Product: TPI1 Antibody
Catalog: DF12023
Description: Rabbit polyclonal antibody to TPI1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 27kd; 31kD(Calculated).
Uniprot: P60174
RRID: AB_2844828

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
TPI1 Antibody detects endogenous levels of total TPI1.
RRID:
AB_2844828
Cite Format: Affinity Biosciences Cat# DF12023, RRID:AB_2844828.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

epididymis secretory protein Li 49; HEL-S-49; MGC88108; TIM; TPI 1; TPI; TPI1; TPID; Triose phosphate isomerase 1; Triose phosphate isomerase; Triosephosphate isomerase 1; Triosephosphate isomerase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAEDGEEAEFHFAALYISGQWPRLRADTDLQRLGSSAMAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
82
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P60174 As Substrate

Site PTM Type Enzyme
Y16 Phosphorylation
T28 Phosphorylation
K43 Acetylation
K43 Ubiquitination
K51 Acetylation
K51 Methylation
K51 Ubiquitination
K56 Ubiquitination
S58 Phosphorylation
T65 Phosphorylation
K70 Ubiquitination
Y85 Phosphorylation
K92 Ubiquitination
K96 Acetylation
K96 Ubiquitination
Y105 Phosphorylation
K106 Acetylation
K106 Methylation
K106 Ubiquitination
T108 Phosphorylation
S117 Phosphorylation
K122 Ubiquitination
T127 Phosphorylation
R137 Methylation
S143 Phosphorylation
K168 Acetylation
K168 Ubiquitination
R172 Methylation
T177 Phosphorylation
K179 Acetylation
K179 Sumoylation
K179 Ubiquitination
K186 Acetylation
K186 Ubiquitination
K193 Acetylation
K193 Ubiquitination
S196 Phosphorylation
K197 Ubiquitination
Y202 Phosphorylation
T210 Phosphorylation
K212 Acetylation
K212 Ubiquitination
T215 Phosphorylation
K225 Acetylation
K225 Ubiquitination
K231 Acetylation
K231 Methylation
K231 Ubiquitination
S232 Phosphorylation
S235 Phosphorylation
S241 Phosphorylation
Y246 Phosphorylation
S249 Phosphorylation
T251 Phosphorylation
T254 Phosphorylation
C255 S-Nitrosylation
K256 Ubiquitination
S260 Phosphorylation
K275 Acetylation
K275 Ubiquitination
K285 Acetylation
K285 Ubiquitination

Research Backgrounds

Function:

Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.

It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.

PTMs:

The initiator methionine for isoform 2 is removed.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the triosephosphate isomerase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

References

1). A prospective diagnostic and prognostic biomarker for hepatocellular carcinoma that functions in glucose metabolism regulation: Solute carrier family 37 member 3. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2023 (PubMed: 36773462) [IF=6.2]

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