Product: SGTA Antibody
Catalog: DF12036
Description: Rabbit polyclonal antibody to SGTA
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 34-37 kDa; 34kD(Calculated).
Uniprot: O43765
RRID: AB_2844841

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SGTA Antibody detects endogenous levels of total SGTA.
RRID:
AB_2844841
Cite Format: Affinity Biosciences Cat# DF12036, RRID:AB_2844841.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Alpha SGT; Alpha-SGT; AlphaSGT; hSGT; SGT; Sgt1; SGTA; SGTA_HUMAN; Small glutamine rich protein with tetratricopeptide repeats 1; Small glutamine rich tetratricopeptide repeat containing protein alpha; Small glutamine rich tetratricopeptide repeat TPR containing alpha; Small glutamine rich tetratricopeptide repeat-containing protein; Small glutamine-rich tetratricopeptide repeat-containing protein alpha; Stg; UBP; Vpu binding protein; Vpu-binding protein;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O43765 SGTA_HUMAN:

Ubiquitous.

Sequence:
MDNKKRLAYAIIQFLHDQLRHGGLSSDAQESLEVAIQCLETAFGVTVEDSDLALPQTLPEIFEAAATGKEMPQDLRSPARTPPSEEDSAEAERLKTEGNEQMKVENFEAAVHFYGKAIELNPANAVYFCNRAAAYSKLGNYAGAVQDCERAICIDPAYSKAYGRMGLALSSLNKHVEAVAYYKKALELDPDNETYKSNLKIAELKLREAPSPTGGVGSFDIAGLLNNPGFMSMASNLMNNPQIQQLMSGMISGGNNPLGTPGTSPSQNDLASLIQAGQQFAQQMQQQNPELIEQLRSQIRSRTPSASNDDQQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Horse
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43765 As Substrate

Site PTM Type Enzyme
K4 Acetylation
Y9 Phosphorylation
R76 Methylation
S77 Phosphorylation
T81 Phosphorylation
S84 Phosphorylation
K95 Ubiquitination
K103 Ubiquitination
K116 Ubiquitination
C129 S-Nitrosylation
K137 Acetylation
K137 Ubiquitination
Y141 Phosphorylation
C148 S-Nitrosylation
C153 S-Nitrosylation
Y158 Phosphorylation
S159 Phosphorylation
K160 Acetylation
K160 Ubiquitination
Y162 Phosphorylation
S171 Phosphorylation
K174 Ubiquitination
K183 Ubiquitination
K184 Ubiquitination
K196 Ubiquitination
K200 Ubiquitination
K205 Ubiquitination
S297 Phosphorylation
S301 Phosphorylation
T303 Phosphorylation
S305 Phosphorylation
S307 Phosphorylation

Research Backgrounds

Function:

Co-chaperone that binds misfolded and hydrophobic patches-containing client proteins in the cytosol. Mediates their targeting to the endoplasmic reticulum but also regulates their sorting to the proteasome when targeting fails. Functions in tail-anchored/type II transmembrane proteins membrane insertion constituting with ASNA1 and the BAG6 complex a targeting module. Functions upstream of the BAG6 complex and ASNA1, binding more rapidly the transmembrane domain of newly synthesized proteins. It is also involved in the regulation of the endoplasmic reticulum-associated misfolded protein catabolic process via its interaction with BAG6: collaborates with the BAG6 complex to maintain hydrophobic substrates in non-ubiquitinated states. Competes with RNF126 for interaction with BAG6, preventing the ubiquitination of client proteins associated with the BAG6 complex. Binds directly to HSC70 and HSP70 and regulates their ATPase activity.

(Microbial infection) In case of infection by polyomavirus, involved in the virus endoplasmic reticulum membrane penetration and infection via interaction with DNAJB12, DNAJB14 and HSPA8/Hsc70.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Co-localizes with HSP90AB1 in the cytoplasm. Increased nuclear accumulation seen during cell apoptosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homodimer. Homooligomer (By similarity). Interacts with DNAJC5 and DNAJC5B. Interacts (via TPR repeats) with HSP90AA1. Interacts (via Gln-rich region) with SLC2A1. Interacts with HSP90AB1. Interacts (via TPR repeats) with HSPA8/Hsc70; the interaction is direct. Interacts with BAG6 (via ubiquitin-like domain); interaction prevents interaction between BAG6 and RNF126. Forms a multiprotein complex, at least composed of DNAJB12, DNAJB14, HSPA8/Hsc70 and SGTA; interaction with DNAJB14 and HSPA8/Hsc70 is direct.

(Microbial infection) Interacts with Vpu and Gag from HIV-1.

(Microbial infection) Interacts with SARS-CoV accessory protein 7a.

Family&Domains:

The second tetratricopeptide repeat (TPR 2) mediates the interaction with SARS-CoV accessory protein 7a.

Belongs to the SGT family.

Restrictive clause

 

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