RB1CC1 Antibody - #DF12022

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Product: RB1CC1 Antibody
Catalog: DF12022
Description: Rabbit polyclonal antibody to RB1CC1
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 200 kDa; 183kD(Calculated).
Uniprot: Q8TDY2
RRID: AB_2844827

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 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Bovine(92%), Horse(100%), Sheep(92%), Rabbit(80%), Dog(100%)
Clonality:
Polyclonal
Specificity:
RB1CC1 Antibody detects endogenous levels of total RB1CC1.
RRID:
AB_2844827
Cite Format: Affinity Biosciences Cat# DF12022, RRID:AB_2844827.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FAK family kinase-interacting protein of 200 kDa; FIP200; RB1-inducible coiled-coil protein 1; RB1CC1; RBCC1_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q8TDY2(RBCC1_HUMAN) >>Visit HPA database.

Gene(ID):
RB1CC1(9821)
Expression:
Q8TDY2 RBCC1_HUMAN:

Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells.

Sequence:
MKLYVFLVNTGTTLTFDTELTVQTVADLKHAIQSKYKIAIQHQVLVVNGGECMAADRRVCTYSAGTDTNPIFLFNKEMILCDRPPAIPKTTFSTENDMEIKVEESLMMPAVFHTVASRTQLALEMYEVAKKLCSFCEGLVHDEHLQHQGWAAIMANLEDCSNSYQKLLFKFESIYSNYLQSIEDIKLKLTHLGTAVSVMAKIPLLECLTRHSYRECLGRLDSLPEHEDSEKAEMKRSTELVLSPDMPRTTNESLLTSFPKSVEHVSPDTADAESGKEIRESCQSTVHQQDETTIDTKDGDLPFFNVSLLDWINVQDRPNDVESLVRKCFDSMSRLDPRIIRPFIAECRQTIAKLDNQNMKAIKGLEDRLYALDQMIASCGRLVNEQKELAQGFLANQKRAENLKDASVLPDLCLSHANQLMIMLQNHRKLLDIKQKCTTAKQELANNLHVRLKWCCFVMLHADQDGEKLQALLRLVIELLERVKIVEALSTVPQMYCLAVVEVVRRKMFIKHYREWAGALVKDGKRLYEAEKSKRESFGKLFRKSFLRNRLFRGLDSWPPSFCTQKPRKFDCELPDISLKDLQFLQSFCPSEVQPFLRVPLLCDFEPLHQHVLALHNLVKAAQSLDEMSQTITDLLSEQKASVSQTSPQSASSPRMESTAGITTTTSPRTPPPLTVQDPLCPAVCPLEELSPDSIDAHTFDFETIPHPNIEQTIHQVSLDLDSLAESPESDFMSAVNEFVIEENLSSPNPISDPQSPEMMVESLYSSVINAIDSRRMQDTNVCGKEDFGDHTSLNVQLERCRVVAQDSHFSIQTIKEDLCHFRTFVQKEQCDFSNSLKCTAVEIRNIIEKVKCSLEITLKEKHQKELLSLKNEYEGKLDGLIKETEENENKIKKLKGELVCLEEVLQNKDNEFALVKHEKEAVICLQNEKDQKLLEMENIMHSQNCEIKELKQSREIVLEDLKKLHVENDEKLQLLRAELQSLEQSHLKELEDTLQVRHIQEFEKVMTDHRVSLEELKKENQQIINQIQESHAEIIQEKEKQLQELKLKVSDLSDTRCKLEVELALKEAETDEIKILLEESRAQQKETLKSLLEQETENLRTEISKLNQKIQDNNENYQVGLAELRTLMTIEKDQCISELISRHEEESNILKAELNKVTSLHNQAFEIEKNLKEQIIELQSKLDSELSALERQKDEKITQQEEKYEAIIQNLEKDRQKLVSSQEQDREQLIQKLNCEKDEAIQTALKEFKLEREVVEKELLEKVKHLENQIAKSPAIDSTRGDSSSLVAELQEKLQEEKAKFLEQLEEQEKRKNEEMQNVRTSLIAEQQTNFNTVLTREKMRKENIINDLSDKLKSTMQQQERDKDLIESLSEDRARLLEEKKKLEEEVSKLRSSSFVPSPYVATAPELYGACAPELPGESDRSAVETADEGRVDSAMETSMMSVQENIHMLSEEKQRIMLLERTLQLKEEENKRLNQRLMSQSMSSVSSRHSEKIAIRDFQVGDLVLIILDERHDNYVLFTVSPTLYFLHSESLPALDLKPGEGASGASRRPWVLGKVMEKEYCQAKKAQNRFKVPLGTKFYRVKAVSWNKKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Bovine
92
Sheep
92
Rabbit
80
Xenopus
70
Zebrafish
70
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TDY2 As Substrate

Site PTM Type Enzyme
Phosphorylation
K35 Ubiquitination
S105 Phosphorylation
S134 Phosphorylation
T209 Phosphorylation
R210 Methylation
S212 Phosphorylation
R214 Methylation
S222 Phosphorylation
S229 Phosphorylation
S237 Phosphorylation
T238 Phosphorylation
S243 Phosphorylation
S253 Phosphorylation
T256 Phosphorylation
S257 Phosphorylation
K260 Ubiquitination
S261 Phosphorylation
S266 Phosphorylation
S274 Phosphorylation
K387 Ubiquitination
K434 Ubiquitination
S490 Phosphorylation
T491 Phosphorylation
Y496 Phosphorylation
K522 Ubiquitination
S537 Phosphorylation
S624 Phosphorylation
S629 Phosphorylation
T633 Phosphorylation
S642 Phosphorylation
S644 Phosphorylation
T646 Phosphorylation
S647 Phosphorylation
S650 Phosphorylation
S652 Phosphorylation
S653 Phosphorylation
T664 Phosphorylation
T670 Phosphorylation
S793 Phosphorylation
K828 Ubiquitination
K865 Ubiquitination
K871 Ubiquitination
K877 Ubiquitination
K883 Acetylation
K891 Acetylation
K896 Ubiquitination
K917 Ubiquitination
S943 Phosphorylation
K963 Sumoylation
K963 Ubiquitination
S982 Phosphorylation
S986 Phosphorylation
K989 Ubiquitination
T994 Phosphorylation
S1051 Phosphorylation
K1090 Ubiquitination
K1110 Ubiquitination
S1160 Phosphorylation
K1204 Ubiquitination
Y1205 Phosphorylation
S1222 Phosphorylation
K1233 Ubiquitination
K1247 Ubiquitination
K1263 Acetylation
S1274 Phosphorylation
S1323 Phosphorylation
S1370 Phosphorylation
T1440 Phosphorylation
S1441 Phosphorylation
S1482 Phosphorylation
S1484 Phosphorylation
K1581 Ubiquitination

Research Backgrounds

Function:

Involved in autophagy. Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1. Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S.typhimurium infection and subsequent xenophagy (By similarity). Involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways. Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111 (By similarity). Functions as a DNA-binding transcription factor. Is a potent regulator of the RB1 pathway through induction of RB1 expression. Plays a crucial role in muscular differentiation. Plays an indispensable role in fetal hematopoiesis and in the regulation of neuronal homeostasis (By similarity).

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm>Cytosol. Preautophagosomal structure. Lysosome.
Note: Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells.

Subunit Structure:

Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. This complex associates with ATG101. Interacts with PTK2/FAK1 and PTK2B/PYK2. Interacts with GABARAP and GABARAPL1. Interacts with ATG16L1; the interaction is required for ULK1 complex-dependent autophagy. Interacts with RNF111, SKI and SMAD7 (By similarity). Interacts with COP1 in the cytoplasm of proliferating cells in response to UV stimulation. Interacts with TP53. Interacts with C9orf72. Interacts with WDR45B.

Family&Domains:

Belongs to the ATG17 family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

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