SQRDL Antibody | Affinity Biosciences

WB
IHC
IF/ICC
Cites

1/8

Western blot analysis of extracts from HepG2 cells, using SQRDL antibody.

Western blot analysis of extracts from hela, using SQRDL antibody.

DF12087 at 1/100 staining Rat muscle tissue by IHC-P.

DF12087 at 1/100 staining Rat skin tissue by IHC-P.

DF12087 at 1/100 staining Mouse muscle tissue by IHC-P.

DF12087 staining Hela cells by IF/ICC.

Fig.

Product:	SQRDL Antibody
Catalog:	DF12087
Description:	Rabbit polyclonal antibody to SQRDL
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	50kd; 50kD(Calculated).
Uniprot:	Q9Y6N5
RRID:	AB_2844892

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(86%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

SQRDL Antibody detects endogenous levels of total SQRDL.

RRID:

AB_2844892
Cite Format: Affinity Biosciences Cat# DF12087, RRID:AB_2844892.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

0610039J17Rik; 4930557M22Rik; CGI 44; CGI44; mitochondrial; PRO1975; SQRD_HUMAN; Sqrdl; Sulfide dehydrogenase like; Sulfide quinone reductase like; Sulfide quinone reductase-like (yeast); Sulfide:quinone oxidoreductase; Sulfide:quinone oxidoreductase, mitochondrial;

Immunogens

Immunogen:

Uniprot:

Q9Y6N5(SQOR_HUMAN) >>Visit HPA database.

Gene(ID):

SQOR(58472)

Sequence:

Q9Y6N5 SQOR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVPLVAVVSGPRAQLFACLLRLGTQQVGPLQLHTGASHAARNHYEVLVLGGGSGGITMAARMKRKVGAENVAIVEPSERHFYQPIWTLVGAGAKQLSSSGRPTASVIPSGVEWIKARVTELNPDKNCIHTDDDEKISYRYLIIALGIQLDYEKIKGLPEGFAHPKIGSNYSVKTVEKTWKALQDFKEGNAIFTFPNTPVKCAGAPQKIMYLSEAYFRKTGKRSKANIIFNTSLGAIFGVKKYADALQEIIQERNLTVNYKKNLIEVRADKQEAVFENLDKPGETQVISYEMLHVTPPMSPPDVLKTSPVADAAGWVDVDKETLQHRRYPNVFGIGDCTNLPTSKTAAAVAAQSGILDRTISVIMKNQTPTKKYDGYTSCPLVTGYNRVILAEFDYKAEPLETFPFDQSKERLSMYLMKADLMPFLYWNMMLRGYWGGPAFLRKLFHLGMS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Rabbit

100

Sheep

Zebrafish

Xenopus

Bovine

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6N5 As Substrate

Q9Y6N5

Site	PTM Type	Source
K115	Acetylation	Uniprot
K125	Ubiquitination	Uniprot
K135	Acetylation	Uniprot
S137	Phosphorylation	Uniprot
Y151	Phosphorylation	Uniprot
K155	Ubiquitination	Uniprot
K165	Ubiquitination	Uniprot
K173	Acetylation	Uniprot
K173	Ubiquitination	Uniprot
K177	Ubiquitination	Uniprot
K180	Acetylation	Uniprot
K186	Acetylation	Uniprot
K186	Ubiquitination	Uniprot
T197	Phosphorylation	Uniprot
Y259	Phosphorylation	Uniprot
K260	Acetylation	Uniprot
K261	Ubiquitination	Uniprot
K320	Acetylation	Uniprot
T338	Phosphorylation	Uniprot
T342	Phosphorylation	Uniprot
S343	Phosphorylation	Uniprot
S361	Phosphorylation	Uniprot
Y395	Phosphorylation	Uniprot
S408	Phosphorylation	Uniprot
K409	Ubiquitination	Uniprot
S413	Phosphorylation	Uniprot
Y415	Phosphorylation	Uniprot
K443	Acetylation	Uniprot
S450	Phosphorylation	Uniprot

Research Backgrounds

Q9Y6N5_SQOR_HUMAN

Function:

Catalyzes the oxidation of hydrogen sulfide with the help of a quinone, such as ubiquinone, giving rise to thiosulfate and ultimately to sulfane (molecular sulfur) atoms. Requires an additional electron acceptor; can use sulfite, sulfide or cyanide (in vitro).

Subcellular Location:

Mitochondrion.

Family&Domains:

Belongs to the SQRD family.

Research Fields

· Metabolism > Energy metabolism > Sulfur metabolism.

References

1). Clinical significance of Interleukin 17 receptor E in diabetic nephropathy. International Immunopharmacology (PubMed: 37235960) [IF=5.6]

Application: IF/ICC Species: Human Sample: renal biopsy tissues

Fig. 4. Immunofluorescence double staining of IL-17RE and specific tubular/glomerular markers in renal biopsy tissues of patients with DN. IL-17RE was marked with FITC-conjugated secondary antibody; proximal tubular epithelial, distal tubular epithelial, and collecting duct cells were marked as AQP1, CD28k, and AQP3 with Cy3-conjugated secondary antibody, respectively; glomerular podocytes and endothelial and mesangial cells were marked as synaptopodin, CD31, and PDGFR-β with Cy3-conjugated secondary antibody, respectively. The white arrow indicates the positive staining area. NGAL, AQP1, CD28k, and AQP3: scale bar = 50 μm; synaptopodin, CD31, and PDGFR-β: scale bar = 25 μm. AQP, Aquaporin; CD28k, Calbindin D28k; PDGFR-β, platelet-derived growth factor receptors beta.

Application: IHC Species: Human Sample: renal biopsy tissues

Fig. 5. Pathological staining and protein expression levels in mouse kidney tissues. (A) H&E staining of the mouse kidneys. Scale bar = 50 μm. (B) PAS staining of mouse kidneys. Scale bar = 50 μm. (C) IHC assay of NGAL protein expression in the mouse kidneys. Scale bar = 50 μm. (D) IHC assay of IL-17RE protein expression in the mouse kidneys. Scale bar = 50 μm. The results represent the mean ± SD for six samples/group. *P < 0.05, ***P < 0.001 vs db/m. H&E, haematoxylin and eosin staining; PAS, periodic acid-Schiff staining; IHC, immunohistochemical staining.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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SQRDL Antibody - #DF12087