OXCT1 Antibody - #DF12082

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Product: OXCT1 Antibody
Catalog: DF12082
Description: Rabbit polyclonal antibody to OXCT1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 56 kDa; 56kD(Calculated).
Uniprot: P55809
RRID: AB_2844887

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
OXCT1 Antibody detects endogenous levels of total OXCT1.
RRID:
AB_2844887
Cite Format: Affinity Biosciences Cat# DF12082, RRID:AB_2844887.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3 oxoacid CoA transferase 1; 3-oxoacid CoA-transferase 1; EC 2.8.3.5; OTTHUMP00000120012; OTTHUMP00000221550; OXCT; Oxct1; SCOT; Scot S; Scot-S; SCOT1_HUMAN; Somatic type succinyl CoA:3 oxoacid CoA transferase; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase; Succinyl CoA:3 ketoacid CoA transferase; Succinyl CoA:3 ketoacid coenzyme A transferase 1 mitochondrial; Succinyl CoA:3 oxoacid CoA transferase; Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial;

Immunogens

Immunogen:
Uniprot:

P55809(SCOT1_HUMAN) >>Visit HPA database.

Gene(ID):
OXCT1(5019)
Expression:
P55809 SCOT1_HUMAN:

Abundant in heart, followed in order by kidney, brain, and muscle, whereas in liver it is undetectable; also detectable in leukocytes and fibroblasts.

Sequence:
MAALKLLSSGLRLCASARGSGATWYKGCVCSFSTSAHRHTKFYTDPVEAVKDIPDGATVLVGGFGLCGIPENLIDALLKTGVKGLTAVSNNAGVDNFGLGLLLRSKQIKRMVSSYVGENAEFERQYLSGELEVELTPQGTLAERIRAGGAGVPAFYTPTGYGTLVQEGGSPIKYNKDGSVAIASKPREVREFNGQHFILEEAITGDFALVKAWKADRAGNVIFRKSARNFNLPMCKAAETTVVEVEEIVDIGAFAPEDIHIPQIYVHRLIKGEKYEKRIERLSIRKEGDGEAKSAKPGDDVRERIIKRAALEFEDGMYANLGIGIPLLASNFISPNITVHLQSENGVLGLGPYPRQHEADADLINAGKETVTILPGASFFSSDESFAMIRGGHVDLTMLGAMQVSKYGDLANWMIPGKMVKGMGGAMDLVSSAKTKVVVTMEHSAKGNAHKIMEKCTLPLTGKQCVNRIITEKAVFDVDKKKGLTLIELWEGLTVDDVQKSTGCDFAVSPKLMPMQQIAN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
100
Horse
92
Sheep
92
Xenopus
83
Chicken
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55809 As Substrate

Site PTM Type Enzyme
T86 Phosphorylation
S114 Phosphorylation
Y115 Phosphorylation
Y126 Phosphorylation
R146 Methylation
T157 Phosphorylation
T163 Phosphorylation
S170 Phosphorylation
K173 Ubiquitination
Y174 Phosphorylation
K176 Acetylation
K176 Ubiquitination
K185 Acetylation
K185 Ubiquitination
K225 Acetylation
S294 Phosphorylation
S381 Phosphorylation
K406 Acetylation
K418 Acetylation
K421 Acetylation
K421 Ubiquitination
S431 Phosphorylation
S432 Phosphorylation
T435 Phosphorylation
K446 Ubiquitination
K455 Acetylation
K463 Ubiquitination
K473 Acetylation
K473 Ubiquitination
K480 Acetylation
K480 Ubiquitination
K481 Acetylation
K482 Acetylation
S509 Phosphorylation

Research Backgrounds

Function:

Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.

Subcellular Location:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundant in heart, followed in order by kidney, brain, and muscle, whereas in liver it is undetectable; also detectable in leukocytes and fibroblasts.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the 3-oxoacid CoA-transferase family.

Research Fields

· Metabolism > Lipid metabolism > Synthesis and degradation of ketone bodies.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Carbohydrate metabolism > Butanoate metabolism.

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