Transgelin 2 Antibody - #DF12053
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| Product: | Transgelin 2 Antibody |
| Catalog: | DF12053 |
| Description: | Rabbit polyclonal antibody to Transgelin 2 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 22 kDa; 22kD(Calculated). |
| Uniprot: | P37802 |
| RRID: | AB_2844858 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Transgelin 2 Antibody detects endogenous levels of total Transgelin 2.
RRID:
AB_2844858
Cite Format: Affinity Biosciences Cat# DF12053, RRID:AB_2844858.
Cite Format: Affinity Biosciences Cat# DF12053, RRID:AB_2844858.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
CDABP0035; epididymis tissue protein Li 7e; HA1756; KIAA0120; SM22-alpha homolog; TAGL2_HUMAN; Tagln2; Transgelin-2;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- P37802 TAGL2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MANRGPAYGLSREVQQKIEKQYDADLEQILIQWITTQCRKDVGRPQPGRENFQNWLKDGTVLCELINALYPEGQAPVKKIQASTMAFKQMEQISQFLQAAERYGINTTDIFQTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKKSKENPRNFSDNQLQEGKNVIGLQMGTNRGASQAGMTGYGMPRQIL
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
82
Xenopus
75
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P37802 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| R4 | Methylation | Uniprot | |
| Y8 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| K17 | Acetylation | Uniprot | |
| K17 | Ubiquitination | Uniprot | |
| K20 | Acetylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| K57 | Sumoylation | Uniprot | |
| T60 | Phosphorylation | Uniprot | |
| K78 | Acetylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K79 | Sumoylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| S83 | Phosphorylation | O94921 (CDK14) | Uniprot |
| T84 | Phosphorylation | Uniprot | |
| K88 | Acetylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| T128 | Phosphorylation | Uniprot | |
| R139 | Methylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| K153 | Acetylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| S163 | Phosphorylation | O94921 (CDK14) , P17612 (PRKACA) | Uniprot |
| K171 | Acetylation | Uniprot | |
| K171 | Sumoylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| T180 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| R182 | Methylation | Uniprot | |
| S185 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T190 | Phosphorylation | Uniprot | |
| Y192 | Phosphorylation | Uniprot | |
| R196 | Methylation | Uniprot |
Research Backgrounds
Tissue Specificity:
Expressed in epididymis (at protein level).
Family&Domains:
Belongs to the calponin family.
References
1). Comparative proteomics identify HSP90A, STIP1 and TAGLN‑2 in serum extracellular vesicles as potential circulating biomarkers for human adenomyosis. Experimental and Therapeutic Medicine, 2022
(PubMed: 35495589)
[IF=2.7]
Application: WB Species: human Sample: T‑AMEVs and B‑AMEVs
Figure 4. | HSP90A, STIP1 and TAGLN‑2 are expressed in B‑AMEVs and T‑AMEVs. (D and E) HSP90A, STIP1 and TAGLN‑2 were present in T‑AMEVs and B‑AMEVs from patients with adenomyosis,but not in those of patients in the control group.
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For Research Use Only.
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