SHARPIN Antibody - #DF12007
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12007, RRID:AB_2844812.
Fold/Unfold
DKFZp434N1923; hSIPL1; Shank associated RH domain interacting protein; SHANK associated RH domain interactor; Shank interacting protein like 1; Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1; Sharpin; SHRPN_HUMAN; SIPL1;
Immunogens
Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Up-regulated in various tumor tissues such as kidney, liver, ovary and pancreas tumors.
- Q9H0F6 SHRPN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPPAGGAAAAASDLGSAAVLLAVHAAVRPLGAGPDAEAQLRRLQLSADPERPGRFRLELLGAGPGAVNLEWPLESVSYTIRGPTQHELQPPPGGPGTLSLHFLNPQEAQRWAVLVRGATVEGQNGSKSNSPPALGPEACPVSLPSPPEASTLKGPPPEADLPRSPGNLTEREELAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASAASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDPAFLYLLSAPREAPATGPSPQHPQKMDGELGRLFPPSLGLPPGPQPAASSLPSPLQPSWSCPSCTFINAPDRPGCEMCSTQRPCTWDPLAAAST
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H0F6 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R29 | Methylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| T152 | Phosphorylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| K318 | Ubiquitination | Uniprot |
Research Backgrounds
Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.
Cytoplasm>Cytosol. Cell junction>Synapse.
Note: Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Up-regulated in various tumor tissues such as kidney, liver, ovary and pancreas tumors.
Monomer and homodimer (By similarity). Component of the LUBAC complex (linear ubiquitin chain assembly complex) which consists of SHARPIN, RBCK1 and RNF31. LUBAC has a MW of approximately 600 kDa suggesting a heteromultimeric assembly of its subunits. Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation-dependent manner. Interacts with EYA1, EYA2, SHANK1 and SHANK3 (via ANK repeats) (By similarity).
The Ubiquitin-like domain is required for the interaction with RNF31.
The RanBP2-type zinc fingers mediate the specific interaction with ubiquitin. Binds preferentially linear polyubiquitin chains and 'Lys-63'-linked polyubiquitin chains over 'Lys-48'-linked polyubiquitin chains. Also binds monoubiquitin.
Research Fields
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
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