Phospho-Ephrin B2 (Tyr330) Antibody

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Product:	Phospho-Ephrin B2 (Tyr330) Antibody
Catalog:	AF3343
Description:	Rabbit polyclonal antibody to Phospho-Ephrin B2 (Tyr330)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	37kDa; 37kD(Calculated).
Uniprot:	P52799
RRID:	AB_2834758

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-Ephrin B2 (Tyr330) Antibody detects endogenous levels of Ephrin B2 only when phosphorylated at Tyrosine 330.

RRID:

AB_2834758
Cite Format: Affinity Biosciences Cat# AF3343, RRID:AB_2834758.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EFN B2; EFNB 2; Efnb2; EFNB2_HUMAN; Eph related receptor tyrosine kinase ligand 5; EPH-related receptor tyrosine kinase ligand 5; ephrin B2; Ephrin-B2; EphrinB2; EPLG 5; EPLG5; Htk L; HTK ligand; HTK-L; HTKL; LERK 5; LERK-5; LERK5; Ligand of eph related kinase 5; MGC126226; MGC126227; MGC126228; OTTMUSP00000024973;

Immunogens

Immunogen:

Uniprot:

P52799(EFNB2_HUMAN) >>Visit HPA database.

Gene(ID):

EFNB2(1948)

Expression:

P52799 EFNB2_HUMAN:

Lung and kidney.

Description:

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis.

Sequence:

P52799 EFNB2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAVRRDSVWKYCWGVLMVLCRTAISKSIVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADRCTIKKENTPLLNCAKPDQDIKFTIKFQEFSPNLWGLEFQKNKDYYIISTSNGSLEGLDNQEGGVCQTRAMKILMKVGQDASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILGSEVALFAGIASGCIIFIVIIITLVVLLLKYRRRHRKHSPQHTTTLSLSTLATPKRSGNNNGSEPSDIIIPLRTADSVFCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52799 As Substrate

P52799

Site	PTM Type	Enzyme	Source
Y11	Phosphorylation		Uniprot
N36	N-Glycosylation		Uniprot
T69	Phosphorylation		Uniprot
T180	O-Glycosylation		Uniprot
T189	O-Glycosylation		Uniprot
S260	Phosphorylation		Uniprot
T264	Phosphorylation		Uniprot
S268	Phosphorylation		Uniprot
S270	Phosphorylation		Uniprot
T274	Phosphorylation		Uniprot
Y304	Phosphorylation	P29323 (EPHB2)	Uniprot
S308	Phosphorylation		Uniprot
Y311	Phosphorylation		Uniprot
Y316	Phosphorylation		Uniprot
S325	Phosphorylation		Uniprot
Y330	Phosphorylation		Uniprot
Y331	Phosphorylation		Uniprot
K332	Ubiquitination		Uniprot

Research Backgrounds

P52799_EFNB2_HUMAN

Function:

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.

(Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.

PTMs:

Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell junction>Adherens junction.

Tissue Specificity:

Lung and kidney.

Subunit Structure:

Interacts with PDZRN3 (By similarity). Binds to the receptor tyrosine kinases EPHA4, EPHB4 and EPHA3.

(Microbial infection) Interacts with Hendra virus and Nipah virus G protein.

Family&Domains:

Belongs to the ephrin family.

Research Fields

· Organismal Systems > Development > Axon guidance. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-Ephrin B2 (Tyr330) Antibody - #AF3343