Product: LPCAT1 Antibody
Catalog: DF12033
Description: Rabbit polyclonal antibody to LPCAT1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Dog, Chicken
Mol.Wt.: 59 kDa; 59kD(Calculated).
Uniprot: Q8NF37
RRID: AB_2844838

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(91%), Bovine(92%), Horse(100%), Dog(92%), Chicken(90%)
Clonality:
Polyclonal
Specificity:
LPCAT1 Antibody detects endogenous levels of total LPCAT1.
RRID:
AB_2844838
Cite Format: Affinity Biosciences Cat# DF12033, RRID:AB_2844838.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1 acylglycerophosphocholine O acyltransferase; 1 alkylglycerophosphocholine O acetyltransferase; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; Acetyl CoA:lyso PAF acetyltransferase; Acetyl CoA:lyso platelet activating factor acetyltransferase; Acetyl-CoA:lyso-PAF acetyltransferase; Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Acyl CoA:lysophosphatidylcholine acyltransferase 1; Acyltransferase like 2; Acyltransferase-like 2; AGPAT10; AGPAT9; AYTL1; AYTL2; FLJ12443; FLJ41609; LPC acyltransferase 1; LPC acyltransferase; lpcat; LPCAT-1; LPCAT1; Lyso PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT; LysoPC acyltransferase 1; Lysophosphatidylcholine acyltransferase 1; PCAT1_HUMAN; PFAAP3; Phosphonoformate immuno associated protein 3; Phosphonoformate immuno-associated protein 3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MRLRGCGPRAAPASSAGASDARLLAPPGRNPFVHELRLSALQKAQVALMTLTLFPVRLLVAAAMMLLAWPLALVASLGSAEKEPEQPPALWRKVVDFLLKAIMRTMWFAGGFHRVAVKGRQALPTEAAILTLAPHSSYFDAIPVTMTMSSIVMKAESRDIPIWGTLIQYIRPVFVSRSDQDSRRKTVEEIKRRAQSNGKWPQIMIFPEGTCTNRTCLITFKPGAFIPGAPVQPVVLRYPNKLDTITWTWQGPGALEILWLTLCQFHNQVEIEFLPVYSPSEEEKRNPALYASNVRRVMAEALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGLKPEKLEKDLDRYSERARMKGGEKIGIAEFAASLEVPVSDLLEDMFSLFDESGSGEVDLRECVVALSVVCRPARTLDTIQLAFKMYGAQEDGSVGEGDLSCILKTALGVAELTVTDLFRAIDQEEKGKITFADFHRFAEMYPAFAEEYLYPDQTHFESCAETSPAPIPNGFCADFSPENSDAGRKPVRKKLD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
92
Dog
92
Zebrafish
91
Chicken
90
Pig
75
Xenopus
75
Sheep
67
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NF37 As Substrate

Site PTM Type Enzyme
T52 Phosphorylation
K93 Ubiquitination
S178 Phosphorylation
K191 Ubiquitination
K199 Ubiquitination
K221 Acetylation
K221 Ubiquitination
S292 Phosphorylation
K344 Ubiquitination
K362 Ubiquitination
K470 Ubiquitination

Research Backgrounds

Function:

Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-independent (By similarity). Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology. Involved in the regulation of lipid droplet number and size.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type II membrane protein. Golgi apparatus membrane>Single-pass type II membrane protein. Lipid droplet.
Note: May adopt a monotopic topology when embedded in the lipid monolayer of the lipid droplet, with both termini exposed to the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphocholine.

The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.

Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Research Fields

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Lipid metabolism > Ether lipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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