Scribble Antibody | Affinity Biosciences

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Western blot analysis of Scribble in lysates of HCT 116, using Scribble Antibody(AF4771).

Product:	Scribble Antibody
Catalog:	AF4771
Description:	Rabbit polyclonal antibody to Scribble
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse
Mol.Wt.:	240kDa; 175kD(Calculated).
Uniprot:	Q14160
RRID:	AB_2844763

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(83%), Bovine(83%), Horse(92%)

Clonality:

Polyclonal

Specificity:

Scribble Antibody detects endogenous levels of total Scribble.

RRID:

AB_2844763
Cite Format: Affinity Biosciences Cat# AF4771, RRID:AB_2844763.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CRIB 1; CRIB1; hScrib; LAP 4; LAP4; PDZ domain protein scribble; Protein LAP4; Protein scribble homolog; SCRB 1; SCRB1; SCRIB 1; Scrib; SCRIB_HUMAN; SCRIB1; Scribble; Scribble homolog 1; scribbled homolog (Drosophila); Vartul;

Immunogens

Immunogen:

Uniprot:

Q14160(SCRIB_HUMAN) >>Visit HPA database.

Gene(ID):

SCRIB(23513)

Expression:

Q14160 SCRIB_HUMAN:

Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).

Sequence:

Q14160 SCRIB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLKCIPLWRCNRHVESVDKRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRLPDGFTQLRSLAHLALNDVSLQALPGDVGNLANLVTLELRENLLKSLPASLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENRLEELPAELGGLVLLTDLLLSQNLLRRLPDGIGQLKQLSILKVDQNRLCEVTEAIGDCENLSELILTENLLMALPRSLGKLTKLTNLNVDRNHLEALPPEIGGCVALSVLSLRDNRLAVLPPELAHTTELHVLDVAGNRLQSLPFALTHLNLKALWLAENQAQPMLRFQTEDDARTGEKVLTCYLLPQQPPPSLEDAGQQGSLSETWSDAPPSRVSVIQFLEAPIGDEDAEEAAAEKRGLQRRATPHPSELKVMKRSIEGRRSEACPCQPDSGSPLPAEEEKRLSAESGLSEDSRPSASTVSEAEPEGPSAEAQGGSQQEATTAGGEEDAEEDYQEPTVHFAEDALLPGDDREIEEGQPEAPWTLPGGRQRLIRKDTPHYKKHFKISKLPQPEAVVALLQGMQPDGEGPVAPGGWHNGPHAPWAPRAQKEEEEEEEGSPQEEEVEEEEENRAEEEEASTEEEDKEGAVVSAPSVKGVSFDQANNLLIEPARIEEEELTLTILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVALQGAEHHEAVEALRGAGTAVQMRVWRERMVEPENAVTITPLRPEDDYSPRERRGGGLRLPLLPPESPGPLRQRHVACLARSERGLGFSIAGGKGSTPYRAGDAGIFVSRIAEGGAAHRAGTLQVGDRVLSINGVDVTEARHDHAVSLLTAASPTIALLLEREAGGPLPPSPLPHSSPPTAAVATTSITTATPGVPGLPSLAPSLLAAALEGPYPVEEIRLPRAGGPLGLSIVGGSDHSSHPFGVQEPGVFISKVLPRGLAARSGLRVGDRILAVNGQDVRDATHQEAVSALLRPCLELSLLVRRDPAPPGLRELCIQKAPGERLGISIRGGARGHAGNPRDPTDEGIFISKVSPTGAAGRDGRLRVGLRLLEVNQQSLLGLTHGEAVQLLRSVGDTLTVLVCDGFEASTDAALEVSPGVIANPFAAGIGHRNSLESISSIDRELSPEGPGKEKELPGQTLHWGPEATEAAGRGLQPLKLDYRALAAVPSAGSVQRVPSGAAGGKMAESPCSPSGQQPPSPPSPDELPANVKQAYRAFAAVPTSHPPEDAPAQPPTPGPAASPEQLSFRERQKYFELEVRVPQAEGPPKRVSLVGADDLRKMQEEEARKLQQKRAQMLREAAEAGAEARLALDGETLGEEEQEDEQPPWASPSPTSRQSPASPPPLGGGAPVRTAKAERRHQERLRVQSPEPPAPERALSPAELRALEAEKRALWRAARMKSLEQDALRAQMVLSRSQEGRGTRGPLERLAEAPSPAPTPSPTPVEDLGPQTSTSPGRLSPDFAEELRSLEPSPSPGPQEEDGEVALVLLGRPSPGAVGPEDVALCSSRRPVRPGRRGLGPVPS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Pig

Bovine

Dog

Zebrafish

Sheep

Xenopus

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14160 As Substrate

Q14160

Site	PTM Type	Enzyme	Source
K19	Ubiquitination		Uniprot
C22	S-Nitrosylation		Uniprot
S23	Phosphorylation		Uniprot
Y32	Phosphorylation		Uniprot
Y34	Phosphorylation		Uniprot
S35	Phosphorylation		Uniprot
S37	Phosphorylation		Uniprot
K53	Ubiquitination		Uniprot
K63	Ubiquitination		Uniprot
S67	Phosphorylation		Uniprot
K102	Ubiquitination		Uniprot
K105	Ubiquitination		Uniprot
K164	Ubiquitination		Uniprot
S165	Phosphorylation		Uniprot
K266	Ubiquitination		Uniprot
K272	Ubiquitination		Uniprot
K313	Ubiquitination		Uniprot
T378	Phosphorylation		Uniprot
S432	Phosphorylation		Uniprot
S446	Phosphorylation		Uniprot
K467	Ubiquitination		Uniprot
T475	Phosphorylation		Uniprot
S487	Phosphorylation		Uniprot
S493	Phosphorylation		Uniprot
S504	Phosphorylation		Uniprot
K512	Ubiquitination		Uniprot
S515	Phosphorylation		Uniprot
T553	Phosphorylation	P27361 (MAPK3)	Uniprot
Y564	Phosphorylation		Uniprot
T607	Phosphorylation		Uniprot
Y610	Phosphorylation		Uniprot
S668	Phosphorylation		Uniprot
S688	Phosphorylation		Uniprot
T689	Phosphorylation		Uniprot
S703	Phosphorylation		Uniprot
K705	Ubiquitination		Uniprot
S708	Phosphorylation		Uniprot
T728	Phosphorylation		Uniprot
T730	Phosphorylation		Uniprot
T735	Phosphorylation		Uniprot
S741	Phosphorylation		Uniprot
S748	Phosphorylation		Uniprot
T749	Phosphorylation		Uniprot
K752	Ubiquitination		Uniprot
S764	Phosphorylation		Uniprot
T824	Phosphorylation		Uniprot
T826	Phosphorylation		Uniprot
Y834	Phosphorylation		Uniprot
S835	Phosphorylation		Uniprot
S853	Phosphorylation		Uniprot
S868	Phosphorylation		Uniprot
S875	Phosphorylation		Uniprot
K880	Ubiquitination		Uniprot
T883	Phosphorylation		Uniprot
R886	Methylation		Uniprot
S917	Phosphorylation		Uniprot
S939	Phosphorylation		Uniprot
T941	Phosphorylation		Uniprot
Y1000	Phosphorylation		Uniprot
S1017	Phosphorylation		Uniprot
K1040	Ubiquitination		Uniprot
S1050	Phosphorylation		Uniprot
K1105	Ubiquitination		Uniprot
S1114	Phosphorylation		Uniprot
R1116	Methylation		Uniprot
R1120	Methylation		Uniprot
K1138	Ubiquitination		Uniprot
S1140	Phosphorylation		Uniprot
S1220	Phosphorylation		Uniprot
S1223	Phosphorylation		Uniprot
S1225	Phosphorylation		Uniprot
S1226	Phosphorylation		Uniprot
S1232	Phosphorylation		Uniprot
K1240	Ubiquitination		Uniprot
R1259	Methylation		Uniprot
K1265	Ubiquitination		Uniprot
Y1268	Phosphorylation		Uniprot
S1276	Phosphorylation		Uniprot
S1279	Phosphorylation		Uniprot
S1285	Phosphorylation		Uniprot
S1295	Phosphorylation		Uniprot
S1298	Phosphorylation		Uniprot
S1300	Phosphorylation		Uniprot
S1306	Phosphorylation		Uniprot
S1309	Phosphorylation		Uniprot
T1342	Phosphorylation		Uniprot
S1348	Phosphorylation		Uniprot
S1353	Phosphorylation		Uniprot
Y1360	Phosphorylation		Uniprot
S1378	Phosphorylation	Q13464 (ROCK1)	Uniprot
S1437	Phosphorylation		Uniprot
S1439	Phosphorylation		Uniprot
S1442	Phosphorylation		Uniprot
S1445	Phosphorylation		Uniprot
S1448	Phosphorylation	P27361 (MAPK3)	Uniprot
T1460	Phosphorylation		Uniprot
S1475	Phosphorylation		Uniprot
S1486	Phosphorylation		Uniprot
K1507	Methylation		Uniprot
S1508	Phosphorylation	Q13464 (ROCK1)	Uniprot
S1521	Phosphorylation		Uniprot
S1523	Phosphorylation		Uniprot
R1527	Methylation		Uniprot
T1529	Phosphorylation		Uniprot
S1541	Phosphorylation		Uniprot
T1545	Phosphorylation		Uniprot
S1547	Phosphorylation		Uniprot
T1549	Phosphorylation		Uniprot
T1558	Phosphorylation		Uniprot
S1559	Phosphorylation		Uniprot
T1560	Phosphorylation		Uniprot
S1561	Phosphorylation		Uniprot
S1566	Phosphorylation		Uniprot
S1579	Phosphorylation		Uniprot
S1581	Phosphorylation		Uniprot
S1600	Phosphorylation		Uniprot
S1613	Phosphorylation		Uniprot
S1630	Phosphorylation		Uniprot

Research Backgrounds

Q14160_SCRIB_HUMAN

Function:

Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.

PTMs:

Ubiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded.

Subcellular Location:

Cell membrane>Peripheral membrane protein. Cell junction>Adherens junction. Cell projection>Lamellipodium. Cytoplasm. Cell junction>Synapse>Postsynapse. Cell junction>Synapse>Presynapse.
Note: Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity.

Tissue Specificity:

Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).

Subunit Structure:

Interacts (via PDZ domains) with VANGL2. Interacts with CTNNB1 and MAPK12 (By similarity). Interacts with UBE3A and HPV E6. Interacts with PAK1 and PAK2. Interacts with ARHGEF7 and GIT1; interacts directly with ARHGEF7. Interacts (via PDZ domains) with LPP and TRIP6; the interaction is direct. Interacts (via PDZ domains) with TJP2. Interacts (via PDZ domains) with APC; may mediate APC targeting to adherens junctions of epithelial cells. Interacts (via PDZ domains) with TSHR; regulates TSHR trafficking and function. Interacts (via PDZ domains 1 and 3) with MCC. Interacts (via fourth PDZ domain) with tick-borne encephalitis virus NS5 protein; this interaction inhibits SCRIB and downstream STAT1 phosphorylation, thereby preventing activation of JAK-STAT signaling pathway. Interacts with DLG5. Interacts with STK4/MST1 and LATS1 in the presence of DLG5.

(Microbial infection) Interacts with tick-borne encephalitis virus RNA-directed RNA polymerase NS5; this interaction targets viral NS5 to the cell membrane periphery and nucleus, thereby allowing efficient host nuclear STAT1 inhibition.

Family&Domains:

Belongs to the LAP (LRR and PDZ) protein family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

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Scribble Antibody - #AF4771