MUNC-18a Antibody - #AF4751

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Product: MUNC-18a Antibody
Catalog: AF4751
Description: Rabbit polyclonal antibody to MUNC-18a
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 70kDa; 68kD(Calculated).
Uniprot: P61764
RRID: AB_2844746

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
MUNC-18a Antibody detects endogenous levels of total MUNC-18a.
RRID:
AB_2844746
Cite Format: Affinity Biosciences Cat# AF4751, RRID:AB_2844746.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ37475; Munc 18 1; Munc 18a; MUNC18 1; N-Sec1; Neuronal SEC1; NSec1; p67; Protein unc-18 homolog 1; Protein unc-18 homolog A; Rb sec1; RBSEC1; STXB1_HUMAN; STXBP1; Syntaxin binding protein 1; Syntaxin-binding protein 1; Unc 18 homolog; Unc 18A; Unc-18A; Unc18 1; UNC18; Unc18-1;

Immunogens

Immunogen:
Uniprot:

P61764(STXB1_HUMAN) >>Visit HPA database.

Gene(ID):
STXBP1(6812)
Expression:
P61764 STXB1_HUMAN:

Brain and spinal cord. Highly enriched in axons.

Sequence:
MAPIGLKAVVGEKIMHDVIKKVKKKGEWKVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPLPSLEAVYLITPSEKSVHSLISDFKDPPTAKYRAAHVFFTDSCPDALFNELVKSRAAKVIKTLTEINIAFLPYESQVYSLDSADSFQSFYSPHKAQMKNPILERLAEQIATLCATLKEYPAVRYRGEYKDNALLAQLIQDKLDAYKADDPTMGEGPDKARSQLLILDRGFDPSSPVLHELTFQAMSYDLLPIENDVYKYETSGIGEARVKEVLLDEDDDLWIALRHKHIAEVSQEVTRSLKDFSSSKRMNTGEKTTMRDLSQMLKKMPQYQKELSKYSTHLHLAEDCMKHYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRAIVPILLDANVSTYDKIRIILLYIFLKNGITEENLNKLIQHAQIPPEDSEIITNMAHLGVPIVTDSTLRRRSKPERKERISEQTYQLSRWTPIIKDIMEDTIEDKLDTKHYPYISTRSSASFSTTAVSARYGHWHKNKAPGEYRSGPRLIIFILGGVSLNEMRCAYEVTQANGKWEVLIGSTHILTPQKLLDTLKKLNKTDEEISS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Chicken
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61764 As Substrate

Site PTM Type Enzyme
K7 Ubiquitination
K13 Ubiquitination
S70 Phosphorylation
K92 Ubiquitination
Y145 Phosphorylation
K184 Ubiquitination
K196 Ubiquitination
K208 Ubiquitination
K294 Ubiquitination
S306 Phosphorylation
K308 Ubiquitination
S313 Phosphorylation
K314 Ubiquitination
K321 Methylation
K321 Ubiquitination
K332 Ubiquitination
K333 Ubiquitination
Y337 Phosphorylation
K339 Ubiquitination
K364 Ubiquitination
T376 Phosphorylation
K382 Ubiquitination
S454 Phosphorylation
T455 Phosphorylation
Y473 Phosphorylation
T479 Phosphorylation Q13627 (DYRK1A)
T489 Phosphorylation
S507 Phosphorylation
S509 Phosphorylation
S516 Phosphorylation
Y531 Phosphorylation
T574 Phosphorylation
K587 Ubiquitination
S593 Phosphorylation
S594 Phosphorylation

Research Backgrounds

Function:

Participates in the regulation of synaptic vesicle docking and fusion through interaction with GTP-binding proteins (By similarity). Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.

Subcellular Location:

Cytoplasm>Cytosol. Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain and spinal cord. Highly enriched in axons.

Subunit Structure:

Interacts with SYTL4 (By similarity). Interacts with STX1A. Interacts with alpha-synuclein/SNCA; this interaction controls SNCA self-replicating aggregation. Interacts with RAB3A; this interaction promotes RAB3A dissociation from the vesicle membrane (By similarity). Interacts with CABP5 (By similarity).

Family&Domains:

Belongs to the STXBP/unc-18/SEC1 family.

Research Fields

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

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