MAT2B Antibody - #DF12080

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Product: MAT2B Antibody
Catalog: DF12080
Description: Rabbit polyclonal antibody to MAT2B
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 38 kDa; 38kD(Calculated).
Uniprot: Q9NZL9
RRID: AB_2844885

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
MAT2B Antibody detects endogenous levels of total MAT2B.
RRID:
AB_2844885
Cite Format: Affinity Biosciences Cat# DF12080, RRID:AB_2844885.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1110064C04Rik; 2410018D16Rik; AI182287; AU022853; Beta regulatory subunit of methionine adenosyltransferase; dTDP 4 keto 6 deoxy D glucose 4 reductase; DTDP-4-keto-6-deoxy-D-glucose 4-reductase; MAT II; MAT II beta; Mat2b; MAT2B_HUMAN; MATIIbeta; Methionine adenosyltransferase 2 beta subunit; Methionine adenosyltransferase 2 subunit beta; Methionine adenosyltransferase II beta; MGC12237; MSTP045; Nbla02999; OTTMUSP00000005600; Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase; Putative protein product of Nbla02999; RP23-382C18.2; SDR23E1; Short chain dehydrogenase/reductase family 23E member 1; TGR;

Immunogens

Immunogen:
Uniprot:

Q9NZL9(MAT2B_HUMAN) >>Visit HPA database.

Gene(ID):
MAT2B(27430)
Expression:
Q9NZL9 MAT2B_HUMAN:

Widely expressed.

Sequence:
MVGREKELSIHFVPGSCRLVEEEVNIPNRRVLVTGATGLLGRAVHKEFQQNNWHAVGCGFRRARPKFEQVNLLDSNAVHHIIHDFQPHVIVHCAAERRPDVVENQPDAASQLNVDASGNLAKEAAAVGAFLIYISSDYVFDGTNPPYREEDIPAPLNLYGKTKLDGEKAVLENNLGAAVLRIPILYGEVEKLEESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVATVCRQLAEKRMLDPSIKGTFHWSGNEQMTKYEMACAIADAFNLPSSHLRPITDSPVLGAQRPRNAQLDCSKLETLGIGQRTPFRIGIKESLWPFLIDKRWRQTVFH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
83
Zebrafish
50
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NZL9 As Substrate

Site PTM Type Enzyme
Ubiquitination
S9 Phosphorylation
T34 Phosphorylation
T37 Phosphorylation
K46 Acetylation
K46 Ubiquitination
K168 Ubiquitination
Y186 Phosphorylation
K191 Ubiquitination
K209 Ubiquitination
S210 Phosphorylation
K225 Ubiquitination
K245 Ubiquitination
S273 Phosphorylation
T280 Phosphorylation
S282 Phosphorylation
K299 Acetylation
K299 Ubiquitination
T309 Phosphorylation
K316 Ubiquitination
S318 Phosphorylation
K326 Ubiquitination

Research Backgrounds

Function:

Regulatory subunit of S-adenosylmethionine synthetase 2, an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates MAT2A catalytic activity by changing its kinetic properties, increasing its affinity for L-methionine. Can bind NADP (in vitro).

Tissue Specificity:

Widely expressed.

Subunit Structure:

Heterotrimer; composed of a catalytic MAT2A homodimer that binds one regulatory MAT2B chain. Heterohexamer; composed of a central, catalytic MAT2A homotetramer flanked on either side by a regulatory MAT2B chain. NADP binding increases the affinity for MAT2A.

Family&Domains:

Belongs to the dTDP-4-dehydrorhamnose reductase family. MAT2B subfamily.

Research Fields

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

 

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