Product: RNase H1 Antibody
Catalog: DF12084
Description: Rabbit polyclonal antibody to RNase H1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 32 kDa; 32kD(Calculated).
Uniprot: O60930
RRID: AB_2844889

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 100ul $280 In stock
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Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
RNase H1 Antibody detects endogenous levels of total RNase H1.
RRID:
AB_2844889
Cite Format: Affinity Biosciences Cat# DF12084, RRID:AB_2844889.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

H1RNA; MGC108918; Ribonuclease H type II; RibonucleaseH1; RNaseH1; RNH1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O60930 RNH1_HUMAN:

Ubiquitous.

Sequence:
MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Xenopus
91
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60930 As Substrate

Site PTM Type Enzyme
S22 Phosphorylation
R52 Methylation
K60 Ubiquitination
K73 Ubiquitination
S74 Phosphorylation
S76 Phosphorylation
K119 Ubiquitination
R175 Methylation
K192 Ubiquitination
K198 Ubiquitination
K236 Ubiquitination
K241 Ubiquitination

Research Backgrounds

Function:

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the RNase H family.

Research Fields

· Genetic Information Processing > Replication and repair > DNA replication.

References

1). RNase H1 facilitates recombinase recruitment by degrading DNA–RNA hybrids during meiosis. Nucleic Acids Research, 2020 (PubMed: 37378420) [IF=14.9]

Application: WB    Species: Mouse    Sample:

Figure 1. Germ cell-specific Rnaseh1 knockout impairs mouse spermatogenesis and causes male infertility. (A) Schematic diagram for the construction of the Rnaseh1F/F and Stra8-Rnaseh1−/− mice. The mouse Rnaseh1 locus surrounding exons 2, 3 and 4 is shown in the wild-type allele panel. (B) The genotyping of the Rnaseh1+/+, Rnaseh1F/+, and Rnaseh1F/F mice. (C) The RNase H1 protein levels are decreased in Stra8-Rnaseh1−/−testes. Immunoblotting of RNase H1 is performed in Rnaseh1F/F and Stra8-Rnaseh1−/− testes. H3 and Tubulin serves as the loading control. (D) The quantification of RNase H1 protein levels in (C) (n = 5 independent experiments). Black dots indicate Rnaseh1F/F mice, and red dots indicate Stra8-Rnaseh1−/− mice. Data are presented as means ± SEM. two-tailed Student's t-test; **P < 0.01. (E and F) Germ cell-specific knockout of Rnaseh1 causes male infertility. The fertility assessment experiments are performed in Rnaseh1F/F and Stra8-Rnaseh1−/− male mice. Pregnancy rates (%) of plugged wild-type female mice after mating with Rnaseh1F/F and Stra8-Rnaseh1−/− male mice (n = 3 independent experiments) (E). Average litter sizes were observed with Rnaseh1F/F and Stra8-Rnaseh1−/− male mice (n = 3 independent experiments) (F). Black dots indicate Rnaseh1F/F mice, and red dots indicate Stra8-Rnaseh1−/− mice. Data are presented as means ± SEM. two-tailed Student's t-test; ***P < 0.001. (G) The Stra8-Rnaseh1−/− mouse testis is smaller than that of the Rnaseh1F/F mouse. (H) Ratio of testis weight/body weight in Rnaseh1F/F and Stra8-Rnaseh1−/− mice (n = 4 independent experiments). Black dots indicate Rnaseh1F/F mice and red dots indicate Stra8-Rnaseh1−/− mice. Data are presented as means ± SEM. two-tailed Student's t-test; *P < 0.05. (I) Histological analysis of the seminiferous tubules and caudal epididymis of the Rnaseh1F/F and Stra8-Rnaseh1−/− mice by hematoxylin and eosin staining. Arrowheads indicate dead cells. (J) Sperm counts from the cauda epididymis significantly decreased in the Stra8-Rnaseh1−/− mice compared with Rnaseh1F/F mice. (n = 5 independent experiments). Black dots indicate Rnaseh1F/F mice, and red dots indicate Stra8-Rnaseh1−/− mice.

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