LDHB Antibody - #DF12101

(3)  
Product: LDHB Antibody
Catalog: DF12101
Description: Rabbit polyclonal antibody to LDHB
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse
Mol.Wt.: 35 kDa; 37kD(Calculated).
Uniprot: P07195
RRID: AB_2844906

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(100%)
Clonality:
Polyclonal
Specificity:
LDHB Antibody detects endogenous levels of total LDHB.
RRID:
AB_2844906
Cite Format: Affinity Biosciences Cat# DF12101, RRID:AB_2844906.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Epididymis secretory protein Li 281; HEL S 281; L lactate dehydrogenase B chain; L-lactate dehydrogenase B chain; Lactate dehydrogenase H chain; LDH B; LDH H; LDH heart subunit; LDH-B; LDH-H; LDHB; LDHB_HUMAN; LDHBD; LDHH; Renal carcinoma antigen NY REN 46; Renal carcinoma antigen NY-REN-46; TRG-5; TRG5;

Immunogens

Immunogen:
Uniprot:

P07195(LDHB_HUMAN) >>Visit HPA database.

Gene(ID):
LDHB(3945)
Sequence:
MATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKSADTLWDIQKDLKDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
75
Chicken
71
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07195 As Substrate

Site PTM Type Enzyme
K5 Ubiquitination
K7 Acetylation
K7 Ubiquitination
T18 Phosphorylation
S39 Phosphorylation
K43 Ubiquitination
S44 Phosphorylation
K58 Acetylation
K58 Ubiquitination
K60 Ubiquitination
T75 Phosphorylation
K77 Ubiquitination
K82 Acetylation
K82 Ubiquitination
Y84 Phosphorylation
S85 Phosphorylation
S90 Phosphorylation
K91 Ubiquitination
T96 Phosphorylation
S106 Phosphorylation
K119 Acetylation
K119 Methylation
K119 Ubiquitination
K156 Acetylation
K156 Ubiquitination
R158 Methylation
S162 Phosphorylation
S168 Phosphorylation
R170 Methylation
R172 Methylation
Y173 Phosphorylation
K229 Acetylation
K233 Ubiquitination
S238 Phosphorylation
Y240 Phosphorylation
K244 Acetylation
K244 Ubiquitination
K246 Ubiquitination
Y248 Phosphorylation
T249 Phosphorylation
S269 Phosphorylation
S291 Phosphorylation
C294 S-Nitrosylation
T302 Phosphorylation
S303 Phosphorylation
K308 Acetylation
K308 Ubiquitination
K310 Acetylation
K310 Sumoylation
K310 Ubiquitination
K318 Acetylation
K318 Ubiquitination
K319 Acetylation
K319 Ubiquitination
S320 Phosphorylation
T323 Phosphorylation
K329 Acetylation
K329 Ubiquitination
K332 Acetylation
K332 Sumoylation
K332 Ubiquitination

Research Backgrounds

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the LDH/MDH superfamily. LDH family.

Research Fields

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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