ABHD5 Antibody | Affinity Biosciences

IHC
Cites

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DF12065 at 1/100 staining Rat muscle tissue by IHC-P.

Figure 3 Immunohistochemical examination of CGI-58 in the (A) preeclampsia and (B) control (normal pregnant) groups.

Figure 5 Representative expression of CGI-58 and LPL in control (normal pregnant, column 1, 2, 3) and preeclampsia (column 4, 5, 6) groups evaluated by western blotting.

Product:	ABHD5 Antibody
Catalog:	DF12065
Description:	Rabbit polyclonal antibody to ABHD5
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	39 kDa; 39kD(Calculated).
Uniprot:	Q8WTS1
RRID:	AB_2844870

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(90%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ABHD5 Antibody detects endogenous levels of total ABHD5.

RRID:

AB_2844870
Cite Format: Affinity Biosciences Cat# DF12065, RRID:AB_2844870.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1-acylglycerol-3-phosphate O-acyltransferase ABHD5; ABHD5; ABHD5_HUMAN; Abhydrolase domain containing 5; Abhydrolase domain containing protein 5; Abhydrolase domain-containing protein 5; CDS; CGI 58; CGI58; CGI58 protein; IECN2; Lipid droplet-binding protein CGI-58; MGC8731; NCIE2;

Immunogens

Immunogen:

Uniprot:

Q8WTS1(ABHD5_HUMAN) >>Visit HPA database.

Gene(ID):

ABHD5(51099)

Expression:

Q8WTS1 ABHD5_HUMAN:

Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).

Sequence:

Q8WTS1 ABHD5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAEEEEVDSADTGERSGWLTGWLPTWCPTSISHLKEAEEKMLKCVPCTYKKEPVRISNGNKIWTLKFSHNISNKTPLVLLHGFGGGLGLWALNFGDLCTNRPVYAFDLLGFGRSSRPRFDSDAEEVENQFVESIEEWRCALGLDKMILLGHNLGGFLAAAYSLKYPSRVNHLILVEPWGFPERPDLADQDRPIPVWIRALGAALTPFNPLAGLRIAGPFGLSLVQRLRPDFKRKYSSMFEDDTVTEYIYHCNVQTPSGETAFKNMTIPYGWAKRPMLQRIGKMHPDIPVSVIFGARSCIDGNSGTSIQSLRPHSYVKTIAILGAGHYVYADQPEEFNQKVKEICDTVD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

100

Rabbit

100

Bovine

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WTS1 As Substrate

Q8WTS1

Site	PTM Type	Source
A2	Acetylation	Uniprot
K36	Ubiquitination	Uniprot
K44	Ubiquitination	Uniprot
K62	Ubiquitination	Uniprot
Y236	Phosphorylation	Uniprot
K283	Ubiquitination	Uniprot

Research Backgrounds

Q8WTS1_ABHD5_HUMAN

Function:

Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfert of an acyl group on a lysophosphatidic acid. Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors (By similarity). Functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. Regulates lipid droplet fusion (By similarity).

Subcellular Location:

Cytoplasm. Lipid droplet. Cytoplasm>Cytosol.
Note: Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA (By similarity).

Tissue Specificity:

Subunit Structure:

Interacts with ADRP, PLIN and PNPLA2. Interacts with PLIN5; promotes interaction with PNPLA2 (By similarity).

Family&Domains:

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.

Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.

Research Fields

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

References

1). Association between the levels of CGI‑58 and lipoprotein lipase in the placenta of patients with preeclampsia. Experimental and Therapeutic Medicine, 2021 (PubMed: 34466143) [IF=2.7]

Application: IHC Species: Human Sample: placental tissue

Figure 3 Immunohistochemical examination of CGI-58 in the (A) preeclampsia and (B) control (normal pregnant) groups. Samples were considered CGI-58-positive when brown-yellow granules were detected in the cytoplasm and cell membrane (red row). Magnification, x400. Scale bar, 50-µm. CGI-58, comparative gene identification-58.

Application: WB Species: Human Sample: placental tissue

Figure 5 Representative expression of CGI-58 and LPL in control (normal pregnant, column 1, 2, 3) and preeclampsia (column 4, 5, 6) groups evaluated by western blotting. CGI-58, comparative gene identification-58; LPL, lipoprotein lipase.

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ABHD5 Antibody - #DF12065