Product: eIF4A3 Antibody
Catalog: DF12071
Description: Rabbit polyclonal antibody to eIF4A3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 47 kDa; 47kD(Calculated).
Uniprot: P38919
RRID: AB_2844876

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
eIF4A3 Antibody detects endogenous levels of total eIF4A3.
RRID:
AB_2844876
Cite Format: Affinity Biosciences Cat# DF12071, RRID:AB_2844876.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DDX48; DEAD box protein 48; eIF-4A-III; eIF4A-III; EIF4A3; eIF4AIII; Eukaryotic initiation factor 4A-III; Eukaryotic initiation factor 4A-like NUK-34; Eukaryotic translation initiation factor 4A isoform 3; hNMP 265; IF4A3_HUMAN; NMP 265; NMP265; Nuclear matrix protein 265; NUK34;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P38919 IF4A3_HUMAN:

Ubiquitously expressed.

Sequence:
MATTATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P38919 As Substrate

Site PTM Type Enzyme
M1 Acetylation
A2 Acetylation
T3 Phosphorylation
T4 Phosphorylation
T6 Phosphorylation
T9 Phosphorylation
S10 Phosphorylation
S12 Phosphorylation
R14 Methylation
K19 Sumoylation
K19 Ubiquitination
S31 Phosphorylation
Y54 Phosphorylation
Y56 Phosphorylation
K60 Acetylation
K60 Methylation
K60 Sumoylation
K60 Ubiquitination
K70 Ubiquitination
S84 Phosphorylation
Y132 Phosphorylation
K152 Ubiquitination
Y155 Phosphorylation
T163 Phosphorylation P24941 (CDK2)
K182 Ubiquitination
K195 Ubiquitination
K198 Acetylation
K198 Ubiquitination
Y202 Phosphorylation
Y205 Phosphorylation
S218 Phosphorylation
T247 Phosphorylation
K289 Ubiquitination
K296 Acetylation
K296 Ubiquitination
K314 Ubiquitination
K321 Acetylation
K321 Ubiquitination
K374 Ubiquitination
K382 Acetylation
K382 Ubiquitination

Research Backgrounds

Function:

ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. Involved in craniofacial development.

Subcellular Location:

Nucleus. Nucleus speckle. Cytoplasm.
Note: Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments of neurons. Colocalizes with STAU1 and FMR1 in dendrites (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Identified in the spliceosome C complex. Core component of the mRNA splicing-dependent exon junction complex (EJC); the core complex contains CASC3, EIF4A3, MAGOH or MAGOHB, and RBM8A. Interacts with CASC3, MAGOH, NXF1, RBM8A and ALYREF/THOC4. May interact with NOM1. Interacts with POLDIP3. Interacts with CWC22 and PRPF19 in an RNA-independent manner. Direct interaction with CWC22 is mediated by the helicase C-terminal domain. Full interaction with CWC22 occurs only when EIF4A3 is not part of the EJC and prevents EIF4A3 binding to RNA. Identified in a complex composed of the EJC core, UPF3B and UPF2. The EJC core can also interact with UPF3A (in vitro). Interacts with NCBP3.

Family&Domains:

Belongs to the DEAD box helicase family. eIF4A subfamily.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Genetic Information Processing > Transcription > Spliceosome.

References

1). The Combined Effects of circRNA Methylation Promote Pulmonary Fibrosis. AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2022 (PubMed: 35213290) [IF=6.4]

Application: WB    Species: Human    Sample: lung fibroblasts

Figure 4. As the target proteins of hsa_circ_0000672 and hsa_circ_0005654, eIF4A3 promotes the activation, migration and activity of human lung fibroblasts. PCR was used to detect the expression of hsa_circ_0000672 and hsa_circ_0005654 in human lung fibroblasts at different time points. The data are presented as the mean ± SD, n = 3. (B) In situ hybridization showed that hsa_circ_0000672 and hsa_circ_0005654 were present in the cytoplasm. Scale bars, 20 μm. (C) Bioinformatics analysis showed that hsa_circ_0000672 and hsa_circ_0005654 pre-mRNAs have a common RNA binding protein (RBP). (D) The RIP test was used to detect the interaction of hsa_circ_0000672 and hsa_circ_0005654 with eIF4A3. (E and F) Knockdown efficiency of EIF4A3. The data are presented as the mean ± SD, n = 3. *P 

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