HEY1 Antibody - #DF12076

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Product: HEY1 Antibody
Catalog: DF12076
Description: Rabbit polyclonal antibody to HEY1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 32-34 kDa; 33kD(Calculated).
Uniprot: Q9Y5J3
RRID: AB_2844881

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(83%), Horse(100%), Sheep(83%), Rabbit(83%), Dog(83%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
HEY1 Antibody detects endogenous levels of total HEY1.
RRID:
AB_2844881
Cite Format: Affinity Biosciences Cat# DF12076, RRID:AB_2844881.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Basic helix loop helix protein OAF1; bHLHb31; Cardiovascular helix loop-helix factor 2; Cardiovascular helix-loop-helix factor 2; CHF-2; CHF2; Class B basic helix-loop-helix protein 31; Hairy and enhancer of split related protein 1; Hairy and enhancer of split-related protein 1; Hairy enhancer-of-split related with YRPW motif protein 1; Hairy related transcription factor 1; Hairy-related transcription factor 1; Hairy/enhancer of split related with YRPW motif 1; Hairy/enhancer-of-split related with YRPW motif protein 1; HERP2; HES related repressor protein 1; HES-related repressor protein 1; HES-related repressor protein 2; HESR-1; HESR1; HEY 1; hey1; HEY1_HUMAN; hHRT1; HRT-1; HRT1; OAF1;

Immunogens

Immunogen:
Uniprot:

Q9Y5J3(HEY1_HUMAN) >>Visit HPA database.

Gene(ID):
HEY1(23462)
Expression:
Q9Y5J3 HEY1_HUMAN:

Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.

Sequence:
MKRAHPEYSSSDSELDETIEVEKESADENGNLSSALGSMSPTTSSQILARKRRRGIIEKRRRDRINNSLSELRRLVPSAFEKQGSAKLEKAEILQMTVDHLKMLHTAGGKGYFDAHALAMDYRSLGFRECLAEVARYLSIIEGLDASDPLRVRLVSHLNNYASQREAASGAHAGLGHIPWGTVFGHHPHIAHPLLLPQNGHGNAGTTASPTEPHHQGRLGSAHPEAPALRAPPSGSLGPVLPVVTSASKLSPPLLSSVASLSAFPFSFGSFHLLSPNALSPSAPTQAANLGKPYRPWGTEIGAF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
83
Sheep
83
Dog
83
Chicken
83
Rabbit
83
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5J3 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
S11 Phosphorylation
S13 Phosphorylation
S25 Phosphorylation
S45 Phosphorylation
K59 Acetylation
S68 Phosphorylation
S78 Phosphorylation
T97 Phosphorylation
Y161 Phosphorylation
S163 Phosphorylation
S246 Phosphorylation

Research Backgrounds

Function:

Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Downstream effector of Notch signaling required for cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY2 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3. Involved in the regulation of liver cancer cells self-renewal.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.

Subunit Structure:

Self-associates. Interacts with HES1 and HEYL. Interacts with HDAC1, NCOR1 and SIN3A. Interacts with GATA4 and GATA6. Interacts with CCDC89/BOIP.

Family&Domains:

Belongs to the HEY family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

References

1). Inhibition of the Notch1 Pathway Promotes the Effects of Nucleus Pulposus Cell-Derived Exosomes on the Differentiation of Mesenchymal Stem Cells into Nucleus Pulposus-Like Cells in Rats. Stem Cells International, 2019 (PubMed: 31249601) [IF=4.3]

Application: WB    Species: rat    Sample: MSCs

Figure 3|: Expression of NPC markers and Notch pathway in NPC exosome-treated MSCs. (a) Gene expression of NPC markers (collagen II,Aggrecan, and Sox9) was significantly upregulated in exosome-treated MSCs in 7, 14, and 21 days. (b) Expression of related protein was normalized to GAPDH. (c) Expression of Notch pathway-related genes in exosome-treated MSCs was detected by qRT-PCR. (d)Western blot analysis of Notch pathway-related protein in exosome-treated MSCs. All data were showed as mean ± SD. N = 3. ∗P < 0 05, ∗∗P < 0 01, and ∗∗∗P < 0 001.
2). γ‐secretase inhibitors suppress IL‐20‐mediated osteoclastogenesis via Notch signaling and are affected by Notch2 in vitro. SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2022 (PubMed: 35384009) [IF=3.7]

Application: WB    Species: Human    Sample:

FIGURE 2 20 ng/mL IL-20 treated the pre-osteoclasts for 48 h meanwhile control groups were treated with 0 ng/mL IL-20. Detected mRNA and analysed by GO Analysis and Pathway Analysis. (A) Bubble chart analysis based on KEGG databases. (B) Gene Set Enrichment Analysis of osteoclast differentiation with control groups and IL-20 treat groups. (C) Gene heatmap showed four classical gene were enriched to Notch signal pathway (Red – up regulated and green – down regulated). (D-E) The expression of Notch1 and Notch2 mRNA with different concentration of IL-20 stimulation. Analysed by one-way ANOVA with Tukey's post hoc analysis with three independent experiments. *P < .05 vs the control group; ns, not significant. (F) mRNA expression of Notch1, Notch2, Hes1 and Hey1 at 20 ng/mL IL-20 stimulation using by qRT-PCR and analysed by Student's t test. Bars represent mean ± SEM from three independent experiments. *P < .05 vs the control group; ns, not significant. (G-H) Western blotting data and analysis of Notch signal pathway. Student's t test was used to detect difference. Bars represent mean ± SEM from three independent experiments. *P < .05 vs the control group; ns, not significant

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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