SQRDL Antibody - #DF12087

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Product: SQRDL Antibody
Catalog: DF12087
Description: Rabbit polyclonal antibody to SQRDL
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 50kd; 50kD(Calculated).
Uniprot: Q9Y6N5
RRID: AB_2844892

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(86%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SQRDL Antibody detects endogenous levels of total SQRDL.
RRID:
AB_2844892
Cite Format: Affinity Biosciences Cat# DF12087, RRID:AB_2844892.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

0610039J17Rik; 4930557M22Rik; CGI 44; CGI44; mitochondrial; PRO1975; SQRD_HUMAN; Sqrdl; Sulfide dehydrogenase like; Sulfide quinone reductase like; Sulfide quinone reductase-like (yeast); Sulfide:quinone oxidoreductase; Sulfide:quinone oxidoreductase, mitochondrial;

Immunogens

Immunogen:
Uniprot:

Q9Y6N5(SQOR_HUMAN) >>Visit HPA database.

Gene(ID):
SQOR(58472)
Sequence:
MVPLVAVVSGPRAQLFACLLRLGTQQVGPLQLHTGASHAARNHYEVLVLGGGSGGITMAARMKRKVGAENVAIVEPSERHFYQPIWTLVGAGAKQLSSSGRPTASVIPSGVEWIKARVTELNPDKNCIHTDDDEKISYRYLIIALGIQLDYEKIKGLPEGFAHPKIGSNYSVKTVEKTWKALQDFKEGNAIFTFPNTPVKCAGAPQKIMYLSEAYFRKTGKRSKANIIFNTSLGAIFGVKKYADALQEIIQERNLTVNYKKNLIEVRADKQEAVFENLDKPGETQVISYEMLHVTPPMSPPDVLKTSPVADAAGWVDVDKETLQHRRYPNVFGIGDCTNLPTSKTAAAVAAQSGILDRTISVIMKNQTPTKKYDGYTSCPLVTGYNRVILAEFDYKAEPLETFPFDQSKERLSMYLMKADLMPFLYWNMMLRGYWGGPAFLRKLFHLGMS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Sheep
88
Zebrafish
86
Xenopus
75
Bovine
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6N5 As Substrate

Site PTM Type Enzyme
K115 Acetylation
K125 Ubiquitination
K135 Acetylation
S137 Phosphorylation
Y151 Phosphorylation
K155 Ubiquitination
K165 Ubiquitination
K173 Acetylation
K173 Ubiquitination
K177 Ubiquitination
K180 Acetylation
K186 Acetylation
K186 Ubiquitination
T197 Phosphorylation
Y259 Phosphorylation
K260 Acetylation
K261 Ubiquitination
K320 Acetylation
T338 Phosphorylation
T342 Phosphorylation
S343 Phosphorylation
S361 Phosphorylation
Y395 Phosphorylation
S408 Phosphorylation
K409 Ubiquitination
S413 Phosphorylation
Y415 Phosphorylation
K443 Acetylation
S450 Phosphorylation

Research Backgrounds

Function:

Catalyzes the oxidation of hydrogen sulfide with the help of a quinone, such as ubiquinone, giving rise to thiosulfate and ultimately to sulfane (molecular sulfur) atoms. Requires an additional electron acceptor; can use sulfite, sulfide or cyanide (in vitro).

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the SQRD family.

Research Fields

· Metabolism > Energy metabolism > Sulfur metabolism.

References

1). Clinical significance of Interleukin 17 receptor E in diabetic nephropathy. International Immunopharmacology, 2023 (PubMed: 37235960) [IF=5.6]

Application: IF/ICC    Species: Human    Sample: renal biopsy tissues

Fig. 4. Immunofluorescence double staining of IL-17RE and specific tubular/glomerular markers in renal biopsy tissues of patients with DN. IL-17RE was marked with FITC-conjugated secondary antibody; proximal tubular epithelial, distal tubular epithelial, and collecting duct cells were marked as AQP1, CD28k, and AQP3 with Cy3-conjugated secondary antibody, respectively; glomerular podocytes and endothelial and mesangial cells were marked as synaptopodin, CD31, and PDGFR-β with Cy3-conjugated secondary antibody, respectively. The white arrow indicates the positive staining area. NGAL, AQP1, CD28k, and AQP3: scale bar = 50 μm; synaptopodin, CD31, and PDGFR-β: scale bar = 25 μm. AQP, Aquaporin; CD28k, Calbindin D28k; PDGFR-β, platelet-derived growth factor receptors beta.

Application: IHC    Species: Human    Sample: renal biopsy tissues

Fig. 5. Pathological staining and protein expression levels in mouse kidney tissues. (A) H&E staining of the mouse kidneys. Scale bar = 50 μm. (B) PAS staining of mouse kidneys. Scale bar = 50 μm. (C) IHC assay of NGAL protein expression in the mouse kidneys. Scale bar = 50 μm. (D) IHC assay of IL-17RE protein expression in the mouse kidneys. Scale bar = 50 μm. The results represent the mean ± SD for six samples/group. *P < 0.05, ***P < 0.001 vs db/m. H&E, haematoxylin and eosin staining; PAS, periodic acid-Schiff staining; IHC, immunohistochemical staining.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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