IST1 Antibody - #DF12077

(2)  
Product: IST1 Antibody
Catalog: DF12077
Description: Rabbit polyclonal antibody to IST1
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 40 kDa; 40kD(Calculated).
Uniprot: P53990
RRID: AB_2844882

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
IST1 Antibody detects endogenous levels of total IST1.
RRID:
AB_2844882
Cite Format: Affinity Biosciences Cat# DF12077, RRID:AB_2844882.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hIST1; Increased sodium tolerance 1; Increased sodium tolerance 1 homolog; IST1 homolog; IST1_HUMAN; KIAA0174; MAPK activating protein PM28; MGC117220; MGC128433; Putative MAPK-activating protein PM28;

Immunogens

Immunogen:
Uniprot:

P53990(IST1_HUMAN) >>Visit HPA database.

Gene(ID):
IST1(9798)
Sequence:
MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIPATPPSYESVDDINADKNISSAQIVGPGPKPEASAKLPSRPADNYDNFVLPELPSVPDTLPTASAGASTSASEDIDFDDLSRRFEELKKKT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
60
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P53990 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
K7 Ubiquitination
K35 Ubiquitination
K38 Ubiquitination
Y43 Phosphorylation
K48 Ubiquitination
S88 Phosphorylation
S112 Phosphorylation
K118 Ubiquitination
K127 Ubiquitination
K134 Ubiquitination
R147 Methylation
K151 Ubiquitination
S153 Phosphorylation
K159 Ubiquitination
K205 Ubiquitination
K206 Ubiquitination
S214 Phosphorylation
K249 Sumoylation
T276 Phosphorylation
S279 Phosphorylation
S293 Phosphorylation
S294 Phosphorylation
K303 Ubiquitination
S307 Phosphorylation
K309 Ubiquitination
T335 Phosphorylation
S337 Phosphorylation
K361 Ubiquitination

Research Backgrounds

Function:

ESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST.

Subcellular Location:

Cytoplasmic vesicle. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Midbody. Nucleus envelope.
Note: Localizes to centrosome and midbody of dividing cells (PubMed:19129480, PubMed:19129479, PubMed:20719964). Colocalized with SPART to the ends of Flemming bodies during cytokinesis (PubMed:20719964). Localizes to the nuclear envelope during late anaphase (PubMed:26040712).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with CHMP1A, CHMP1B, VPS4A and VTA1. Interacts with SPAST, STAMBP, and USP8. May interact with VPS37B. May associate with the ESCRT-I complex. Interacts with MITD1, in competition with VSP4. Interacts with SPART (via MIT domain); leading to the recruitment of SPART to midbodies. Interacts with SPAST.

Family&Domains:

Belongs to the IST1 family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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