RAD52 Antibody - #AF4731

(3)  
Product: RAD52 Antibody
Catalog: AF4731
Description: Rabbit polyclonal antibody to RAD52
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 46kDa; 46kD(Calculated).
Uniprot: P43351
RRID: AB_2844730

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
RAD52 Antibody detects endogenous levels of total RAD52.
RRID:
AB_2844730
Cite Format: Affinity Biosciences Cat# AF4731, RRID:AB_2844730.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA repair protein RAD52; DNA repair protein RAD52; DNA repair protein RAD52 homolog; RAD 52; Rad52; RAD52 homolog (S. cerevisiae); RAD52 homolog (S. cerevisiae); RAD52 homolog; RAD52_HUMAN; Recombination protein RAD52; Recombination protein RAD52; Rhabdomyosarcoma antigen MU RMS 40.23;

Immunogens

Immunogen:
Uniprot:

P43351(RAD52_HUMAN) >>Visit HPA database.

Gene(ID):
RAD52(5893)
Sequence:
MSGTEEAILGGRDSHPAAGGGSVLCFGQCQYTAEEYQAIQKALRQRLGPEYISSRMAGGGQKVCYIEGHRVINLANEMFGYNGWAHSITQQNVDFVDLNNGKFYVGVCAFVRVQLKDGSYHEDVGYGVSEGLKSKALSLEKARKEAVTDGLKRALRSFGNALGNCILDKDYLRSLNKLPRQLPLEVDLTKAKRQDLEPSVEEARYNSCRPNMALGHPQLQQVTSPSRPSHAVIPADQDCSSRSLSSSAVESEATHQRKLRQKQLQQQFRERMEKQQVRVSTPSAEKSEAAPPAPPVTHSTPVTVSEPLLEKDFLAGVTQELIKTLEDNSEKWAVTPDAGDGVVKPSSRADPAQTSDTLALNNQMVTQNRTPHSVCHQKPQAKSGSWDLQTYSADQRTTGNWESHRKSQDMKKRKYDPS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Chicken
91
Zebrafish
90
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P43351 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
K41 Ubiquitination
Y104 Phosphorylation P00519 (ABL1) , A0A173G4P4 (Abl fusion) , A9UF07 (BCR/ABL fusion)
S129 Phosphorylation
K133 Acetylation
K135 Ubiquitination
S138 Phosphorylation
K141 Ubiquitination
Y171 Phosphorylation
S174 Phosphorylation
K177 Acetylation
K177 Ubiquitination
K190 Acetylation
K192 Acetylation
S199 Phosphorylation
Y205 Phosphorylation
S207 Phosphorylation
T223 Phosphorylation
S224 Phosphorylation
S251 Phosphorylation
K262 Acetylation
K274 Acetylation
T281 Phosphorylation
K286 Acetylation
S287 Phosphorylation
S299 Phosphorylation
T303 Phosphorylation
T318 Phosphorylation
K323 Acetylation
T335 Phosphorylation
K344 Acetylation
T397 Phosphorylation
K406 Acetylation
K411 Sumoylation
K412 Acetylation
K412 Sumoylation
K414 Acetylation
K414 Sumoylation

Research Backgrounds

Function:

Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.

PTMs:

Phosphorylated upon DNA damage by ABL1, and probably by ATM or ATR.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

The full-length protein forms heptameric rings. Interacts with ABL1. Interacts with RPA2; the interaction is direct and associates RAD52 with the RPA complex.

Family&Domains:

Belongs to the RAD52 family.

Research Fields

· Genetic Information Processing > Replication and repair > Homologous recombination.

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