ABHD5 Antibody - #DF12065

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Product: ABHD5 Antibody
Catalog: DF12065
Description: Rabbit polyclonal antibody to ABHD5
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 39 kDa; 39kD(Calculated).
Uniprot: Q8WTS1
RRID: AB_2844870

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(90%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ABHD5 Antibody detects endogenous levels of total ABHD5.
RRID:
AB_2844870
Cite Format: Affinity Biosciences Cat# DF12065, RRID:AB_2844870.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1-acylglycerol-3-phosphate O-acyltransferase ABHD5; ABHD5; ABHD5_HUMAN; Abhydrolase domain containing 5; Abhydrolase domain containing protein 5; Abhydrolase domain-containing protein 5; CDS; CGI 58; CGI58; CGI58 protein; IECN2; Lipid droplet-binding protein CGI-58; MGC8731; NCIE2;

Immunogens

Immunogen:
Uniprot:

Q8WTS1(ABHD5_HUMAN) >>Visit HPA database.

Gene(ID):
ABHD5(51099)
Expression:
Q8WTS1 ABHD5_HUMAN:

Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).

Sequence:
MAAEEEEVDSADTGERSGWLTGWLPTWCPTSISHLKEAEEKMLKCVPCTYKKEPVRISNGNKIWTLKFSHNISNKTPLVLLHGFGGGLGLWALNFGDLCTNRPVYAFDLLGFGRSSRPRFDSDAEEVENQFVESIEEWRCALGLDKMILLGHNLGGFLAAAYSLKYPSRVNHLILVEPWGFPERPDLADQDRPIPVWIRALGAALTPFNPLAGLRIAGPFGLSLVQRLRPDFKRKYSSMFEDDTVTEYIYHCNVQTPSGETAFKNMTIPYGWAKRPMLQRIGKMHPDIPVSVIFGARSCIDGNSGTSIQSLRPHSYVKTIAILGAGHYVYADQPEEFNQKVKEICDTVD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
100
Bovine
90
Xenopus
70
Chicken
70
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WTS1 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K36 Ubiquitination
K44 Ubiquitination
K62 Ubiquitination
Y236 Phosphorylation
K283 Ubiquitination

Research Backgrounds

Function:

Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfert of an acyl group on a lysophosphatidic acid. Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors (By similarity). Functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. Regulates lipid droplet fusion (By similarity).

Subcellular Location:

Cytoplasm. Lipid droplet. Cytoplasm>Cytosol.
Note: Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).

Subunit Structure:

Interacts with ADRP, PLIN and PNPLA2. Interacts with PLIN5; promotes interaction with PNPLA2 (By similarity).

Family&Domains:

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.

Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.

Research Fields

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

References

1). Association between the levels of CGI‑58 and lipoprotein lipase in the placenta of patients with preeclampsia. Experimental and Therapeutic Medicine (PubMed: 34466143) [IF=2.7]

Application: IHC    Species: Human    Sample: placental tissue

Figure 3 Immunohistochemical examination of CGI-58 in the (A) preeclampsia and (B) control (normal pregnant) groups. Samples were considered CGI-58-positive when brown-yellow granules were detected in the cytoplasm and cell membrane (red row). Magnification, x400. Scale bar, 50-µm. CGI-58, comparative gene identification-58.

Application: WB    Species: Human    Sample: placental tissue

Figure 5 Representative expression of CGI-58 and LPL in control (normal pregnant, column 1, 2, 3) and preeclampsia (column 4, 5, 6) groups evaluated by western blotting. CGI-58, comparative gene identification-58; LPL, lipoprotein lipase.

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