PLD1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from B16F10 cells, using PLD1 Antibody.

Product:	PLD1 Antibody
Catalog:	AF4765
Description:	Rabbit polyclonal antibody to PLD1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	120kDa; 124kD(Calculated).
Uniprot:	Q13393
RRID:	AB_2844759

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Dog(92%)

Clonality:

Polyclonal

Specificity:

PLD1 Antibody detects endogenous levels of total PLD1.

RRID:

AB_2844759
Cite Format: Affinity Biosciences Cat# AF4765, RRID:AB_2844759.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Choline phosphatase 1; hPLD1; Phosphatidylcholine-hydrolyzing phospholipase D1; Phospholipase D1; PLD 1; PLD1; PLD1_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q13393(PLD1_HUMAN) >>Visit HPA database.

Gene(ID):

PLD1(5337)

Expression:

Q13393 PLD1_HUMAN:

Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.

Sequence:

Q13393 PLD1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSLKNEPRVNTSALQKIAADMSNIIENLDTRELHFEGEEVDYDVSPSDPKIQEVYIPFSAIYNTQGFKEPNIQTYLSGCPIKAQVLEVERFTSTTRVPSINLYTIELTHGEFKWQVKRKFKHFQEFHRELLKYKAFIRIPIPTRRHTFRRQNVREEPREMPSLPRSSENMIREEQFLGRRKQLEDYLTKILKMPMYRNYHATTEFLDISQLSFIHDLGPKGIEGMIMKRSGGHRIPGLNCCGQGRACYRWSKRWLIVKDSFLLYMKPDSGAIAFVLLVDKEFKIKVGKKETETKYGIRIDNLSRTLILKCNSYRHARWWGGAIEEFIQKHGTNFLKDHRFGSYAAIQENALAKWYVNAKGYFEDVANAMEEANEEIFITDWWLSPEIFLKRPVVEGNRWRLDCILKRKAQQGVRIFIMLYKEVELALGINSEYTKRTLMRLHPNIKVMRHPDHVSSTVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDNEHRLTDVGSVKRVTSGPSLGSLPPAAMESMESLRLKDKNEPVQNLPIQKSIDDVDSKLKGIGKPRKFSKFSLYKQLHRHHLHDADSISSIDSTSSYFNHYRSHHNLIHGLKPHFKLFHPSSESEQGLTRPHADTGSIRSLQTGVGELHGETRFWHGKDYCNFVFKDWVQLDKPFADFIDRYSTPRMPWHDIASAVHGKAARDVARHFIQRWNFTKIMKSKYRSLSYPFLLPKSQTTAHELRYQVPGSVHANVQLLRSAADWSAGIKYHEESIHAAYVHVIENSRHYIYIENQFFISCADDKVVFNKIGDAIAQRILKAHRENQKYRVYVVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGENSILGQLKAELGNQWINYISFCGLRTHAELEGNLVTELIYVHSKLLIADDNTVIIGSANINDRSMLGKRDSEMAVIVQDTETVPSVMDGKEYQAGRFARGLRLQCFRVVLGYLDDPSEDIQDPVSDKFFKEVWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPVLAKEDPIRAEEELKKIRGFLVQFPFYFLSEESLLPSVGTKEAIVPMEVWT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13393 As Substrate

Q13393

Site	PTM Type	Enzyme	Source
S2	Phosphorylation	P17252 (PRKCA)	Uniprot
S12	Phosphorylation		Uniprot
K16	Ubiquitination		Uniprot
S22	Phosphorylation		Uniprot
Y42	Phosphorylation		Uniprot
S45	Phosphorylation		Uniprot
K50	Ubiquitination		Uniprot
K121	Ubiquitination		Uniprot
K132	Methylation		Uniprot
T147	Phosphorylation	P17252 (PRKCA)	Uniprot
S162	Phosphorylation		Uniprot
K189	Ubiquitination		Uniprot
K294	Ubiquitination		Uniprot
K406	Methylation		Uniprot
S431	Phosphorylation		Uniprot
T495	Phosphorylation		Uniprot
S499	Phosphorylation		Uniprot
K501	Ubiquitination		Uniprot
S505	Phosphorylation	P54646 (PRKAA2)	Uniprot
S508	Phosphorylation		Uniprot
S519	Phosphorylation		Uniprot
S522	Phosphorylation		Uniprot
S540	Phosphorylation		Uniprot
K547	Ubiquitination		Uniprot
S558	Phosphorylation		Uniprot
K559	Ubiquitination		Uniprot
S561	Phosphorylation	P17252 (PRKCA)	Uniprot
Y586	Phosphorylation		Uniprot
Y590	Phosphorylation		Uniprot
S610	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S611	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S629	Phosphorylation		Uniprot
K662	Ubiquitination		Uniprot
K688	Ubiquitination		Uniprot
Y711	Phosphorylation		Uniprot
K796	Ubiquitination		Uniprot
K807	Acetylation		Uniprot
K1000	Ubiquitination		Uniprot
K1027	Ubiquitination		Uniprot

Research Backgrounds

Q13393_PLD1_HUMAN

Function:

Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).

Subcellular Location:

Cytoplasm>Perinuclear region. Endoplasmic reticulum membrane>Lipid-anchor>Cytoplasmic side. Golgi apparatus membrane>Lipid-anchor>Cytoplasmic side. Late endosome membrane>Lipid-anchor>Cytoplasmic side.

Tissue Specificity:

Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.

Subunit Structure:

Interacts with PIP5K1B.

Family&Domains:

Belongs to the phospholipase D family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Lipid metabolism > Ether lipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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PLD1 Antibody - #AF4765