ECHS1 Antibody - #DF12025
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12025, RRID:AB_2844830.
Fold/Unfold
C80529; EC 4.2.1.17; ECHS 1; Enoyl CoA hydratase 1; Enoyl CoA hydratase mitochondrial; Enoyl CoA hydratase short chain 1 mitochondrial; Enoyl Coenzyme A hydratase short chain 1; Enoyl Coenzyme A hydratase short chain 1 mitochondrial; Mitochondrial short chain enoyl coenzyme A hydratase 1; OTTHUMP00000020811; SCEH; Short chain enoyl CoA hydratase;
Immunogens
- P30084 ECHM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAALRVLLSCVRGPLRPPVRCPAWRPFASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKTFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P30084 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y35 | Phosphorylation | Uniprot | |
| K40 | Acetylation | Uniprot | |
| T46 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| C62 | S-Nitrosylation | Uniprot | |
| K92 | Acetylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K118 | Acetylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| Y137 | Phosphorylation | Uniprot | |
| T176 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| T194 | Phosphorylation | Uniprot | |
| R197 | Methylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| K204 | Acetylation | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| C213 | S-Nitrosylation | Uniprot | |
| C225 | S-Nitrosylation | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| K261 | Ubiquitination | Uniprot |
Research Backgrounds
Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.
Mitochondrion matrix.
Liver, fibroblast, muscle. Barely detectable in spleen and kidney.
Homohexamer; dimer of trimers.
Belongs to the enoyl-CoA hydratase/isomerase family.
Research Fields
· Metabolism > Lipid metabolism > Fatty acid elongation.
· Metabolism > Lipid metabolism > Fatty acid degradation.
· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.
· Metabolism > Amino acid metabolism > Lysine degradation.
· Metabolism > Amino acid metabolism > Tryptophan metabolism.
· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.
· Metabolism > Carbohydrate metabolism > Propanoate metabolism.
· Metabolism > Carbohydrate metabolism > Butanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Fatty acid metabolism.
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