HMGCS1 Antibody - #DF12100

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Product: HMGCS1 Antibody
Catalog: DF12100
Description: Rabbit polyclonal antibody to HMGCS1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 57 kDa; 57kD(Calculated).
Uniprot: Q01581
RRID: AB_2844905

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(86%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
HMGCS1 Antibody detects endogenous levels of total HMGCS1.
RRID:
AB_2844905
Cite Format: Affinity Biosciences Cat# DF12100, RRID:AB_2844905.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble); 3-hydroxy-3-methylglutaryl coenzyme A synthase; cytoplasmic; EC 2.3.3.10; HMCS1_HUMAN; HMG CoA synthase; HMG-CoA synthase; HMGCS; HMGCS1; Hydroxymethylglutaryl CoA synthase, cytoplasmic; Hydroxymethylglutaryl-CoA synthase; MGC90332;

Immunogens

Immunogen:
Uniprot:

Q01581(HMCS1_HUMAN) >>Visit HPA database.

Gene(ID):
HMGCS1(3157)
Sequence:
MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
86
Pig
0
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01581 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
K46 Acetylation
K46 Ubiquitination
S67 Phosphorylation
C86 S-Nitrosylation
T96 Phosphorylation
K100 Ubiquitination
S101 Phosphorylation
K206 Acetylation
K206 Ubiquitination
Y213 Phosphorylation
S221 Phosphorylation
S227 Phosphorylation
K239 Ubiquitination
K246 Acetylation
K246 Ubiquitination
K273 Acetylation
K273 Ubiquitination
K291 Ubiquitination
S293 Phosphorylation
S296 Phosphorylation
K305 Sumoylation
K305 Ubiquitination
Y310 Phosphorylation
R313 Methylation
K317 Acetylation
K317 Ubiquitination
K321 Acetylation
K321 Ubiquitination
K330 Ubiquitination
S361 Phosphorylation
K368 Ubiquitination
K400 Ubiquitination
K409 Acetylation
K409 Ubiquitination
S414 Phosphorylation
K428 Ubiquitination
T471 Phosphorylation
T476 Phosphorylation
S486 Phosphorylation
T490 Phosphorylation
S495 Phosphorylation P06493 (CDK1)
K498 Ubiquitination
K499 Ubiquitination
S516 Phosphorylation

Research Backgrounds

Function:

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the thiolase-like superfamily. HMG-CoA synthase family.

Research Fields

· Metabolism > Lipid metabolism > Synthesis and degradation of ketone bodies.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Carbohydrate metabolism > Butanoate metabolism.

· Metabolism > Metabolism of terpenoids and polyketides > Terpenoid backbone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

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