LGALS3BP Antibody - #DF12079

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Product: LGALS3BP Antibody
Catalog: DF12079
Description: Rabbit polyclonal antibody to LGALS3BP
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 65-90 kDa; 65kD(Calculated).
Uniprot: Q08380
RRID: AB_2844884

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(88%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
LGALS3BP Antibody detects endogenous levels of total LGALS3BP.
RRID:
AB_2844884
Cite Format: Affinity Biosciences Cat# DF12079, RRID:AB_2844884.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

90K; 90K MAC 2 BP; Basement membrane autoantigen p105; BTBD17B; Galectin 3 binding protein; Galectin-3-binding protein; L3 antigen; Lectin galactoside binding soluble 3 binding protein; Lectin galactoside-binding soluble 3-binding protein; LG3BP_HUMAN; LGALS3BP; M2BP; Mac 2 binding protein; Mac 2 BP; Mac-2 BP; Mac-2-binding protein; Mac2 BP; MAC2BP; Serum protein 90K; TANGO10B; Transport and golgi organization 10 homolog B; Tumor associated antigen 90K; Tumor-associated antigen 90K;

Immunogens

Immunogen:
Uniprot:

Q08380(LG3BP_HUMAN) >>Visit HPA database.

Gene(ID):
LGALS3BP(3959)
Expression:
Q08380 LG3BP_HUMAN:

Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.

Sequence:
MTPPRLFWVWLLVAGTQGVNDGDMRLADGGATNQGRVEIFYRGQWGTVCDNLWDLTDASVVCRALGFENATQALGRAAFGQGSGPIMLDEVQCTGTEASLADCKSLGWLKSNCRHERDAGVVCTNETRSTHTLDLSRELSEALGQIFDSQRGCDLSISVNVQGEDALGFCGHTVILTANLEAQALWKEPGSNVTMSVDAECVPMVRDLLRYFYSRRIDITLSSVKCFHKLASAYGARQLQGYCASLFAILLPQDPSFQMPLDLYAYAVATGDALLEKLCLQFLAWNFEALTQAEAWPSVPTDLLQLLLPRSDLAVPSELALLKAVDTWSWGERASHEEVEGLVEKIRFPMMLPEELFELQFNLSLYWSHEALFQKKTLQALEFHTVPFQLLARYKGLNLTEDTYKPRIYTSPTWSAFVTDSSWSARKSQLVYQSRRGPLVKYSSDYFQAPSDYRYYPYQSFQTPQHPSFLFQDKRVSWSLVYLPTIQSCWNYGFSCSSDELPVLGLTKSGGSDRTIAYENKALMLCEGLFVADVTDFEGWKAAIPSALDTNSSKSTSSFPCPAGHFNGFRTVIRPFYLTNSSGVD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Pig
88
Horse
88
Rabbit
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q08380 As Substrate

Site PTM Type Enzyme
Y41 Phosphorylation
N69 N-Glycosylation
K110 Ubiquitination
N125 N-Glycosylation
N192 N-Glycosylation
K229 Ubiquitination
S256 Phosphorylation
N398 N-Glycosylation
K405 Ubiquitination
S411 Phosphorylation
T413 Phosphorylation
S415 Phosphorylation
K427 Ubiquitination
S428 Phosphorylation
Y432 Phosphorylation
S434 Phosphorylation
K441 Ubiquitination
S443 Phosphorylation
S444 O-Glycosylation
S444 Phosphorylation
Y446 Phosphorylation
Y458 Phosphorylation
T463 Phosphorylation
K474 Ubiquitination
N551 N-Glycosylation
K554 Ubiquitination
N580 N-Glycosylation

Research Backgrounds

Function:

Promotes integrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.

Subcellular Location:

Secreted. Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.

Subunit Structure:

Homodimers and homomultimers. The multimers form ring-like structures with a diameter of 30-40 nm. Binds LGALS1 and LGALS3. Binds ITGB1, COL4A1, COL5A1, COL6A1, FN1 and NID. Interacts with the gamma-tubulin ring complex (gamma-TuRC), composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6. The unglycosylated form interacts with PDE4DIP isoform 13/MMG8/SMYLE; this interaction may connect a pericentrosomal complex, made of AKAP9, CDK5RAP2, EB1/MAPRE1 and PDE4DIP, to the gamma-tubulin ring complex (gamma-TuRC) to promote microtubule assembly and acetylation.

References

1). Overexpression of CD59 inhibits apoptosis of T-acute lymphoblastic leukemia via AKT/Notch1 signaling pathway. Cancer Cell International, 2019 (PubMed: 30636930) [IF=5.8]

Application: WB    Species: human    Sample: Jurkat cells

Fig.?4?|The activation of AKT, STAT5 and Notch1 signaling pathway are involve in the regulation of apoptosis by CD59 in Jurkat cells. The expression of CD59 (a), apoptosis-related proteins (c) and key proteins in signaling pathways (e) detected by western blot. Quantitative analysis of the protein expression of CD59 (b), apoptosis-related proteins (d) and key proteins in signaling pathways (f, g). All experiments were performed three times. *P?

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