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 39 kDa; 38kD(Calculated).
Uniprot: Q8IZV5
RRID: AB_2844910

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RDH10 Antibody detects endogenous levels of total RDH10.
RRID:
AB_2844910
Cite Format: Affinity Biosciences Cat# DF12105, RRID:AB_2844910.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

RDH 10; RDH10; RDH10_HUMAN; Retinol dehydrogenase 10 (all trans); Retinol dehydrogenase 10; SDR16C4; Short chain dehydrogenase/reductase family 16C member 4;

Immunogens

Immunogen:
Uniprot:

Q8IZV5(RDH10_HUMAN) >>Visit HPA database.

Gene(ID):
RDH10(157506)
Expression:
Q8IZV5 RDH10_HUMAN:

Detected in retina, kidney, liver, small intestine, placenta, lung, heart and skeletal muscle.

Sequence:
MNIVVEFFVVTFKVLWAFVLAAARWLVRPKEKSVAGQVCLITGAGSGLGRLFALEFARRRALLVLWDINTQSNEETAGMVRHIYRDLEAADAAALQAGNGEEEILPHCNLQVFTYTCDVGKRENVYLTAERVRKEVGEVSVLVNNAGVVSGHHLLECPDELIERTMMVNCHAHFWTTKAFLPTMLEINHGHIVTVASSLGLFSTAGVEDYCASKFGVVGFHESLSHELKAAEKDGIKTTLVCPYLVDTGMFRGCRIRKEIEPFLPPLKPDYCVKQAMKAILTDQPMICTPRLMYIVTFMKSILPFEAVVCMYRFLGADKCMYPFIAQRKQATNNNEAKNGI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
88
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IZV5 As Substrate

Site PTM Type Enzyme
K121 Ubiquitination
K134 Ubiquitination
K229 Ubiquitination
T248 Phosphorylation
K268 Ubiquitination
K274 Ubiquitination
Y294 Phosphorylation
T297 Phosphorylation
K319 Ubiquitination
Y322 Phosphorylation
K338 Ubiquitination

Research Backgrounds

Function:

Retinol dehydrogenase with a clear preference for NADP. Converts all-trans-retinol to all-trans-retinal. Has no detectable activity towards 11-cis-retinol, 9-cis-retinol and 13-cis-retinol.

Subcellular Location:

Microsome membrane>Single-pass membrane protein. Endoplasmic reticulum membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in retina, kidney, liver, small intestine, placenta, lung, heart and skeletal muscle.

Family&Domains:

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Novel Potential Markers of Myofibroblast Differentiation Revealed by Single-Cell RNA Sequencing Analysis of Mesenchymal Stromal Cells in Profibrotic and Adipogenic Conditions. Biomedicines (PubMed: 36979822) [IF=4.7]

Application: IF/ICC    Species: Human    Sample: adipose tissue

Figure 4. Immunocytochemical evaluation of CHD3 (green), RDH10 (green), NTM (green), and αSMA (red) in the control (Control) MSCs and after incubation in profibrotic (F) or adipogenic conditions (Ad). Fluorescent microscopy, blue staining—nuclei stained with DAPI, scale bar is 50 µm (A). The percentage of CHD3-positive cells, mean ± SE, p value is indicated (B). The percentage of cells with neurotrimin clusters, mean ± SE, p value is indicated (C).

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